구리와 Chromium이 초기계배에 미치는 영향

柳東錫 청주대학교 산업과학연구소 1988 産業科學硏究 Vol.6 No.-

The effects of copper and chromium on the early chick embryos were investigated. The lethal effect of chromium was higher than that of copper on the early chick embryos. Embryos treated with high doses of copper or chromium showed a high mortality when they were exposed prior to incubation. The activities of enzymes such as lactate dehydrogenase and malate dehydrogenase in embryos treated copper or chromium were much less than those of the saline treated group. But, the activity of succinate dehydrogenase was not much different from that of the saline treated group. The amounts of DNA and RNA were decreased in embryos treated with copper or chromium However, the amount of protein did not decrease as much.

Fenvalerate가 참붕어(Pseudorasbora parva)의 아가미에 미치는 영향

류동석 청주대학교 2014 産業科學硏究 Vol.31 No.2

The effects of fenvalerate on the gill of the false dace(Pseudorasbora parva) were investigated by light microscope. In the fenvalerate non-treated group and the ethanol treated group, histological changes were not observed. But, the gill in the 5㎍/ℓfenvalerate treated group showed swelling in terminal part and hyperplasia in basal part of the gill lamella. In gill lamellae in the 10㎍/ℓ fenvalerate treated group, swelling in terminal part, hyperplasia in terminal and basal part and curling were observed. In the 15㎍/ℓ fenvalerate treated group, the gill lamella showed swelling in terminal part and hyperplasia in terminal and basal part. Also curling and fusion of gill lamellae were found. It was considered that these abnormal structures of the gills of Pseudorasbora parva were affected by fenvalerate.

Angelfish(Pterophyllum eimekei)의 부화에 미치는 온도, 수소이온농도 및 염도의 영향

류동석 청주대학교 산업과학연구소 1996 産業科學硏究 Vol.14 No.-

Effects of temperature(15℃, 20℃, 25℃ and 30℃), pH value(pH 5.2, pH 6.0, pH 6.8 and pH 7.6) and salinity(0‰, 12‰, 24‰ and 36‰) on the hatching of angelfish(Pterophyllum eimekei) were investigated. The fertilized egg of angelfish was adhesive and demersal. Also, the elapsed time of hatching was 53.5 hours and the hatching rate was very high in freshwater at 30℃. The lower the water temperature was, the longer the hatching elapsed time was. At 15℃, the mortality was very high and the hatching process was highly inhibited. The hatching rate was very high in freshwater at pH 6.8 and the effect of pH was not higher than that of salinity. In salinity more than 12‰, the hatching process stopped and all embryos died out. The optimal water temperature for hatching of angelfish in freshwater was at 30℃ and the optimal pH for that was 6.8.

Zebrafish(Brachydanio rerio)의 발생에 관한 연구

류동석,김동희 청주대학교 산업과학연구소 1992 産業科學硏究 Vol.10 No.-

The development of zebrafish(Brachydanio rerio) by natural insemination was investigated. The fertilized egg of zebrafish was spherical, non-floated type with 1.12±0.04mm in diameter, and there were no oil balls and adhesive filaments in fertilized eggs. Also, the fertilized egg developed by irregular cleavage. Organogenesis was started at 12 hours and 40 minutes after fertilization. The Kupffer's vesicle and the otic vesicle were formed after the optic vesicle was formed. Also, the Kupffer's vesicle disappeared and the melanophore was formed after the eye lens was formed. At 40 hours after fertilization, the embryo with yolk hatched after the tail of embryo teared egg membrane.

버들치(Phoxinus oxycephalus) 수정란 난막의 미세구조 : Phoxinus oxycephalus

류동석 청주대학교 산업과학연구소 2004 産業科學硏究 Vol.21 No.2

The ultrastructure of the fertilized egg envelope from upstream fatminnow(Phoxinus oxycephalus) was investigated using scanning and transmission electron microscopes. An outer surface of the fertilized egg envelope was rough with round structures and small protrusions. The fertilized egg envelope consisted of three layers, an outer adhesive layer, a middle fibrillar layer and an inner lamellae layer consisting of fourteen layers In inner layer, irregular pore canals were distributed. These ultrastructural characters of fertilized egg envelope from upstream fatminnow can be utilized in taxonomy.

