AIMP1/p43 protein induces the maturation of bone marrow-derived dendritic cells with T helper type 1-polarizing ability.

Kim, Eugene,Kim, Seung Hyun,Kim, Sunghoon,Cho, Daeho,Kim, Tae Sung Williams Wilkins 2008 JOURNAL OF IMMUNOLOGY Vol.180 No.5

<P>AIMP1 (ARS-interacting multifunctional protein 1), previously known as p43, was initially identified as a factor associated with a macromolecular tRNA synthetase complex. Recently, we demonstrated that AIMP1 is also secreted and acts as a novel pleiotropic cytokine. In this study, we investigated whether AIMP1 induces the activation and maturation of murine bone marrow-derived dendritic cells (DCs). AIMP1-treated DCs exhibited up-regulated expression of cell-surface molecules, including CD40, CD86, and MHC class II. Additionally, microarray analysis and RT-PCR determinations indicated that the expression of known DC maturation genes also increased significantly following treatment with AIMP1. Treatment of DCs with AIMP1 resulted in a significant increase in IL-12 production and Ag-presenting capability, and it also stimulated the proliferation of allogeneic T cells. Importantly, AIMP1-treated DCs induced activation of Ag-specific Th type 1 (Th1) cells in vitro and in vivo. AIMP1-stimulated DCs significantly enhanced the IFN-gamma production of cocultured CD4+ T cells. Immunization of mice with keyhole limpet hemocyanin-pulsed AIMP1 DCs efficiently led to Ag-specific Th1 cell responses, as determined by flow cytometry and ELISA. The addition of a neutralizing anti-IL-12 mAb to the cell cultures that had been treated with AIMP1 resulted in the decreased production of IFN-gamma, thereby indicating that AIMP1-stimulated DCs may enhance the Th1 response through increased production of IL-12 by APCs. Taken together, these results indicate that AIMP1 protein induces the maturation and activation of DCs, which skew the immune response toward a Th1 response.</P>

Enhanced S100A4 protein expression is clinicopathologically significant to metastatic potential and p53 dysfunction in colorectal cancer

Kim, Joo Heon,Kim, Chang Nam,Kim, Soo Young,Lee, Jung Sam,Cho, Daeho,Kim, Jae Wha,Yoon, Sun Young Spandidos Publications 2009 ONCOLOGY REPORTS Vol.22 No.1

<P>To investigate the expression levels of S100A4 in human colorectal carcinoma (CC) and its relationship with clinicopathological parameters and metastatic potential, 73 pathological specimens from patients with CC were examined for S100A4 expression by RT-PCR and immunohistochemistry. An increase of S100A4 mRNA was observed in 19/23 (82.6%) CC specimens, and S100A4 was up-regulated in 40/73 (54.7%) CC cases compared with non-neoplastic mucosal tissues. Upregulation of S100A4 was significantly related to invasion, nodal status, distant metastasis and p53 expression. Next, we investigated whether S100A4 could affect p53 transactivation and stability. Interestingly, it was revealed that treatment with exogenous S100A4 protein reduced transcriptional activity of p53 and abrogated the modification of calcium binding affinity of S100A4 protein. These findings suggested that S100A4 might be involved in the progression and metastasis of human CC, presumably via modulation of the wild-type p53 protein.</P>

Memory Characteristics Of Silicon Nanocrystal Dot Memory Device With Er-Silicide Source/Drain Structure

Daeho Son,Eunkyeom Kim,Kyongmin Kim,Jungho Kim,Kyungsu Lee,Sunghwan Won,Junghyun Sok,Wan-Shick Hong,Kyoungwan Park,Taeyoub Kim,Moongyu Jang 한국진공학회 2008 한국진공학회 학술발표회초록집 Vol.2008 No.2

Microwave-Assisted Protein Digestion in a Plate Well for Facile Sampling and Rapid Digestion

Kim, Hyeonil,Kim, Han Sol,Lee, Dabin,Shin, Dongwon,Shin, Daeho,Kim, Jeongkwon,Kim, Jungbae American Chemical Society 2017 ANALYTICAL CHEMISTRY - Vol.89 No.20

