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      저자 : Debajyoti Goswami (검색결과 2 건)
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        Erucic Acid Production Using Porcine Pancreas Lipase: Enhancement by Mixed Surfactants

        Debajyoti Goswami,Jayanta Kumar Basu,Sirshendu De 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.2

        Application of mixed surfactants coupled with statistical optimization in lipase catalyzed oil hydrolysis is presented for the first time in this study. Selective hydrolysis of brown mustard oil to erucic acid by porcine pancreas lipase was enhanced by mixed surfactants comprising of an oil-soluble nonionic surfactant (Span 80) and a watersoluble nonionic surfactant (Tween 80). The production of erucic acid was maximized using statistically designed experiments and subsequent analysis of their result by response surface methodology. The most significant variables were enzyme concentration and concentration of Tween 80. Small changes in pH and concentration of Span 80 also produced a significant change in the production of erucic acid. Temperature and speed of agitation were insignificant variables and were fixed at 35℃ and 900 rpm, respectively. Under these conditions, the optimal combination of other variables were pH 9.65, 2.13 mg/g enzyme in oil, 9.8× 10^(−3) M Span 80 (in oil), and 4 × 10^(−3) M Tween 80 (in buffer). These conditions led to formation of 99.69% of the total erucic acid in 1.25 h. Interaction of enzyme concentration with pH significantly affected erucic acid production.

      • KCI등재

        Optimization of Process Variables in Castor Oil Hydrolysis by Candida Rugosa Lipase with Buffer as Dispersion Medium

        Debajyoti Goswami,Jayanta Kumar Basu,Sirshendu De 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.2

        In this study, castor oil is hydrolyzed in presence of Candida rugosa lipase, while in the buffer (aqueous) phase as a dispersion medium. The following conditions were used to optimize the process: speed of agitation, initial pH of buffer phase, temperature, and ratio of buffer phase volume to oil weight. The optimal conditions are 1,100 rpm, pH 6.5, temperature 35°C, and 3:1 buffer phase volume to oil weight ratio. Under these described conditions, the reusability of lipase was tested and it was found that nearly 80% of original hydrolysis percentage was achieved after the first recycle In this study, castor oil is hydrolyzed in presence of Candida rugosa lipase, while in the buffer (aqueous) phase as a dispersion medium. The following conditions were used to optimize the process: speed of agitation, initial pH of buffer phase, temperature, and ratio of buffer phase volume to oil weight. The optimal conditions are 1,100 rpm, pH 6.5, temperature 35°C, and 3:1 buffer phase volume to oil weight ratio. Under these described conditions, the reusability of lipase was tested and it was found that nearly 80% of original hydrolysis percentage was achieved after the first recycle

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