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      • Research on Common Mode Interference of PWM Inverter Driving System

        CHENG Qiang,DU Zhong-Min 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.5

        In order to suppress electromagnetic interference of PWM driving system, electromagnetic interference characteristics ,system distribution and main influence factor in this system must be accurately mastered. Therefore this paper used conduction separation network to meter common mode interference of PWM inverter system ,and through comparison results of different conditions ,the author obtained the distribution of common mode interference on power side and load side . Then the team made analysis and improvement to the common-mode interference model, at last through theory analysis and calculation some suppression method to CM interference was found , which may provide certain theoretical guidance for EMI filter design.

      • Personal Safety in Electromagnetic Environment of Electric Vehicle

        Cheng Qiang,Du Zhong-min 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.6

        With the extensive application of automotive electrical and electronic equipment, issues of electromagnetic radiation and electromagnetic compatibility have become increasingly prominent, human body in complex automotive electromagnetic environment is bound to be some damage of electromagnetic radiation. This paper established a relatively sophisticated car simulation model,through electromagnetic characteristics of antenna model, we got automotive electric field distribution and simulation results of human body SAR value, so conclusion can be drawn that automotive electromagnetic environment is relatively safe to passengers.

      • A Novel Approach to Cloning and Expression of Human Thymidylate Synthase

        Lv, Ying-Tao,Du, Pei-Juan,Wang, Qiao-Yan,Tan, Yuan,Sun, Zong-Bin,Su, Zhong-Liang,Kang, Cong-Min Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

        Thymidylate synthase (TS) catalyzes the transfer of a methyl group from methylenetetrahydrofolate to dUMP to form dTMP. It is a primary target in the chemotherapy of colorectal cancers and some other neoplasms. In order to obtain pure protein for analysis of structure and biological function, an expression vector TS-pET28b (+) was constructed by inserting wild-type human thymidylate synthase (hTS) cDNA into pET28b (+). Then an expression strain was selected after transformation of the recombined plasmid into Rosetta (DE3). Fusion protein with His-tag was efficiently expressed in the form of inclusion bodies after IPTG induction and the content was approximately 40.0% of total bacteria proteins after optimizing expression conditions. When inclusion bodies were washed, dissolved and purified by Ni-NTA under denatured conditions, the purity was up to 90%. On SDS-PAGE and West-blotting, the protein band was found to match well with the predicted relative molecular mass-36kDa. Bioactivity was 0.1 U/mg. The results indicated that high-level expression of wild-type hTS cDNA can be achieved in prokaryotes with our novel method, facilitating research into related chemotherapy.

      • Evaluation of fruit quality in pepper germplasms(Capsicum annuum) introduced from china for high-red pigments breeding.

        Ji-Eun Hwang,Su-Gyeong Jeon,Kil-Su Jang,Oh-Hun Kwon,Tae-Young Kwon,Hongjin Long,Min Gui,Qiuyue Zhong,Lei Du 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

        Fruit quality traits like heat, color and flavor are unique and important for pepper cuisines and industrial use. Pepper pigments include chlorophyll, carotenoids, anthocyanin and red pigments like capsanthin are unique carotenoids to capsicum spp. So developing carotenoids-rich peppers and use of red pigments extraction along with capsaicinoids are one of interest pepper breeding goals in the world. Horticultural traits of 113 germplasms introduced by international cooperative research with YASS of China were evaluated to select promissing materials for high quality peppers during the past 3 years. All of germplasms including 60 local peppers were belong to C. annuum and fruit characteristics were diverse. Especially fruit chemical compositions like ASTA color, capsaicinoids and sugars were evaluated compared with korean commercial peppers. Average contents of ASTA color was 98±37, 55±63mg in capsaicinoids and 12±6% in total sugars. ASTA color which generally means red pigments content was over 120 in 25 germplasms including over 200 in 2 germplasms. Higher ASTA colors were observed in different fruit types. Capsaicinoids content was over 90mg/100g in 24 germplasms including 5 germpalsms over 200mg. Especially higher capsaicinoids were higher in local peppers with round and wrinkled fruit shape. And total sugar content was over 20% in 10 germplasms. Germplasms of different fruit shapes and characteristics will be useful materials for diverse fruit quality breeding.

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