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뉴질랜드의 종자품질관리체계와 향후 우리나라의 품질관리 개선 방향
김진기,유남희,나의식 한국국제농업개발학회 2001 韓國國際農業開發學會誌 Vol.13 No.4
뉴질랜드의 종자산업은 종자개량, 품종보호, 종자생산, 종자검사, 종자보증 및 종자교역 등 여러분야의 연구와 실무를 통하여 종자품질관리에 선구적 입장을 유지하여 왔으며 신품종의 개발과 합리적인 증식 및 관리체계를 통하여 양질의 종자공급능력을 발전시켜 왔다. 1970 년대 이전까지는 품종개량 및 종자품질관리는 정부주도로 이루어졌으나 현재는 정부가 직접적인 관여에서 점차 간접적인 위임형태로 바꾸어 종자품질관리 관련 업무 일부만 남기고 모두 민영화가 추진되었다. 위임받은 업무에 대한 책임은 위임받은 조직이 지며 정부는 품질관리 체계를 정기적으로 감독하고 감시하는 자세로 전환하였다. 이와 같은 변화에 따라 종자품질관리체계의 하부조직은 대부분 독립적인 예산체계를 구축하였고 업무도 좀 더 독립성을 갖게 되는 효과를 보았다. 그러나 계속적인 정부 지원의 절감과 새로운 조직의 지나친 상업적 운영은 전문인력이 점차적인 감소와 연구개발활동의 축소라는 부작용도 있다. 우리나라의 종자산업발전을 위하여 공공기관이나 민간종묘회사 어느 쪽이든 품종개량과 종자품질관리활동이 활발하게 이루어져야 할 것임은 말할 것도 없다. 그러나 앞으로 국내 종자시장의 변화나 재정적인 이유로 품종개량과 종자품질관리체계에 어떤 개선이 요구된다면 뉴질랜드가 재정적인 동기에서 도출하였던 공공기관의 기능과 역할의 분담조정, 인력의 조직화와 민간종묘회사의 참여방식 및 종자의 상품화 체계구축 등 선험적 체계가 다소라도 참고가 되지 않을까 생각된다. New Zealand has developed a very efficient seed quality control scheme. In New Zealand, cultivars are now developed under contract to the CDMU(Cultivar Development and Maintenance Unit) of AgResearch(NZ Pastoral Agriculture Research Institute Limited). MAF (Ministry of Agriculture and Forestry) is empowered to provide seed certification services, and the scheme is administered by MAF Quality Management using procedures that are in accordance with OECD standards. New Zealand has no seed laws and participation in the seed production industry is entirely voluntary. although participants must adhere strictly to the rules and procedures involved. National Seed Laboratory (NSL) has played a central role in prividing seed quality assessment. Recently all sectors of the seed industry in New Zealand have been reformed and become more commercialized with users-pay policy. The services have been affected by moves to reduce government involvement in its affair and to eliminate government subsidies. However, this has led to reduction of research activities and personnel in seed technology. The future directions of seed quality control system in Korea could refer to New Zealand scheme.
Direct Multipropagation through Organogenesis from Nodal Explants of Anoectochilus formosanus Hayata
Eui Shik Rha,Hyun Soon Kim,Nam Hee Yoo 한국자원식물학회 1998 Plant Resources Vol.1 No.1
To establish direct multipropagation through organogenesis from nodal explants of Anveilochilus formosunus Hayata, the nodes were cultured on LS medium containing various concentrations of 6-benzyladenine(BA). High plant regeneration and adventitious bud formation were obtained from supplemented with 4.0mg/l of BA. Plant height was promoted by adding 0.3% activated charcoal. Plantlet regeneration capacity from nodes was depended on nodal parts on the stem, upper position was the best comparing with intermediate and lower.
Genotype Difference of Plant Regeneration from Dormant Bud Culture in Colocasia esculenta Schott.
