4-vessel occlusion으로 誘發한 흰쥐 前腦虛血의 神經細胞 損傷에 대한 大黃의 防禦效果

安德均,元道喜,金鍾虎 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

Neuroprotective and prophylatic neuroprotective effects of the Rhei Rhizoma on the rat's transient forebrain ischemia caused by 4-vessel-occlusion are concluded as following. 1. The neuroprotective effects of i.p. injection and oral administration on the neuronal density of hypothermic rats in the CA1 region of the hippocampus 7 days after 10-minute 4-BO. provided significant neuroprotection at the dose of 500 and 1000 ㎎/㎏. 2. The neuroprotective effects of Rhei Rhizoma extracts i.p. injection and oral administration on the neuronal density of normothermic rats in the CA1 region of the hippocampus 7 days after 10-minute 4-VO. provided significant neuroprotection at the dose of 500 and 1000 ㎎/㎏ against the ischemia-induced cell death when i.p injection and administration was initiated immediately after occlusion. 3. The prophylactic neuroprotective effects of Rhei Rhizoma extracts i.p. injection and oral administration pretreatment on the neuronal density of hypothermic rats in the CA1 region of the hippocampus 7 days after 10-minute 4-VO, provided significant prophylactic neuroprotection at the dosage of 500 and 1000 ㎎/㎏ against the ischemia-induced cell death when i.p. injection administration was initiated immediately after occlusion. 4. The prophylactic neuroprotective effects of Rhei Rhizoma extracts i.p. injection and oral administration pretreatment on the neuronal densith of normothermic rats in the CA1 region of the hippocampus 7 days after 10-minute 4-VO. provided significant prophylactic neuroprotection at the dosage of 1000 ㎎/㎏ against the ischemia-induced cell death when i.p. injection and administration was initiated immediately after occlution. According to the above results, Rhei Rhizoma has showed neruoprotective and prophylatic neuroprotective effects on the rats' transient forebrain ischemia caused by 4-vessel-occlusion. Therefore Rhei Rhizoma is recommended to be used for the prevention and curing of ischamia diseases.

韓國産石膏와 中國産石膏의 成分分析 및 效能比較

安德均,李尙仁 慶熙大學校 1989 論文集 Vol.18 No.-

In order to study the pharmacological effects of Gypsum, this paper deals with a comparison of the components and the contents in Korean and Chinese Cypsum, and it was studied on the laxative action of mice administered by two kinds of Cypsum. The results were as follows. 1. As the result of qualitative analysis. Fe, Mg, Mn, Ti were equal in Chinese and Korean Cypsum, but Si, Cu, Ca, Al, Sr, Cr were not equal one another. 2 As the result of quantative analysis, the contents of Mn, Ti, Cr were equal, but that of Si was remarkable in Korean Cypsum, and that of Ca was more in Chinese Cypsum. 3. In the experiment of the laxative action of mice, only drug processed Chinese Cypsum was significant. According the the above results. Korean Cypsum is able to be substituted, but not long. Therefore, it is disirbie for using drug processed Chinese Cypsum.

茵陳의 月別成分變化에 關한 硏究

李尙仁,安德均 慶熙大學校 1987 論文集 Vol.16 No.-

Arthemisiae capillaris Herba is one among those important medicine applied clinically for the treatment with hepatic disease in oriental medicine. The results quantitated capillarine, dimethvlesculetin which is known as main component of this medicine by H.P.S.C. method for the measurement of these components according to a month is that at March, April, May, June, July the author could not quantitate the amount of these components and only at August, September, October these components was acertained. Therefore it can be thought that it is recommended that Arthemisae capillaris Herba ought to be collected in autumn and to be used applied clinically.

천마 (天麻)의 4-Vessel Occlusion으로 유발한 흰쥐 전뇌허혈에 대한 신경보호 효과

金護哲,安德均 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

Gastrodiae Rhizoma(GR), the rhizoma of Gastrodia elata B₁, has been used for the effect of calming the liver(平?) in Traditional Korean Medicine. We hypothesized this effect of calming the liver has relations with the effects of curing stroke. So, this study was planned to check the neuroprotective effect of GR on the global ischemia induced by 4-vessel occlusion in Wister rats. GR extract was lyophilized with ethanol after reflux extraction with water. We induced 4-vessel occlusion for 10 minutes and reperfused again. The number of CA₁ pyramidal neruons were counted after 7 days of reperfusion under the cresyl violet staining. The result showed significant protective effects of samples compared with control group which was administrated with normal saline in the intraperitoneal injection of 1200㎎/㎏ and 600㎎/㎏ of Gastrodiae Rhizoma extracts at 0, 2 and 6 hours after inducing cerebral ischemia. We also observed the same results when it was injected at 30 minutes before inducing ischemia. The extract of GR did not lower body temperature at these dosages. Further study will be needed to separate the active ingredients of GR and study molecular biological mechanism for developing neuroprotective drug.

