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Khan, Fazlurrahman,Manivasagan, Panchanathan,Pham, Dung Thuy Nguyen,Oh, Junghwan,Kim, Shin-Kwon,Kim, Young-Mog Elsevier 2019 Microbial pathogenesis Vol.128 No.-
<P><B>Abstract</B></P> <P> <I>Pseudomonas aeruginosa</I> is an opportunistic human pathogen which exhibits its property of pathogenesis due to several factors, including the formation of biofilm and production of several virulence factors. Development of resistance properties against antibiotics leads to the discovery of certain alternative strategies to combat its pathogenesis. In the present study, a highly stable, biocompatible and water soluble nanocomposites (NCs) are synthesized from chitosan (CS) and the polypyrrole (PPy). The resultant chitosan-polypyrrole nanocomposites (CS-PPy NCs) inhibit the establishment of biofilm and also eradicate the preformed matured biofilm formed by <I>P. aeruginosa</I>. CS-PPy NCs inhibit the hemolytic and protease activities of <I>P. aeruginosa</I>. The NCs significantly reduce the production of many virulence factors such as pyocyanin, pyroverdine and rhamnolipid. CS-PPy NCs also suppress the bacterial motility such as swimming and swarming. The present study showed that highly stable CS-PPy NCs act as a potent antibiofilm and antivirulence drug for the treatment of <I>P. aeruginosa</I> infection.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CS-PPy is a water soluble and highly stable nanocomposites. </LI> <LI> CS-PPy NCs inhibits the formation of biofilm of <I>P. aeruginosa.</I> </LI> <LI> CS-PPy NCs exhibits antihemolytic and antivirulence properties to <I>P. aeruginosa.</I> </LI> <LI> CS-PPy NCs impairs the motilities properties of <I>P. aeruginosa.</I> </LI> <LI> CS-PPy NCs can be used as a potent antibiofilm and antivirulence drug. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Khan, Fazlurrahman,Lee, Jang-Won,Manivasagan, Panchanathan,Pham, Dung Thuy Nguyen,Oh, Junghwan,Kim, Young-Mog Academic Press 2019 Microbial pathogenesis Vol.135 No.-
<P><B>Abstract</B></P> <P>The infection caused by <I>Pseudomonas aeruginosa</I> is a serious concern in human health. The bacterium is an opportunistic pathogen which has been reported to cause nosocomial and chronic infections through biofilm formation and synthesis of several toxins and virulence factors. Furthermore, the formation of biofilm by <I>P. aeruginosa</I> is known as one of the resistance mechanisms against conventional antibiotics. Natural compounds from marine resources have become one of the simple, cost-effective, biocompatible and non-toxicity for treating <I>P. aeruginosa</I> biofilm-related infections. Furthermore, hybrid formulation with nanomaterials such as nanoparticles becomes an effective alternative strategy to minimize the drug toxicity problem and cytotoxicity properties. For this reason, the present study has employed chitosan oligosaccharide for the synthesis of chitosan oligosaccharide-capped gold nanoparticles (COS-AuNPs). The synthesized COS-AuNPs were then characterized by using UV–Visible spectroscopy, Dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), Field emission transmission electron microscopy (FE-TEM), and Energy dispersive X-ray diffraction (EDX). The synthesized COS-AuNPs were applied for inhibiting <I>P. aeruginosa</I> biofilm formation. Results have shown that COS-AuNPs exhibited inhibition to biofilm as well as eradication to pre-existing mature biofilm. Simultaneously, COS-AuNPs were also able to reduce bacterial hemolysis and different virulence factors produced by <I>P. aeruginosa</I>. Overall, the present study concluded that the hybrid nanoformulation such as COS-AuNPs could act as a potential agent to exhibit inhibitory properties against the <I>P. aeruginosa</I> pathogenesis arisen from biofilm formation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> COS-AuNP was synthesized and subjected to characterization by FTIR, EDX, XRD and FE-TEM. </LI> <LI> COS-AuNP inhibited <I>P. aeruginosa</I> biofilm formation and eradicated the mature biofilm. </LI> <LI> COS-AuNP inhibited hemolytic activity, virulence factors production and motilities. </LI> </UL> </P>