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IMPROVED PERTURBATION BOUND FOR ROBUST STABILITY IN LINEAR SYSTEM
Zhao, Zhang,Gan, Zhao Chan,Zhang, Hu Heng 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
The problem of robust stability of linear systems is investigated in time domain in this paper. For an asymptotically stable linear system, the maximum allowable perturbation bound (under which the system remains stable) is obtained by using Lyapunov method. A numerical example is given to show the perturbation bound obtained is better than ones in some literatures.
AN OPTIMAL ROBUST CONTROLLER DESIGN WITH OBSERVER
Gan, Zhao Chan,Yong, Yang 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
A new method for designing an optimal robust control system by using state plus state derivative feedback is presented in this paper. The system designed has low sensitivity for large parameter variations. For some states of system, a state observer is adopted to estimate the unmeasurable states, so that the implementation problem of control law with state plus state derivative is solved. An example is given, the simulation results show that the method suggested is effective.
Expression of the Proto-oncogene Pokemon in Colorectal Cancer - Inhibitory Effects of an siRNA
Zhao, Gan-Ting,Yang, Li-Juan,Li, Xi-Xia,Cui, Hui-Lin,Guo, Rui Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9
Objective: This study aimed to investigate expression of the proto-oncogene POK erythroid myeloid ontogenic factor (Pokemon) in colorectal cancer (CRC), and assess inhibitory effects of a small interference RNA (siRNA) expression vector in SW480 and SW620 cells. Methods: Semi-quantitative reverse transcription-polymerase chain reaction (PCR) and immunohistochemistry were performed to determine mRNA and protein expression levels of Pokemon in CRC tissues. Indirect immunofluorescence staining was applied to investigate the location of Pokemon in SW480 and SW620 cells. The siRNA expression vectors that were constructed to express a short hairpin RNA against Pokemon were transfected to the SW480 and SW620 cells with a liposome. Expression levels of Pokemon mRNA and protein were examined by real-time quantitative-fluorescent PCR and western blot analysis. The effects of Pokemon silencing on proliferation of SW480 and SW620 cells were evaluated with reference to growth curves with MTT assays. Results: The mRNA expression level of Pokemon in tumor tissues ($0.845{\pm}0.344$) was significantly higher than that in adjacent tumor specimens ($0.321{\pm}0.197$). The positive expression ratio of Pokemon protein in CRC (87.0%) was significantly higher than that in the adjacent tissues (19.6%). Strong fluorescence staining of Pokemon protein was observed in the cytoplasm of the SW480 and SW620 cells. The inhibition ratios of Pokemon mRNA and protein in the SW480 cells were 83.1% and 73.5% at 48 and 72 h, respectively, compared with those of the negative control cells with the siRNA. In the SW620 cells, the inhibition ratios of Pokemon mRNA and protein were 76.3% and 68.7% at 48 and 72 h, respectively. MTT showed that Pokemon gene silencing inhibited the proliferation of SW480 and SW620 cells. Conclusion: Overexpression of Pokemon in CRC may have a function in carcinogenesis and progression. siRNA expression vectors could effectively inhibit mRNA and protein expression of Pokemon in SW480 and SW620 cells, thereby reducing malignant cell proliferation.
Gan Junqing,Liu Shan,Zhang Yu,He Liangzi,Bai Lu,Liao Ran,Zhao Juan,Guo Madi,Jiang Wei,Li Jiade,Li Qi,Mu Guannan,Wu Yangjiazi,Wang Xinling,Zhang Xingli,Zhou Dan,Lv Huimin,Wang Zhengfeng,Zhang Yanqiao,Q 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
The functional role of microRNA-375 (miR-375) in the development of prostate cancer (PCa) remains controversial. Previously, we found that plasma exosomal miR-375 is significantly elevated in castration-resistant PCa (CRPC) patients compared with castration-sensitive PCa patients. Here, we aimed to determine how miR-375 modulates CRPC progression and thereafter to evaluate the therapeutic potential of human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes loaded with miR-375 antisense oligonucleotides (e-375i). We used miRNA in situ hybridization technique to evaluate miR-375 expression in PCa tissues, gain- and loss-of-function experiments to determine miR-375 function, and bioinformatic methods, dual-luciferase reporter assay, qPCR, IHC and western blotting to determine and validate the target as well as the effects of miR-375 at the molecular level. Then, e-375i complexes were assessed for their antagonizing effects against miR-375. We found that the expression of miR-375 was elevated in PCa tissues and cancer exosomes, correlating with the Gleason score. Forced expression of miR-375 enhanced the expression of EMT markers and AR but suppressed apoptosis markers, leading to enhanced proliferation, migration, invasion, and enzalutamide resistance and decreased apoptosis of PCa cells. These effects could be reversed by miR-375 silencing. Mechanistically, miR-375 directly interfered with the expression of phosphatase nonreceptor type 4 (PTPN4), which in turn stabilized phosphorylated STAT3. Application of e-375i could inhibit miR-375, upregulate PTPN4 and downregulate p-STAT3, eventually repressing the growth of PCa. Collectively, we identified a novel miR-375 target, PTPN4, that functions upstream of STAT3, and targeting miR-375 may be an alternative therapeutic for PCa, especially for CRPC with high AR levels.