Eui Shik Rha,Nam Hee Yoo,Hyun Soon Kim 한국자원식물학회 1999 Plant Resources Vol.2 No.2
This study was conducted to obtain the basic breeding information of Colocasia esculenta Schott. Effect of supplemental plant growth regulators and genotype difference were investigated on dormant bud tissue for proliferation. The plant regeneration ratio, plant height and root length were the best upon mixed treatment of 0.8mg/L IAA and 2.0mg/L zeatin. Both leaf weight and root weight were heavy upon culture in a dark condition. The leaf and root weights were heaviest in 6% sucrose concentrations. In several collected area the heaviest one was Binnangxin and then in the order of Suwon, Wanju and Puan. Genotype differences of tuber diameter and tuber weight were found in Suwon. Tuber weight was found in the order of Suwon(862mg)>Wanju(723mg)>Puan(649mg)>Binnangxin (424mg).
In vitro Tissue Culture of Aloe arborescens Mill
Eui Shik Rha,Hyun Soon Kim,Seung Yeob Lee 한국자원식물학회 1998 Plant Resources Vol.1 No.2
Aloe in vitro culture was attempted to induce callus and regeneration ability from different explant sources onto MS medium with 0.5mg/l NAA plus 1.0mg/l BA. Anthers that no developed any callus and plant regeneration, while only four out of 274 filament explants induced calli at cut edge without regenerated plants. Twenty ovary explants regenerated four direct plantlets without via callus from the base of epidermal tissues. Regenerated plants on the root tip gave 2n=14 of chromosome numbers.
Characteristics of Phytolith on Rice Leaf
Rha, Eui-Shik,Kim, Jin-Key The Korean Society of Crop Science 1998 Korean journal of crop science Vol.43 No.4
Silica bodies (phytoliths) are becoming of wide use for pedology, archaeology, paleobotany and paleoecology in botany. This study investigated morphological differences of silica bodies in the lamina of wild, indica type, and japonica type rice. Phytoliths in the epidermis of lamina showed noticeable difference among tested plants. Besides, there were also significant differences in the shape and distribution of the silica bodies around stomata and trichomes. Silica bodies in the lamina of the rice plants could be used to classify subspecies of Oryza genus.
Characteristics of Phytolith on Rice Leaf
Eui Shik Rha,Jin Key Kim 韓國作物學會 1998 Korean journal of crop science Vol.43 No.4
Silica bodies (phytoliths) are becoming of wide use for pedology, archaeology, paleobotany and paleoecology in botany. This study investigated morphological differences of silica bodies in the lamina of wild, indica type, and japonica type rice. Phytoliths in the epidermis of lamina showed noticeable difference among tested plants. Besides, there were also significant differences in the shape and distribution of the silica bodies around stomata and trichomes. Silica bodies in the lamina of the rice plants could be used to classify subspecies of Oryza genus.
RT - PCR 을 이용한 BaYMV 와 BaMMV 의 외피단백질 동정과 유전자 염기서열분석
나의식 한국유전학회 2001 Genes & Genomics Vol.23 No.2
Barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) were detected by double antibody sandwich-ELISA in barley (Hordeum vulagre L.) plants with virus-like symptoms collected from eight locations at Buan, Korea. Barley plants were either single- or mix-infected with BaYMV and BaMMV. BaYMV showed higher infection (90.79%) than BaMMV (54.12%). The reverse transcription polymerase chain reaction (RT-PCR) was used for detection and identification of BaYMV and BaMMV. The coat proteins of the two virus was distinguished by two pairs of primes that are s13 and s14, s15 and s17. PCR product for BaYMV was produced from sequences between 1078 and 872 by and that for BaMMV from sequences below 872 bp. The sequencing of three individual DNA clones in pT7Blue vector revealed that BaYMV coat protein contains 297 and BaMMV contains 251 amino acids. Homology of nucleotide and amino acid sequence between coat proteins of BaYMV and BaMMV was 45.6 and 34.9%, respectively. These results suggest that barely viruses BaYMV and BaMMV could be diagnosed rapidly and accurately by using RT-PCR modified primers (BaYMV: s14+s13, BaMMV: s17+s15).