鹿茸이 卵巢摘出로 유발된 흰쥐의 骨多孔症에 미치는 影響

沈相度,安德均 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

The purpose of this study is to examine what are the effects of the Cervi Cornu on the aged ovariectomized rat model of postmenopausal osteoporosis. Experimantal groups(UP, MP) were administered water extract of upper portion(ash weight<25%) and middle portion(25%<ash weight<30%) of Cervi Cornu for 55 days. Control group(CO) was administered normal saline. And we estimated osteocalcin, calcium, ALP and phosphorus in serum, calcium and creatinine in urine, organ weight and changes of bone. The results of the experiment are as follows, 1. Body weight in upper portion and middle portion of Cervi Cornu group were increased in comparison with control group, but showed no efficiency. 2. The level of serum osteocalcine in upper portion of Cervi Cornu group was increased in comparison with control group, but showed no efficiency. The level of serum calcium, ALP, phosphorus, S-GOT and S-GOT in upper portion and middle portion of Cervi Cornu group showed no significant change. 3. Urinary volume in upper portion and middle portion of Cervi Cornu group showed a significant increase. The level of urine creatinine in upper portion and middle portion of Cervi Cornu group showed a significant decrease. The level of urine calcium to creatinine ratio in upper portion of Cervi Cornu showed a significant increase. 4. The weight of herat, liver, spleen and kidney in upper portion and middle portion of Cervi Cornu group showed no significant change. The weight of uterus in upper portion of Cervi Cornu group was increased in comparion with control group, but showed no efficiency. 5. The weight of tibia ash and the femur length in upper portion and middle portion of Cervi Cornu group showed a significant increase. According to the above results. effects of the Cervi Cornu on the aged ovariectomized rat model showed a efficiency in elevation of bone density by increasing bone mass and in prevention of renal function failure by ovariectomy. So Cervi Cornu can be used for prevention and curing of postmenopausal osteoporosis. As for it's effects, continuous clinical study is needed.

발산풍열약(發散風熱藥)의 항고혈압작용에 관한 연구

金護哲,朴珠瑛,安德均 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

Balsanpungyeolyak, Expelling Pathogenic Wind-Heat Drug, have been used to treat the symptoms such as fever, headache, neck stiffness and skin disturbance which are commonly shown in systemic fever disease like influenza. We thought that the symptoms from the actions of wind and heat in Traditional Korean Medicine are similar to the ones of hypertensive patients in clinic. We chose five drugs among expelling pathogenic wind-heat drugs, Puerariae Radix, Chrysanthemi Flos, Menthae Herba, Mori Folium, and Arctii Fructus, to investigate their antihypertensive effects on S.H.R. and normotensive S.D rats, direct relaxant activity on isolated rabbit aorta, and ACE inhibitory activity. Menthae Herba and Arctii Fructus decreased blood pressure significantly in SHR, but not in normotensive rats, Direct vasodilatative effects on isolated rabbit aorta was not observed in any group. Chrysanthemi Flos, Menthae Herba, Mori Folium, and Arctii Fructus inhibited the actions of ACE dose-dependently. Therefore it is concluded that expelling pathogenic wind-heat drugs has not direct correlation with antihypertensive activity. However, it can be suspected that some drugs for dispersing the actions of wind and heat may regulate blood pressure through ACE inhibition and further study will be helpful to understand the mechanism.

