Enhanced Recovery after Surgery for Gastric Cancer Patients Improves Clinical Outcomes at a US Cancer Center

Desiderio, Jacopo,Stewart, Camille L.,Sun, Virginia,Melstrom, Laleh,Warner, Susanne,Lee, Byrne,Schoellhammer, Hans F.,Trisal, Vijay,Paz, Benjamin,Fong, Yuman,Woo, Yanghee The Korean Gastric Cancer Association 2018 Journal of gastric cancer Vol.18 No.3

Purpose: Enhanced recovery after surgery (ERAS) protocols for gastric cancer patients have shown improved outcomes in Asia. However, data on gastric cancer ERAS (GCERAS) programs in the United States are sparse. The purpose of this study was to compare perioperative outcomes before and after implementation of an GC-ERAS protocol at a National Comprehensive Cancer Center in the United States. Materials and Methods: We reviewed medical records of patients surgically treated for gastric cancer with curative intent from January 2012 to October 2016 and compared the GC-ERAS group (November 1, 2015-October 1, 2016) with the historical control (HC) group (January 1, 2012-October 31, 2015). Propensity score matching was used to adjust for age, sex, number of comorbidities, body mass index, stage of disease, and distal versus total gastrectomy. Results: Of a total of 95 identified patients, matching analysis resulted in 20 and 40 patients in the GC-ERAS and HC groups, respectively. Lower rates of nasogastric tube (35% vs. 100%, P<0.001) and intraabdominal drain placement (25% vs. 85%, P<0.001), faster advancement of diet (P<0.001), and shorter length of hospital stay (5.5 vs. 7.8 days, P=0.01) were observed in the GC-ERAS group than in the HC group. The GC-ERAS group showed a trend toward increased use of minimally invasive surgery (P=0.06). There were similar complication and 30-day readmission rates between the two groups (P=0.57 and P=0.66, respectively). Conclusions: The implementation of a GC-ERAS protocol significantly improved perioperative outcomes in a western cancer center. This finding warrants further prospective investigation.

Atomized Nasal Douche:Our Experience

Desiderio Passàli,Chiara Mezzedimi,Giulio C. Passàli,Francesco M. Passàli,Luisa Bellussi 대한비과학회 2001 Journal of rhinology Vol.8 No.1

Background and Objectives:A recently proposed apparatus for the treatment of nasal inflammatory affections is theatomized nasal douche. The aim of the paper was to assess the efficacy of the atomized nasal douche in patients affected byacute or chronic rhinopathy. Materials and Methods:We selected 60 adult patients:They were subdivided into twogroups and each group consisted of 15 patients suffering from vasomotor rhinitis and 15 patients affected by acute rhinitis. The former underwent atomized nasal douche and the latter underwent nasal lavages with saline solution. We performedbacterial cultures of the nasal swab and the secretions drawn from the nasal cavity, a rhinomanometric test, an assessment ofthe symptomatologic score before and after the treatment in all the patients. Results:At the end of treatment, there was areduction or complete disappearance of the bacterial colonies in 23 of the 30 patients treated with the atomized nasaldouche, while in the control group the same result occurred in only 10 of the 30 patients. Conclusions:Our results confirm thatthe atomized nasal douche performs an efficacious lavage of the nasal cavity, representing a good support for thetreatment of inflammatory processes of the nose.

Enhanced Recovery after Surgery for Gastric Cancer Patients Improves Clinical Outcomes at a US Cancer Center

Jacopo Desiderio,Camille L. Stewart,Virginia Sun,Laleh Melstrom,Susanne Warner,Byrne Lee,Hans F. Schoellhammer,Vijay Trisal,Benjamin Paz,Yuman Fong,우양희 대한위암학회 2018 Journal of gastric cancer Vol.18 No.3

Purpose: Enhanced recovery after surgery (ERAS) protocols for gastric cancer patients have shown improved outcomes in Asia. However, data on gastric cancer ERAS (GC-ERAS) programs in the United States are sparse. The purpose of this study was to compare perioperative outcomes before and after implementation of an GC-ERAS protocol at a National Comprehensive Cancer Center in the United States. Materials and Methods: We reviewed medical records of patients surgically treated for gastric cancer with curative intent from January 2012 to October 2016 and compared the GC-ERAS group (November 1, 2015–October 1, 2016) with the historical control (HC) group (January 1, 2012–October 31, 2015). Propensity score matching was used to adjust for age, sex, number of comorbidities, body mass index, stage of disease, and distal versus total gastrectomy. Results: Of a total of 95 identified patients, matching analysis resulted in 20 and 40 patients in the GC-ERAS and HC groups, respectively. Lower rates of nasogastric tube (35% vs. 100%, P<0.001) and intraabdominal drain placement (25% vs. 85%, P<0.001), faster advancement of diet (P<0.001), and shorter length of hospital stay (5.5 vs. 7.8 days, P=0.01) were observed in the GC-ERAS group than in the HC group. The GC-ERAS group showed a trend toward increased use of minimally invasive surgery (P=0.06). There were similar complication and 30-day readmission rates between the two groups (P=0.57 and P=0.66, respectively). Conclusions: The implementation of a GC-ERAS protocol significantly improved perioperative outcomes in a western cancer center. This finding warrants further prospective investigation.

