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Geng, Chuan-Ying,Liu, Nian,Yang, Guang-Zhong,Liu, Ai-Jun,Leng, Yun,Wang, Hui-Juan,Li, Li-Hong,Wu, Yin,Li, Yan-Chen,Chen, Wen-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
At present, multiple myeloma (MM) remains an incurable disease and cologenic cells may be responsible for disease relapse. It has been proposed that CD20+/CD138- NCI-H929 cells could be hallmarks of MM clonogenic cells. Here, the immunology phenotype of NCI-H929 cells is described. Only a small population of CD20+/CD138- cells (<1%) was found in the NCI-H929 cell line, but CD20+/CD138- cells were not detected. We found that CD20+/CD138+ cells were able to exhibit cologenic capacity by colony formation assay and continuous passage culture. Proteins were analyzed by 1D-SDS-PAGE and TMT based quantitative differential liquid chromatography tandem mass spectrometry (LC-MS/MS). 1,082 non-redundant proteins were identified, 658 of which were differentially expressed with at least a 1.5-fold difference. 205 proteins in CD20+ cells were expressed at higher levels and 453 proteins were at lower levels compared with CD20- cells. Most proteins had catalytic and binding activity and mainly participated in metabolic processes, cell communication and molecular transport. These results proved that there are different biological features and protein expression profile between CD20+ and CD20- cells in the NCI-H929 cell line.
Shan, Zhao,Feng-Nian, Rong,Jie, Geng,Ting, Zhou Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Inhibitors of histone deacetylase activity are emerging as a potentially important new class of anticancer agents. In this study, we assessed the anticancer effects of valproic acid (VPA) on ovarian cancer in vitro and in vivo. Cultured SKOV3 cells were treated by VPA with different concentrations and time, then the effects on cell growth, cell cycle, apoptosis, and related events were investigated. A human ovarian cancer model transplanted subcutaneously in nude mice was established, and the efficacy of VPA used alone and in combination with diammine dichloroplatinum (DDP) to inhibit the growth of tumors was also assessed. Proliferation of SKOV3 cells was inhibited by VPA in a dose and time dependent fashion. The cell cycle distribution changed one treatment with VPA, with decrease in the number of S-phase cells and increase in G1-phase. VPA could significantly inhibit the growth of the epithelial ovarian cancer SKOV3 cells in vivo without toxic side effects. Treatment with VPA combined with DDP demonstrated enhanced anticancer effects. The result of flow cytometry (FCM) indicated that after VPA in vitro and in vivo, the expression of E-cadherin was increased whereas vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) were decreased. This study suggests that VPA could be a novel attractive agent for treatment of ovarian cancer.
A field determination method of D-T neutron source yields based on oxygen prompt gamma rays
Xiongjie Zhang,Bin Tang,Geng Nian,Haitao Wang,Lijiao Zhang,Yan Zhang,Rui Chen,Zhifeng Liu,Jinhui Qu Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.7
A field determination method for small D-T neutron source yield based on the oxygen prompt gamma rays was established. A neutron-gamma transport equation of the determination device was developed. Two yield field determination devices with a thickness of 20 mm and 50 mm were made. The count rates of the oxygen prompt gamma rays were calculated using three energy spectra processing approaches, which were the characteristic peak of 6.13 MeV, the overlapping peak of 6.92 MeV and 7.12 MeV, and the total energy area. The R-square of the calibration curve is better than 94% and the maximum error of the yield test is 5.21%, demonstrating that it is feasible to measure the yield of D-T neutron source by oxygen prompt gamma rays. Additionally, the results meet the requirements for field determination of the conventional D-T neutron source yield.