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- 저자 : Eric Wers (검색결과 2 건)
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New hybrid agarose/Cu-Bioglass® biomaterials for antibacterial coatings
Eric Wers,Bertrand Lefeuvre 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.8
Agarose hydrogels, combined with 45S5 Bioglass®, were elaborated to serve as copper delivery systems. Our aim was to study the antibacterial properties of these hydrogels. The results show that the amount of glass does not influence the stiffness properties, but it improves the hydrophilicity and the swelling profile of agarose hydrogel. Two bacterial strains, Bacillus sp. 4J6 and Pseudomonas aeruginosa sp. PAO1, were chosen. Their retention on the substrates was analyzed by confocal laser scanning microscopy. The mechanical characteristics and the release of copper have an effect on the bacterial adhesion and the biofilm formation. All the obtained results indicate that these hydrogels could be adapted to a potential application to the antibacterial coatings.
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Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations
Hassane Oudadesse,Eric Wers,Xuang Vuong Bui,Claire Roiland,Imane Akhiyat,Amany Mostafa,Hassan Chaair,Hicham Benhayoune,Joel Fauré,Pascal Pellen-Mussi,Bruno Bureau 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.9
Bioactive glass 46S6 and biodegradable therapeutic polymer (Chitosan: CH) have been elaborated to form 46S6-CH composite by freeze-drying process. The kinetics of chemical reactivity and bioactivity at the surface were investigated by using physicochemical techniques, particularly solid-state MAS-NMR. Immortalized cell line used to construct multicellular spheroids was employed as three-dimensional (3D) cell cultures for in vitro studies. Obtained results showed a novel structure of the composite; the chemical treatment (ultrasound, magnetic stirring, freeze drying process and lyophilization) led the bioactive glass particles to be loaded in the chitosan-based materials. 29Si and 31P MAS-NMR results showed the emergence of two new species, QSi 3(OH) and QSi 4, which are characteristic of the vitreous network dissolution in simulated body fluid (SBF). MAS-NMR also confirmed the formation of amorphous calcium phosphate (ACP) at the surface of the initial 46S6-CH. Three-dimensional (3D) cell cultures highlighted the effect of chitosan, where the cell viability reached up to 78% in 46S6-CH composite and up to 67% in 46S6. The association of (CH) and bioactive glass (BG) matrix promotes a highly significant bioactivity, demonstrating surface bone formation and satisfactory behavior in biological environment.
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