http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Kartika Sari Dewi ),( Ratna Annisa Utami ),( Hariyatun Hariyatun ),( Riyona Desvy Pratiwi ),( Dian Fitria Agustiyanti ),( Asrul Muhamad Fuad ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.2
Single-chain antibodies against epidermal growth factor receptor variant III (EGFRvIII) are potentially promising agents for developing antibody-based cancer treatment strategies. We described in our previous study the successful expression of an anti-EGFRvIII scFv antibody in Escherichia coli. However, we could also observe the formation of insoluble aggregates in the periplasmic space, limiting the production yield of the active product. In the present study, we investigated the mechanisms by which growth conditions could affect the expression of the soluble anti-EGFRvIII scFv antibody in small-scale E. coli NiCo21(DE3) cultures, attempting to maximize production. The secreted scFv molecules were purified using Ni-NTA magnetic beads and protein characterization was performed using SDS-PAGE and western blot analyses. We used the ImageJ software for protein quantification and determined the antigen-binding activity of the scFv antibody against the EGFRvIII protein. Our results showed that the highest percentage of soluble scFv expression could be achieved under culture conditions that combined low IPTG concentration (0.1 mM), low growth temperature (18℃), and large culture dish surface area. We found moderate-yield soluble scFv production in the culture medium after lactose-mediated induction, which was also beneficial for downstream protein processing. These findings were confirmed by conducting western blot analysis, indicating that the soluble, approximately 30-kDa scFv molecule was localized in the periplasm and the extracellular space. Moreover, the antigen-binding assay confirmed the scFv affinity against the EGFRvIII antigen. In conclusion, our study reveals that low-speed protein expression is preferable to obtain more soluble anti-EGFRvIII scFv protein in an E. coli expression system.
Kurniawati Hastuti DEWI,Sandy Nur Ikfal RAHARJO,DESMIWATI,Kresno Agus HENDARTO,Aam AMINAH,Tri Astuti WISUDAYATI,Hasan ROYANI,Anggi Dian Safitri HASIBUAN,Dian Ratna SARI Asian Center for Women's Studies : Ewha Womans Uni 2020 Asian Journal of Women's Studies(AJWS) Vol.26 No.4
Using the Harvard Analytical Framework (HAF), we analyze the role of farmers (male and female) in the Special Purpose Forest Area of Parungpanjang, Indonesia. Primary data for this study were collected through interviews and focus group discussions with male and female farmers at the research site in September 2019. This shows that female farmers participate in all dimensions of productive, reproductive, and socio-political activities, including aspects of control, while male farmers tend to limit their participation only to productive and socio-political activities. It confirms the findings of earlier research that there is no significant gender difference between the roles of female and male farmers in terms of natural resource management and control in Southeast Asia. However, one problem remains: the Special Purpose Forest Area of Parungpanjang has not given official registration rights to female farmers. By presenting their voices, we suggest the need for the strengthening of gender-responsive policies such as granting registration rights to female farmers to access and use land and conduct gender awareness programs among male farmers. Dengan menggunakan Kerangka Analisis Harvard, kami menganalisis peran para petani (laki laki dan perempuan) di Kawasan Hutan dengan Tujuan Khusus Parungpanjang, Indonesia. Data primer dikumpulkan melalui wawancara dan diskusi kelompok terpumpun dengan para petani laki-laki dan perempuan di lokasi penelitian pada September 2019. Kajian ini menunjukkan bahwa petani perempuan berpartisipasi pada semua dimensi kegiatan produktif, reproduktif, dan sosio-politik, termasuk juga aspek kontrol, sementara petani laki-laki cenderung membatasi partisipasi mereka pada kegiatan produktif dan sosio-politik saja. Hal ini mengonfirmasi temuan penelitian-penelitian sebelumnya bahwa tidak ada perbedaan peran gender yang signifikan antara petani laki-laki dan perempuan dalam pengelolaan sumber daya alam di Asia Tenggara. Meskipun demikian, masih ada masalah yaitu Kawasan Hutan dengan Tujuan Khusus Parungpanjang belum memberikan hak pendaftaran kepada para petani perempuan. Dengan menyajikan suara para petani perempuan, kajian ini mendorong penguatan kebijakan responsif gender seperti pemberian hak pendaftaran kepada para petani perempuan untuk mengakses dan mengelola lahan, serta pelaksanaan program penyadaran gender untuk para petani laki-laki.