신 콩품종 및 계통의 콩모자이크바이러스에 대한 저항성 검정

조의규 安東大學校 農業科學技術硏究所 1995 農業科學技術 硏究論文集 Vol.2 No.-

수원 작물시험장과 영남작물시험장에서 1995년 검정 의뢰한 모든 콩품종이나 계통은 콩모자이크병에 이병성인 품종으로 판명되었다. All soybean accessions provided by Crop Experiment Station and Youngnam Agricultural Experiment station in 1995 were susceptible to an isolate of soybean mosaic virus, producing mosaic symptoms. No resistant soybean accessions was found to mosaic either to necrotic disease of soybean.

지역의무 공동도급 수주에 의한 공사현장 운영실태 연구

임의수,최응규,조용훈 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

Since the systematic researches on the operation for compulsory local joint contract are not enough, it is required to make efforts the sound activation of this system. This study analyzes and examines the problems occurred at the operation of construction field due to compulsory local joint contract. And tries to suggest effective operational guidelines

Dimethomorph처리에 의한 고추역병 방제효과

이영근,조의규 安東大學校 農業科學技術硏究所 1995 農業科學技術 硏究論文集 Vol.2 No.-

Dimethomorph의 고추역병에 대한 방제효과를 두가지 약제처리 방법에 의하여 metalaxylcopper oxychloride, propamocarb hydrochloride, ICIA5504의 방제효과와 비교하였다. 1. 약제 현탁액을 고추의 잎과 줄기에 살포하였을 때, 4개 약제 모두 고추에 대한 약해는 인정되지 않았다. Metalaxyl-Cu 현탁액을 고추의 근권토양에 관주하였을 때, 고추의 잎에 괴저형 점무늬가 발생하였다. 나머지 3개 약제의 현탁액을 토양관주한 고추에서는 어떤 약해증상도 나타나지 않았다. 2. Oatmeal 배지 상에서 역병균사에 대한 발육억제효과는 dimethomorph와 metalaxyl-Cu가 우수하였다. 3. 4종의 약제 모두 경엽살포에 의한 고추역병방제효과는 크지 않았다. 4. Dimethomorph 500-2,000배액을 고추의 근권토양에 관주하고 줄기에 역병균사를 접종하였을 때, 약제처리 7주 후까지 역병의 병반진적이 억제되었다. Control effect of dimethomorph on red-pepper fruit rot(Phytophthora capsici) were compared with the effects of metalaxyl-copper oxychloride, propamocarb hydrochloride and ICIA5504 by spray and soil-drenching method. Any damage was not observed on the red-pepper plants sprayed with four kind of fungicides. By soil-drenching method, small necrotic spots were observed on leaves of the plants soil-dranched with metalaxyl-copper but any damage of the plants treated with other fungicides was not observed. On oatmeal agar media, inhibition effect of dimethomorph and metalaxyl-copper on growth of the pathogenic fungi were excellent. However, the control effects of the chemicals on red-pepper fruit rot were not significant by spray application. When dimethomorph suspensions(500 to 2,000 times) were poured onto the rhizosphere soil, developments of black rotten symptoms were inhibited untill seven weeks after the chemical treatment.

출혈성 설사증을 보인 astrovirus 감염증의 신생아실 집단발생

이종윤,이재규,정은희,조동희,김의종 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

Effects of Light on Production of Toxins by Helminthosporium victoriae and H. carbonum

Cho Eui Kyoo,Scheffer Robert P. Korean Society of Applied Entomology 1975 한국식물보호학회지 Vol.14 No.2

Helminthosporium vicotoriae and H. carbonum were grown under fluorescent plus incandescent lights, or in darkness, with several different temperature regimes. There was little or no effect of light on toxin production by H. victoriae. Light-grown cultures of H. carbonum had significantly higher titres of host-specific toxin than did dark-grown cultures. Light had no significant effect on growth of either fungus. Maximum titres of host-specific toxins from both fungi were evident at the time maximum growth was reached. Minimum pH values in Fries modified medium occurred at the time of, or slightly before maximum level of toxin was reached.