The SAR92 Clade: an Abundant Coastal Clade of Culturable Marine Bacteria Possessing Proteorhodopsin

Stingl, Ulrich,Desiderio, Russell A.,Cho, Jang-Cheon,Vergin, Kevin L.,Giovannoni, Stephen J. American Society for Microbiology 2007 Applied and environmental microbiology Vol.73 No.7

<B>ABSTRACT</B><P>Proteorhodopsin (PR) is a protein that is abundant in marine bacterioplankton. PR is hypothesized to be a light-dependent proton pump, thus creating a proton gradient that can be used for energy production without electron transport. Currently, the only culture that has been reported to possesses PR is the highly abundant alphaproteobacterium “<I>Candidatus</I> Pelagibacter ubique” (SAR11 clade), but surprisingly, its growth in batch culture was not enhanced by light. Here, we present the first cultured gammaproteobacterium that possesses a PR gene. Genome sequencing and analysis of HTCC2207 showed that the PR gene is present as a lone transcriptional unit directly followed by an operon containing genes that are presumably involved in the synthesis of retinal, the chromophore of PR. Half-time decay times of different PR intermediates in native HTCC2207 cells ranged between 2 and 15 ms, and the absorbance maximum of PR was determined to be 528 nm. Proteorhodopsin was identified in three additional strains, using a specific PCR assay on other cultured members of the SAR92 clade. Phylogenetic analyses of the PR genes determined that they form a deeply rooting cluster not closely related to any PR genes recovered so far. Fluorescence in situ hybridization and RNA blots showed that the SAR92 clade reaches up to 10% of the total bacterial population in surface waters close to the Oregon coast and decreases over depth and distance from the shore. Although the growth of HTCC2207 is limited by the amount of available carbon that is present in the medium applied, these cultures do not grow at higher rates nor do they have higher growth yields when incubated under light.</P>

hCTR9, a Component of Paf1 Complex, Participates in the Transcription of Interleukin 6-responsive Genes through Regulation of STAT3-DNA Interactions

Youn, Min-Young,Yoo, Hyun-Seok,Kim, Min-Jung,Hwang, Sun-Young,Choi, Yongwook,Desiderio, Stephen V.,Yoo, Joo-Yeon American Society for Biochemistry and Molecular Bi 2007 The Journal of biological chemistry Vol.282 No.48

Gram-positive bacteria produce membrane vesicles: Proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles

Lee, Eun-Young,Choi, Do-Young,Kim, Dae-Kyum,Kim, Jung-Wook,Park, Jung Ok,Kim, Sungjee,Kim, Sang-Hyun,Desiderio, Dominic M.,Kim, Yoon-Keun,Kim, Kwang-Pyo,Gho, Yong Song WILEY-VCH Verlag 2009 Proteomics Vol.9 No.24

<P>Although archaea, Gram-negative bacteria, and mammalian cells constitutively secrete membrane vesicles (MVs) as a mechanism for cell-free intercellular communication, this cellular process has been overlooked in Gram-positive bacteria. Here, we found for the first time that Gram-positive bacteria naturally produce MVs into the extracellular milieu. Further characterizations showed that the density and size of Staphylococcus aureus-derived MVs are both similar to those of Gram-negative bacteria. With a proteomics approach, we identified with high confidence a total of 90 protein components of S. aureus-derived MVs. In the group of identified proteins, the highly enriched extracellular proteins suggested that a specific sorting mechanism for vesicular proteins exists. We also identified proteins that facilitate the transfer of proteins to other bacteria, as well to eliminate competing organisms, antibiotic resistance, pathological functions in systemic infections, and MV biogenesis. Taken together, these observations suggest that the secretion of MVs is an evolutionally conserved, universal process that occurs from simple organisms to complex multicellular organisms. This information will help us not only to elucidate the biogenesis and functions of MVs, but also to develop therapeutic tools for vaccines, diagnosis, and antibiotics effective against pathogenic strains of Gram-positive bacteria.</P>