가시오가피의 부위별 물 추출물이 흰쥐의 혈관이완반응에 미치는 영향

姜奉錫,金亨煥,安德均,崔湖榮 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2

In this study we have examined the actions of water extract of Eleutherococus senticosus (Rupr. et Maxim.) Maxim. (ESH), Stem, Root bark (RB) and Root xylem (RX) in isolated thoracic and abdominal aorta assay. To compare the vasodilation effect, put. ESH, Stem, RB and RX into them which vessels were precontracted by 5-hydroxytryptamine (5-HT). We found out the thoracic aorta segments were responded to ESH with a dose-dependent vasodilation. The 5-HT induced contraction at 10-4M were relaxed after addition of the 2.0 ㎎/㎖ ESH as 32.1% (Stem), 34.9% (RX) and 87.2% (RB). The 5-HT induced vasocontraction at 10^-4M were vasodilated such as 87.2% in thoracic aorta and 89.0% in abdominal aorta with 2.0㎎/㎖ ESH. Addition of 2.0㎎/㎖ ESH in thoracic aorta which was precontracted by the 5-HT at 10-4M were come out differently 0% (water fraction), 53.8% (butanol fraction) and 96.6% (ethyl acetate fraction). The 5-HT induced contraction at 10-4M with and without endothelium were inhibited by 87.2% and 87.8% after addition of the ESH (2.0 ㎎/㎖). In conclusion, ESH-induced relaxation in the isolated rat thoracic aorta and abdominal aorta were come out be dose-dependent relaxation, and ESH's ethyl acetate fraction was showed the potent vasodilation effect.

大學 男女 球技選手들의 最大無機的 Power

김태운,유재충,안덕균,선우섭,이한,손두욱,박충서 慶熙大學校 社會體育硏究所 1994 體育科學論叢 Vol.- No.7

The purpose of the present research is to compare characteristics of maximal anaerobic power observed in different types of sports and player's sex differences. We have reached the following conclusions from the results we obtained by measuring maximal anaerobic power and blood lactate concentration of 60 elite university male and female players during the exercise of bicycle ergometer: 1. As to absolute and relative values of maximal anaerobic power, male players are higher in the order of rugby and handball, whereas female players are higher in the order of hockey and soccer, but no significant differences are observed between types of sports. 2. Male players are noticably higher in absolute values of maximal anaerobic power(p<0.001), but relative value is very simi1ar to that of female players. 3. It is estimated that the fact, that female players show obvious higher mean anaerobic power(p<0.001) during the 3,4KP excercise is due to sex differences in ocdurrence work load of maximal anaerobic power. 4. Comparison of blood lactate concentration at each work load in different types of sports and player's sex differences show an increase in proportion to mean anaerobic power. The results given above clearly show the differences between the type of sports such as rugby and hockey requiring power and speed, and the type of sports such as handball and soccer requiring edurance.

巴豆가 흰쥐 흉부대동맥 혈관수축에 미치는 영향

朴完洙,金亨煥,安德均,崔湖榮 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

Objectives : We have examined the vasoconstriction response to the various extracts of Crotonis Fructus (CF, Seed of Croton tiglium L.), defatted CF (DCF), roasted CF (RCF), and Croton oil in rat isolated thoracic aorta. Methods : We have examined the thoracic aorta segments obtained from rats immediately after delivery were mounted in organ baths superfused on a polygraph. Results : We found that the thoracic aorta segments responded to various extracts of CF with a dose-dependent vasoconstriction. In comparison to the 100 mM KCl induced contraction, it was contracted by 150% (water extract of CF), 144.5% (70% ethanol extract of CF), 80.1% (water extract of DCF) and 96.6% (ethanol extract of DCF) after addition of the 3 mg/ml each extract. The thoracic aorta segments were contracted by 118.5% (0.01 mg/ml Croton oil), 133.8% (3 mg/ml RCF for 10min), 123.1% (20min), 117.8% (30min), and 103.9% (40min). Conclusions : In the conclusion, CF, DCF, and RCF induced dose-dependent contraction in the isolated rat thoracic aorta. And it's major component, Croton oil, has potent vasoconstriction effect.

대승기탕(大承氣湯)의 4-VO로 유한 흰쥐뇌허혈에 대한 신경방어효과

金頀哲,安德均,金永錫 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

Daeseungkitang, the prescription which is composed of Rhei Radix, Magnoliae Cortex, Ponciri Fructus and Natrii Sulfas, has been used for the effect of expelling pathogenic heat and loosening the bowels, promoting the circulation of qi to purge accumulation in the bowels in Traditional Korean Medicine. This recipe is also one of the prescription to treat stroke in Kyung Hee University Oriental Medical Hospital. This study was planned to investigate the neuroprotective effect of Daeseungkitang on the global ischemia induced by 4-vessel occlusion in Wister rats. Daeseungkitang extract was lyophilized with ethanol after reflux extraction with water. We induced 4-vessel occlusion for 10 minutes and reperfused again. The number showed significant protective effects of samples compared with control group which was administrated with normal saline in the intraperitoneal injection of 1000 ㎎/㎏ and 500 ㎎/㎏ of Daeseungkitang extracts at 0, 2 and 6 hours after inducing cerebral ischemia.