<P>Protein digestion is one of the most important processes in proteomic analysis. Here, we report microwave-assisted protein digestion in a plate well, which allows for facile sampling as well as rapid protein digestion based on the combination of highly stable enzyme immobilization and 3D printing technologies. Trypsin (TR) was immobilized on polystyrene-based nanofibers via an enzyme coating (EC) approach. The EC with stabilized TR activity was assembled with the 3D-printed structure in the plate well (EC/3D), which provides two separated compartments for the solution sampling and the TR-catalyzed protein digestion, respectively. EC/3D can effectively prevent the interference of sampling by accommodating EC in the separated compartment from the sampling hole in the middle. EC/3D in the plate well maintained its protein digestion performance under shaking over 160 days. Microwave irradiation enabled the digestion of bovine serum albumin within 10 min, generating the MALDI-TOF MS results of 75.0% sequence coverage and 61 identified peptides. EC/3D maintained its protein digestion performance under microwave irradiation after 30 times of recycled uses. EC/3D in the plate well has demonstrated its potential as a robust and facile tool for the development of an automated protein digestion platform. The combination of stable immobilized enzymes and 3D-printed structures can be potentially utilized not only for the protein digestion, but also for many other enzyme applications, including bioconversion and biosensors.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2017/ancham.2017.89.issue-20/acs.analchem.7b02169/production/images/medium/ac-2017-02169j_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac7b02169'>ACS Electronic Supporting Info</A></P>

Psychometric Validation of the Korean Version of Structured Interview for Post-traumatic Stress Disorder (K-SIP)

Kim, Won,Kim, Daeho,Seo, Ho-Jun,Lee, Sang-Yeol,Ryu, Seung-Ho,Kim, Jung-Bum,Chung, Moon Yong,Koo, Young Jin,Ryu, Seong Gon,Kim, Eui Jung,Kim, Tae-Suk,Lim, Hyun-Kook,Woo, Jong-Min,Chae, Jeong-Ho The Korean Academy of Medical Sciences 2009 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.24 No.1

<P>For diagnosis and management of post-traumatic stress disorder (PTSD), the easily administered assessment tool is essential. Structured Interview for PTSD (SIP) is a validated, 17-item, simple measurement being used widely. We aimed to develop the Korean version of SIP (K-SIP) and investigated its psychometric properties. Ninety-three subjects with PTSD, 73 subjects with mood disorder or anxiety disorder as a psychiatric control group, and 88 subjects as a healthy control group were enrolled in this study. All subjects completed psychometric assessments that included the K-SIP, the Korean versions of the Clinician-Administered PTSD Scale (CAPS) and other assessment tools. The K-SIP presented good internal consistency (Cronbach's α=0.92) and test-retest reliability (r=0.87). K-SIP showed strong correlations with CAPS (r=0.72). Among three groups including PTSD patients, psychiatric controls, and normal controls, there were significant differences in the K-SIP total score. The potential cut-off total score of K-SIP was 20 with highest diagnostic efficiency (91.9%). At this point, the sensitivity and specificity were 95.5% and 88.4%, respectively. Our result showed that K-SIP had good reliability and validity. We expect that K-SIP will be used as a simple but structured instrument for assessment of PTSD.</P>

NDRG2 expression decreases with tumor stages and regulates TCF/β-catenin signaling in human colon carcinoma

Kim, Young-Jun,Yoon, Sun Y.,Kim, Jong-Tae,Song, Eun Y.,Lee, Hee G.,Son, Hyun J.,Kim, Soo Y.,Cho, Daeho,Choi, Inpyo,Kim, Joo H.,Kim, Jae W. Oxford University Press 2009 Carcinogenesis Vol.30 No.4

<P>NDRG (N-Myc downstream-regulated gene)-2 is a member of the NDRG family. Although it has been suggested that NDRG2 is involved in cellular differentiation and tumor suppression, its intracellular signal and regulatory mechanism are not well known. Here, we show the differential expression of NDRG2 in human colon carcinoma cell lines and tissues by reverse transcription–polymerase chain reaction and immunohistochemical analyses with monoclonal antibody against NDRG2. NDRG2 was strongly expressed in normal colonic mucosa and colonic adenomatous tissues (25 of 25) but not in all invasive cancer tissues [44 of 99 (44%)]. Most distinctive results indicated that the high expression level of NDRG2 has a positive correlation with tumor differentiation and inverse correlation with tumor invasion depth and Dukes’ stage of colon adenocarcinoma. To investigate the roles of NDRG2 in tumorigenesis, we used <I>in vitro</I> cell culture system. SW620 colon cancer cell line with a low level of intrinsic NDRG2 protein was transfected with <I>NDRG2</I>-expressing plasmid. TOPflash luciferase reporter assay showed that the transcriptional activity of T-cell factor (TCF)/lymphoid enhancer factor (LEF) was reduced by NDRG2 introduction, but not by the introduction of mutant NDRG2 generated by deletion or site-directed mutagenesis. Intracellular β-catenin levels were slightly reduced in the NDRG2-transfected SW620 cells and this regulation of β-catenin stability and TCF/LEF activity were mediated through the modulation of glycogen synthase kinase-3beta activity by NDRG2 function. Our results suggest that NDRG2 might play a pivotal role as a potent tumor suppressor by the attenuation of TCF/β-catenin signaling for the maintenance of healthy colon tissues.</P>

