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油菜의 葯培養 硏究 Ⅰ. 基本培地 選定 및 葯의 適正 置床時期 究明
Jae Gyun Gwag(郭在均),Gun Sik Chung(鄭根植),Hyung Soo Suh(徐亨洙),Deog Sueng Lee(李德承),Bong Ho Lee(李奉鎬) 한국육종학회 1987 한국육종학회지 Vol.19 No.4
This study was conducted to confirm the effect of basal medium and to determine the adequate anther stage in anther culture of Brassica napus. Embryos were obtained only from Keller et al. medium and were detected in responding anthers after 13 days of culture and virtually completed by the day 38. The range of developmental stages varying from globular types to mature forms with developed cotyledons was observed. Microspore-derived haploids were characterized by slender flower bud and small flower size with poorly developed anther. Bud in which petal length varies from one-fourth to one-half of the length of the anther contained pollen was detected as the optimal culture stage.