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Jesu´ s Ramo´n-Sierra,Jose´ Luis Martı´nez-Guevara,Luis Pool-Yam,Denis Magan˜a-Ortiz,Alejandro Yam-Puc,Elizabeth Ortiz-Va´zquez 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.7
Antimicrobial effects of Melipona beecheiihoney have been attributed to diverse factors, in this sense,certain components such as proteins and phenolics couldexplain relevant aspects of its antimicrobial activity. Theaim of this study was to evaluate the antibacterial activityof phenolic and protein extracts from M. beecheii honeyagainst two bacterial pathogens: Staphylococcus aureusand Escherichia coli. With respect to phenolic content,HPLC analysis allowed the identification of phenolic acidslike chlorogenic acid, caffeic acid, and flavonoids likecatechin, myricetin, quercetin and apigenin. On the otherhand, seven bands with molecular weight from 7.6 to95 kDa were detected in protein extract by SDS-PAGEsystem. It was determined the antibacterial activity of bothextracts, with MICs lower than 145 lg/mL and 60 lg/mLfor the phenolic and protein extracts respectively. Theseresults indicate that phenolic and protein components of M. beecheii honey contribute significantly to the antibacterialactivity.