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( Dao Jing Xu ),( Ying Ze Zhao ),( Jin Wang ),( Juan Wen He ),( Ya Guang Weng ),( Jin Yong Luo ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.4
Although previous studies have demonstrated that BMP9 is highly capable of inducing osteogenic differentiation of mesenchymal stem cells, the molecular mechanism involved remains to be fully elucidated. In this study, we showed that BMP9 simultaneously promotes the activation of Smad1/5/8, p38 and ERK1/2 in C3H10T1/2 cells. Knockdown of Smad4 with RNA interference reduced nuclear translocation of Smad1/5/8, and disrupted BMP9-induced osteogenic differentiation. BMP9-induced osteogenic differentiation was blocked by p38 inhibitor SB203580, whereas enhanced by ERK1/2 inhibitor PD98059. SB203580 decreased BMP9-activated Smads singling, and yet PD98059 stimulated Smads singling in C3H10T1/2 cells. The effects of inhibitor were reproduced with adenovirus expressing siRNA targeted p38 and ERK1/2, respectively. Taken together, our findings revealed that Smads, p38 and ERK1/2 are involved in BMP9-induced osteogenic differentiation. Also, it is noteworthy that p38 and ERK1/2 may play opposing regulatory roles in mediating BMP9-induced osteogenic differentiation of C3H10T1/2 cells. [BMB reports 2012; 45(4): 247-252]
Y. C. Lin,Hui Yang,Dong‑Dong Chen,Dao‑Guang He 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.2
The intermediate-temperature (473–973 K) deformation behavior of a nickel-based superalloy is researched by uniaxialtensile experiments. It is observed that the flow characteristics and the Portevin–Le Chatelier (PLC) effects are significantlyaffected by the thermo-mechanical parameters. The serrated flow features are obvious under the testing conditions. A stackedauto-encoders (SAEs) network model is proposed for predicting the flow behaviors of the researched superalloy. The architectureof the established SAEs model is optimized layer by layer. The best number of hidden layer is 3, and the nodes perhidden layer are 15, 20 and 50, respectively. The excellent prediction ability suggests that the developed SAEs model canwell reconstruct the intermediate-temperature flow behavior involving the PLC effects of the researched superalloy.