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Nitrosomonas sp. Based Biosensor for Ammonium Nitrogen Measurement in Wastewater
Merlin Raud,Erki Lember,Eerik Jõgi,Timo Kikas 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.5
A bacterial culture of Nitrosomonas sp. was isolated from a nitrifying biofilm to construct a biosensor for ammonium nitrogen ( ) measurements in high ammonia wastewaters. The pure culture of microorganisms was immobilized into agarose gel matrix to attain a stable biosensor with a long service life. Biosensors were calibrated using (NH4)2SO4 solution and a steady-state method. Subsequently, several experiments with synthetic and industrial wastewaters were conducted. A linear range up to 20 mg/L of , and sensitivities between 0.030and 0.036 were gained with biosensors. During 14 days of stable service life of the Nitrosomonas sp. biosensors,variation of the signal was less than 7%. Response times of biosensors were 15 ~ 25 min, while recovery times were up to 25 min. Measurements with high ammonia content synthetic and industrial wastewaters were conducted, and 8.3 and 5.6% over estimation of was gained,respectively, compared with results of Nessler method. In spite of the small overestimation, the biosensor based on a pure culture of Nitrosomonas sp. and calibrated with (NH4)2SO4 is suitable for the analysis of in high ammonia content wastewaters.
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