버들치(Phoxinus oxycephalus)의 난자형성과정

류동석 청주대학교 산업과학연구소 2002 産業科學硏究 Vol.19 No.2

The oogenesis of upstream fatminnow(Phoxinus oxycephalus) was investigated. In ovary of the upstream fatminnow, various germ cells were distributed and their shape was round. The cytoplasm of oogonia was acidic and many vesicles were located in nucleus. In primary oocytes, yolk vesicles were distributed in peripheral side of cytoplasm. Large yolk vesicles were in cytoplasm of secondary oocytes. Also, the size of oocytes is larger than that of oogonia. Yolk mass was formed and yolk vesicles were disappeared in eggs. Also the egg envelope of egg was thinner than that of other germ cells. The oogenesis of upstream fatminnow was summerized the increase of cell size, the formation and accumulation of yolk and changes of egg envelope thickness.

모래무지(Pseudogobio esocinus)의 정자형성과정

류동석 청주대학교 산업과학연구소 2006 産業科學硏究 Vol.23 No.2

The spermatogenesis of goby minnow(Pseudogobio esocinus) was investigated using transmission electron microscope. In primary spermatocytes, nuclei were round or oval and mitochondria were distributed in the cytoplasm. Secondary spermatocytes had round electron-dense nuclei and mitochondria were congregated in the side of cytoplasm. The sperm had a round head, the acrosome was not found and a motile flagellum consisted of an axoneme with a typical 9+2 pattern of microtubule.

참붕어(Pseudorasbora parva) 소장의 ALP 발현에 미치는 fenvalerate의 영향

류동석 청주대학교 2014 産業科學硏究 Vol.31 No.2

The effect of fenvalerate on ALP expression in the small intestine of Pseudorasbora parva was observed by enzyme histochemistry. In all groups, ALP was distributed in brush border in the anterior part of small intestine of Pseudorasbora parva. In 5㎍/ℓ fenvalerate treated group, ALP activity was increased than that of the fenvalerate non-treated group. But ALP activity of the 10㎍/ℓ fenvalerate treated group was decreased compared with the 5㎍/ℓ fenvalerate treated group and the fenvalerate non-treated group. In the 15㎍/ℓ fenvalerate treated group, ALP activity was increased compared with the 10㎍/ℓ fenvalerate treated group, but that was decreased compared with the fenvalerate non-treated group. It was considered that changes of ALP expression in small intestine of Pseudorasbora parva were affected by eating disorder and inflammation.

황어아과 버들치 심장의 조직학적 구조

류동석 청주대학교 2018 産業科學硏究 Vol.35 No.2

The histological structure of the heart in Rhynchocypris oxycephalus was investigated by light microscope. The heart consisted of four consecutive chambers, the sinus venosus, atrium, ventricle and bulbus arteriosus. The wall of the sinus venosus was divided into endocardium, myocardium and epicardium, and the walls of the atrium and ventricle were divided into endocardium, subendocardium, myocardium, subepicardium and epicardium, and the wall of the bulbus arteriosus was divided into endocardium, subendocardium(ridge tissue), middle layer, subepicardium and epicardium. Valves were observed in the sinoatrial, artrioventricular and bulboventricular junctions. The sinus venosus wall was mostly made up of collagen. The rings of tissue were observed at the sinoatrial junction. The atrium was composed of a spongy trabeculate myocardium surrounded by an external rim of thin myocardium, and collagens were distributed in the subepicardium and trabeculae. The ventricular myocardium was an entirely spongy myocardium with vessels in subepicardium. In the subepicardium and trabeculae of the ventricle, collagens were distributed. Collagens and elastins were demonstrated in the bulbus arteriosus, but elastins were excepted in the subepicardium.

밀어(Rhinogobius brunneus)의 정자형성과정

류동석 청주대학교 산업과학연구소 2000 産業科學硏究 Vol.17 No.2

The spermatogenesis of common freshwater goby(Rhinogobius brunneus) was investigated. The testis of common freshwater goby consisted of numerous testicular sacs contained synchronized cells. Leydig cells were located among testicular sacs. In primary spematocytes, nuclei were round and mitochondria were distributed in the cytoplasm. The nucleus of secondary spermatocyte was oval and mitochondria were congregated in the side of cytoplasm. The spermatid had oval and electron dense nucleus, mitochondria in the cytoplasm were disappeared and a base of flagellum was appeared. The sperm had a elongated conical head, the acrosome was not found and a motile flagellum consisted of an axoneme with a typical 9+2 pattern of microtubule.