Cowpea mosaic virus from Vegetable Soybeans in Korea

Cho, Eui-Kyoo,Lee, Sin-Ho The Korean Society of Plant Pathology 2003 Plant Pathology Journal Vol.19 No.3

Ninety samples showing mosaic symptoms on soybean (Glycine max) cv. Sukryangputkong were collected from the Cheongsongkun area, Kyungbuk province in Korea. Initially, DAS-ELISA was conducted far detection of Soybean mosaic virus (SMV). Negative samples were chosen at random and mechanically inoculated on soybean cv. Buffalo, which reported not to produce mosaic symptoms when mechanically inoculated with SMV. An isolate of SMV, designated as B-1, from Buffalo showing mosaic and mottle symptoms was used for identification and biological characterization of the causal vim. The purified B-1 isolate had spherical particles of approximately 24nm. It positively reacted with the antiserum against Cowpea mosaic virus (CPMV) but not with Cucumber mosaic virus (CMV) and SMV antisera. CPMV was newly isolated from soybean and had been characterized by host range and by serological and electron microscopic methods. Results of this study suggest that CPMV is the possible cause of mosaic disease in vegetable soybean and that based on sympto-matology, a difference between the typical mosaic and rugose symptoms caused by SMV and CPMV was observed. This is first report of CPMV from soybean in Korea.

한국 大豆 바이러스의 分類, 同定에 關한 硏究 1 : 一種의 大豆 바이러스에 관한 硏究

Cho Eui Kyoo,Bong Jo Chung 한국응용곤충학회 1976 한국응용곤충학회지 Vol.15 No.2

광교, 동북태, 강림, 육우3호, 은대두등과 같은 대리장려품종이 바이러스에 의하여 심하게 이병되었다. 이 병은 주로 모자익병의 발생이 많은 강원, 경기지방에서 발병이 심하였으나 모자익병이 심하지 않은 전남 등 남부지방에서도 발병되고 있다. 병징으로 보아 tobacco ringspot virus에 의한 대두의 피해와 유사한 것으로 보였으나 지표식물검정과 혈청검정에 의하여 조사한 결과 모두 부정적이었으며 대두품종에 따른 이병정도의 상이, 품종과 접종원에 의한 병징의 변이가 많았다. 이병주에서 분리되는 병징형은 Mottling과 necrosis였으며 지금까지의 연구결과 이 대두병해는 모자익바이러스(SMV)의 계통 내지는 tobacco ringspot virus 이외의 두류바이러스의 복각감염에 의한 것으로 생각할 수 있으나 SMV의 계통에 의한 피해일 가능성이 더욱 유력시되고 있다. Leading soybean cultivars such as Kwanggyo, Yugu No.3, Dongbugtae, Gangrim, and Eundaedu were heavily diseased by a virus in Korea. The disease was most severe in the northern provinces where soybean mosaic virus also occurrs, but the disease has also been observed in other provinces where soybean diseases are less prevalent. The disease symptoms were similar to bud blight caused by tobacco ringspot virus, but this was not confirmed in inoculation tests on indicator plants and serological experiments. There were some differences in varietal susceptibility to the disease, with symptom variation depending on the soybean cultivar and source of inoculm. Disease symptoms on infected soybean plants were mottling and necrosis. The present results, therefore, indicate some strains of SMV or a mixture of legume viruses may or may not be responsible for the disease.

