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Abdel Naser Zaid,Murad N. Abualhasan,Nidal Jaradat,Mohammed Marar,Kenza Mansoor,Fadi Qa’dan 한국약제학회 2016 Journal of Pharmaceutical Investigation Vol.46 No.6
Carvone volatile oil is a commercial product that is widely used in the food, cosmetic and pharmaceutical industry due to its fragrance, flavoring and medicinal properties. A validated HPLC method for the qualitative and quantitative analysis of this oil in raw and commercial products is not available in the literature. The aim of this study is to develop a validated new analytical HPLC method for the estimation of Carvone in raw materials and suppositories. The HPLC analysis was carried out using a reversed phase-C18 (250 9 4.0 mm, 5 lm) column. Chromatographic analysis was carried out using a Shimadzu HPLC system equipped with an LC-2010 pump, LC-2010 autosampler, LC-2010 oven, and LC-2010 UV– VIS detector. The mobile consists of two phases Methanol and 1.0 mL of trifluoroacetic acid in 1000 mL filtered and degas HPLC water. The developed HPLC analytical method was simple and was validated in accordance with FDA and ICH guidelines and showed good linearity, accuracy, precision, selectivity and the system suitability results were within the acceptance criteria. This new analytical method could be used to screen the quality of the oil in formulation studies of the final product formulations and can be applied for routine analysis of carvone in raw material as well as in pharmaceutical suppositories and can be applied in any lab and industrial batch quality control.
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