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- 검색키워드
- 저자 : Duve, Khrystyna (검색결과 3 건)
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Widiyanto Sri Nanan,Sulaiman Syahril,Duve Simon,Marwani Erly,Nugrahapraja Husna,Diningrat Diky Setya 한국식물생명공학회 2023 JOURNAL OF PLANT BIOTECHNOLOGY Vol.50 No.2
Water scarcity decreases the rate of photo- synthesis and, consequently, the yield of banana plants (Musa spp). In this study, transcriptome analysis was performed to identify photosynthesis-related genes in banana plants and determine their expression profiles under water stress conditions. Banana plantlets were in vitro cultured on Murashige and Skoog agar medium with and without 10% polyethylene glycol and marked as BP10 and BK. Chlorophyll contents in the plant shoots were determined spectrophotometrically. Two cDNA libraries generated from BK and BP10 plantlets, respectively, were used as the reference for transcriptome data. Gene ontology (GO) enrichment analysis was performed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) and visualized using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway prediction. Morphological obser- vations indicated that water deficiency caused chlorosis and reduced the shoot chlorophyll content of banana plantlets. GO enrichment identified 52 photosynthesis-related genes that were affected by water stress. KEGG visualization revealed the pathways related to the 52 photosynthesis- related genes and their allocations in four GO terms. Four, 12, 15, and 21 genes were related to chlorophyll biosynthesis, the Calvin cycle, the photosynthetic electron transfer chain, and the light-harvesting complex, respectively. Differentially expressed gene (DEG) analysis using DESeq revealed that 45 genes were down-regulated, whereas seven genes were up-regulated. Four of the down-regulated genes were responsible for chlorophyll biosynthesis and appeared to cause the decrease in the banana leaf chlorophyll content. Among the annotated DEGs, MaPNDO, MaPSAL, and MaFEDA were selected and validated using quantitative real-time PCR.
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Sri Nanan Widiyanto,Syahril Sulaiman,Simon Duve,Erly Marwani,Husna Nugrahapraja,Diky Setya Diningrat The Korean Society of Plant Biotechnology 2023 JOURNAL OF PLANT BIOTECHNOLOGY Vol.50 No.2
Water scarcity decreases the rate of photosynthesis and, consequently, the yield of banana plants (Musa spp). In this study, transcriptome analysis was performed to identify photosynthesis-related genes in banana plants and determine their expression profiles under water stress conditions. Banana plantlets were in vitro cultured on Murashige and Skoog agar medium with and without 10% polyethylene glycol and marked as BP10 and BK. Chlorophyll contents in the plant shoots were determined spectrophotometrically. Two cDNA libraries generated from BK and BP10 plantlets, respectively, were used as the reference for transcriptome data. Gene ontology (GO) enrichment analysis was performed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) and visualized using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway prediction. Morphological observations indicated that water deficiency caused chlorosis and reduced the shoot chlorophyll content of banana plantlets. GO enrichment identified 52 photosynthesis-related genes that were affected by water stress. KEGG visualization revealed the pathways related to the 52 photosynthesisr-elated genes and their allocations in four GO terms. Four, 12, 15, and 21 genes were related to chlorophyll biosynthesis, the Calvin cycle, the photosynthetic electron transfer chain, and the light-harvesting complex, respectively. Differentially expressed gene (DEG) analysis using DESeq revealed that 45 genes were down-regulated, whereas seven genes were up-regulated. Four of the down-regulated genes were responsible for chlorophyll biosynthesis and appeared to cause the decrease in the banana leaf chlorophyll content. Among the annotated DEGs, MaPNDO, MaPSAL, and MaFEDA were selected and validated using quantitative real-time PCR.
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Diachenko, Iurii,Ovchar, Oleksandr,Dubka, Oleksandr,Pastukhov, Oleksandr,Duve, Khrystyna,Kostiuk, Dmytro International Journal of Computer ScienceNetwork S 2021 International journal of computer science and netw Vol.21 No.11
The article provides an examination of those temporary states of disturbances in the psychophysiological and socio-psychological spheres of a person, which develop against the background of normally formed mechanisms of mental activity, that is, in mentally healthy people. A healthy person is a person adapted to the conditions of the external environment, and mental health is inseparable from the physical and social well-being of a person. The cause of the disorder is the impact on a person of any environmental factors that weaken the mechanisms of social and psychological adaptation of the individual.
KCI
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