Plant Virus Replication, Assembly And Multiplication In Relation To Cellular Sites of Host Plant

Cho, Eui-Kyoo 安東大學 1993 安東大學 論文集 Vol.15 No.1

대부분의 식물 바이러스는 단일나선상으로 된(Single-stranded)RNA핵산으로 구성된 것으로 알려져 왔다. 그러나, 1980년대 처음으로 발견된 제미나이바이러스(Geminivirus)의 게놈(genome)이 단일나선상으로 구성된 DNA로 이루어진 것으로 밝혀짐에 따라 1960년대에 이미 발견된 2중나선상(double-stranded)DNA게놈을 가진 꽃양배추바이러스(Gawlifower Mosaic Virus)와 이중나선구조를 가진(double stranded)RNA게놈의 벼오갈병바이러스(Rice dwarf virus)와 더불어 외부게놈이 어떻게 진핵세포(Eukaryote)의 게놈에 영향을 미치며 표현되는가를 연구하기 위한 재료로서의 중요성이 부각되고 있다. 특히 최근에 와서 유전공학기법을 이용한 작물의 개량을 위한 목적으로 비교적 용이한 연구재료로서 그 중요성이 부각되고 있으므로 식물 바이러스의 특성과 더불어 기주식물 세포기관과 바이러스게놈의 복제와 재구성과 관련된 자료를 정리하여 보았다. 지금까지 보고된 결과를 요약하면 1) 바이러스가 포함하고 있는 게놈은 바이러스입자의 형태에 따라 변이가 있는 바 TMV와 같이 간상인 바이러스는 5%, 순무바이러스와 같은 다면체바이러스 즉, 구형바이러스는 게놈의 량이 15-45%이며 상치괴저바이러스와 같은 간균형바이러스는 바이러스입자의 20%가 RNA게놈이다. 이러한 바이러스의 기주범위에 관한 조사는 완전한 식물체에 바이러스를 인공접종하여 바이러스의 식물세포 침입과 동시에 일어나는 탈각(Uncoating)현상과 바이러스복제과정이 추적되었으나, 식물체의 원형질체(Protoplast)의 나출과 조직배양기술이 발전함에 따라 원형질체에 직접 바이러스를 접종하여 바이러스게놈의 기주식물세포에 대한 발현기각이 연구하게 되어 바이러스에 대한 기주범위도 그 기주를 새로 정립할 필요성이 있을것으로 보이며 세포의 기관중에서 엽록체(Chloroplast)와 plasma membrane이 식물바이러스의 복제와 구성에 큰 관련이 있을것으로 보이지만 아직 이 방면에 관한 연구가 미지의 상태임으로 앞으로의 연구결과가 기대되고 있다.

콩 壞疸病을 일으키는 콩모자이크바이러스 系統分布

Eui Kyoo Cho(趙義奎),Kil Woog Chung(鄭吉雄) 한국육종학회 1986 한국육종학회지 Vol.18 No.2

Five strains of soybean mosaic virus SMV-G1, SMV-G2, SMV-G5, SMV-G6 and SMV-G7 were identified from 220 soybean leaf samples collected in Kyunggi, Chungnam, Kyungnam and Cheonnam provinces in Korea. The strains G5, G6, and G7 occupied 66% of all isolates tested, which can cause necrotic disease in some soybean cultivars such as Kwanggyo, Marshall, and Buffalo. Seed transmission was observed only from the seed samples grown in Kangweon province where the soybean mosaic virus disease occurred severely but no seed transmission from the seed samples grown in Cheonam and Kyungnam. The seed samples were Suweon 120 which each upland crop division of the regional Rural Development Administration provided with.

Effects of Light on Production of Toxins by Helminthosporium victoriae and H. carbonum

Eui Kyoo Cho,Robert P. Scheeffer 한국응용곤충학회 1975 한국응용곤충학회지 Vol.14 No.2

Helminthosporium vicotoriae and H. carbonum were grown under fluorescent plus incandescent lights, or in darkness, with several different temperature regimes. There was little or no effect of light on toxin production by H. victoriae. Light-grown cultures of H. carbonum had significantly higher titres of host-specific toxin than did dark-grown cultures. Light had no significant effect on growth of either fungus. Maximum titres of host-specific toxins from both fungi were evident at the time maximum growth was reached. Minimum pH values in Fries modified medium occurred at the time of, or slightly before maximum level of toxin was reached.