Gan Hui,Zhang Li,Chen Hui,Xiao Han,Wang Lu,Zhai Xuan,Jiang Ning,Liang Ping,Zheng Shuyue,Zhao Jing 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
The NLRC4 inflammasome, a member of the nucleotide-binding and oligomerization domain-like receptor (NLR) family, amplifies inflammation by facilitating the processing of caspase-1, interleukin (IL)–1β, and IL-18. We explored whether NLRC4 knockdown alleviated inflammatory injury following intracerebral hemorrhage (ICH). Furthermore, we investigated whether NLRC4 inflammasome activation can be adjusted by the regulator of G protein signaling 2/leucine-rich repeat kinase-2 pathway. Fifty microliters of arterial blood was drawn and injected into the basal ganglion to simulate the ICH model. NLRC4 small interfering RNAs (siRNAs) were utilized to knockdown NLRC4. An LRRK2 inhibitor (GNE7915) was injected into the abdominal cavity. Short hairpin (sh) RNA lentiviruses and lentiviruses containing RGS2 were designed and applied to knockdown and promote RGS2 expression. Neurological functions, brain edema, Western blot, enzyme-linked immunosorbent, hematoxylin and eosin staining, Nissl staining, immunoprecipitation, immunofluorescence assay and Evans blue dye extravasation and autofluorescence assay were evaluated. It was shown that the NLRC4 inflammasome was activated following ICH injury. NLRC4 knockdown extenuated neuronal death, damage to the blood-brain barrier, brain edema and neurological deficiency 3 days after ICH. NLRC4 knockdown reduced myeloperoxidase (MPO) cells as well as tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β and IL-18 following ICH. GNE7915 reduced pNLRC4 and NLRC4 inflammasome activation. RGS2 suppressed the interaction of LRRK2 and NLRC4 and NLRC4 inflammasome activation by regulating pLRRK2. Our study demonstrated that the NLRC4 inflammasome may aggravate the inflammatory injury induced by ICH and that RGS2/LRRK2 may relieve inflammatory injury by restraining NLRC4 inflammasome activation.
Symplectic analysis of functionally graded beams subjected to arbitrary lateral loads
Zhao, Li,Gan, Wei Z. Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.1
The rational analytical solutions are presented for functionally graded beams subjected to arbitrary tractions on the upper and lower surfaces. The Young's modulus is assumed to vary exponentially along the thickness direction while the Poisson's ratio keeps unaltered. Within the framework of symplectic elasticity, zero eigensolutions along with general eigensolutions are investigated to derive the homogeneous solutions of functionally graded beams with no body force and traction-free lateral surfaces. Zero eigensolutions are proved to compose the basic solutions of the Saint-Venant problem, while general eigensolutions which vary exponentially with the axial coordinate have a significant influence on the local behavior. The complete elasticity solutions presented here include homogeneous solutions and particular solutions which satisfy the loading conditions on the lateral surfaces. Numerical examples are considered and compared with established results, illustrating the effects of material inhomogeneity on the localized stress distributions.
Symplectic analysis of functionally graded beams subjected to arbitrary lateral loads
Li Zhao,Wei Z. Gan 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.1
The rational analytical solutions are presented for functionally graded beams subjected to arbitrary tractions on the upper and lower surfaces. The Young’s modulus is assumed to vary exponentially along the thickness direction while the Poisson’s ratio keeps unaltered. Within the framework of symplectic elasticity, zero eigensolutions along with general eigensolutions are investigated to derive the homogeneous solutions of functionally graded beams with no body force and traction-free lateral surfaces. Zero eigensolutions are proved to compose the basic solutions of the Saint-Venant problem, while general eigensolutions which vary exponentially with the axial coordinate have a significant influence on the local behavior. The complete elasticity solutions presented here include homogeneous solutions and particular solutions which satisfy the loading conditions on the lateral surfaces. Numerical examples are considered and compared with established results, illustrating the effects of material inhomogeneity on the localized stress distributions.