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RISS 인기검색어
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- 저자 : Durga Prasad Barik (검색결과 2 건)
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Bhuyan Sudhansu Sekhar,Barik Durga Prasad,Dash Byomkesh,Rout Prachitara,Pattnaik Snigdha Samir,Verma Ramlakhan,Katara Jawahar Lal,Parameswaran C.,Devanna B. N.,Sahoo Raj Kishore,Mishra Anindita,Sabari 한국작물학회 2024 Journal of crop science and biotechnology Vol.27 No.2
Hybrid rice has a considerable yield advantage over inbred lines, but the adoption rate in India is quite slow. In a three-line hybrid rice breeding programme, the development of fertility restorer lines that have enhanced grain quality is crucial. Employment of doubled haploid (DH) technology will accelerate the development of improved R lines in a short period of time, thus hastening the pace of hybrid rice breeding. This investigation was carried out for the evaluation of genetic diversity and assessment of population differentiation in 113 DHs derived from BC1F1s of IMP. IR 42266-29-3R and Nagina 22. A total of 46 SSR (Simple Sequence Repeat) markers resulted in an average of 2.00 alleles per locus. Polymorphism information content (PIC) value ranged from 0.31 to 0.37 with an average of 0.36. RM496, RM25520, RM483 and RM553 were found to be the best markers for the identification of genetic diversity. Structure analysis classified 113 DHs into four groups, which matched the Neighbour-Joining method using UPGMA cluster analysis. The AMOVA results demonstrated substantial genetic variations within subpopulation than among sub-population. The SSR marker-based molecular fingerprinting could serve as a sound basis in the identification of genetically distant as well as in the duplicate sorting of the morphologically close population in future breeding programmes
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Biswaranjan Behera,Shashikanta Behera,Shasmita,Debasish Mohapatra,Durga Prasad Barik,Soumendra Kumar Naik 한국식물생명공학회 2021 JOURNAL OF PLANT BIOTECHNOLOGY Vol.48 No.4
Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal commu- nities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl2 (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low tem- perature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 °C; thus, they could be useful for transfer and exchange of P. foetida germplasm.
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