Metagenomic Approach to Identifying Foodborne Pathogens on Chinese Cabbage

( Daeho Kim ),( Sanghyun Hong ),( You-tae Kim ),( Sangryeol Ryu ),( Hyeun Bum Kim ),( Ju-hoon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.2

Foodborne illness represents a major threat to public health and is frequently attributed to pathogenic microorganisms on fresh produce. Recurrent outbreaks often come from vegetables that are grown close to or within the ground. Therefore, the first step to understanding the public health risk of microorganisms on fresh vegetables is to identify and describe microbial communities. We investigated the phyllospheres on Chinese cabbage (Brassica rapa subsp. pekinensis, N = 54). 16S rRNA gene amplicon sequencing targeting the V5- V6 region of 16S rRNA genes was conducted by employing the Illumina MiSeq system. Sequence quality was assessed, and phylogenetic assessments were performed using the RDP classifier implemented in QIIME with a bootstrap cutoff of 80%. Principal coordinate analysis was performed using a weighted Fast UniFrac matrix. The average number of sequence reads generated per sample was 34,584. At the phylum level, bacterial communities were composed primarily of Proteobacteria and Bacteroidetes. The most abundant genera on Chinese cabbages were Chryseobacterium, Aurantimonadaceae_g, Sphingomonas, and Pseudomonas. Diverse potential pathogens, such as Pantoea, Erwinia, Klebsiella, Yersinia, Bacillus, Staphylococcus, Salmonella, and Clostridium were also detected from the samples. Although further epidemiological studies will be required to determine whether the detected potential pathogens are associated with foodborne illness, our results imply that a metagenomic approach can be used to detect pathogenic bacteria on fresh vegetables.

Erdr1 Attenuates Dermatophagoides farina Body Extract-Induced Atopic Dermatitis in NC/Nga Mice

Kim, Kyung Eun,Jung, Myung Jin,Houh, Younkyung,Kim, Tae Sung,Lee, Wang Jae,Yang, Yoolhee,Bang, Sa Ik,Kim, Chang Han,Kim, Heejong,Park, Hyun Jeong,Cho, Daeho Williams & Wilkins 2017 The Journal of investigative dermatology Vol.137 No.8

Dither-Frequency Tuning Technique for RSOA-Based Coherent WDM PON

Kim, Daeho,Kim, Byung Gon,Bo, Tianwai,Kim, Hoon IEEE 2019 IEEE photonics technology letters Vol.31 No.1

<P>Increasing the transmission distance is an attractive technological option for lowering the cost of loopback-configured coherent wavelength-division-multiplexed passive optical networks implemented by using reflective semiconductor optical amplifiers (RSOAs). In this regard, it is necessary to increase the fiber launch power of the seed light provided from the central office. However, the stimulated Brillouin scattering (SBS) limits this power to less than 6 dBm. It is well-known that the frequency-dithering technique increases the SBS threshold effectively, allowing us to launch a higher-power continuous-wave light. However, the linewidth of the seed laser (caused by the frequency dither) makes the carrier phase estimation (CPE) very difficult at the coherent receiver. To solve this problem, we propose a dither-frequency tuning technique in this letter. We set the dither frequency to be the integer times the inverse of the round-trip propagation delay. Then, we can make the frequency dither of the upstream signal unseen at the receiver since it becomes in phase with the frequency dither of the local oscillator. Thus, the CPE performance would not be limited by the frequency dither, but by the natural linewidth of the seed laser. The efficacy of the proposed technique is experimentally demonstrated over a 60-km loopback link using a 28-Gb/s quadrature phase-shift keying signal generated from RSOA.</P>

Analysis of Microbiota in Bellflower Root, Platycodon grandiflorum, Obtained from South Korea

( Daeho Kim ),( Sanghyun Hong ),( Hongjun Na ),( Jihwan Chun ),( Robin B. Guevarra ),( You-tae Kim ),( Sangryeol Ryu ),( Hyeun Bum Kim ),( Ju-hoon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.4

Bellflower root (Platycodon grandiflorum), which belongs to the Campanulaceae family, is a perennial grass that grows naturally in Korea, northeastern China, and Japan. Bellflower is widely consumed as both food and medicine owing to its high nutritional value and potential therapeutic effects. Since foodborne disease outbreaks often come from vegetables, understanding the public health risk of microorganisms on fresh vegetables is pivotal to predict and prevent foodborne disease outbreaks. We investigated the microbial communities on the bellflower root (n = 10). 16S rRNA gene amplicon sequencing targeting the V6-V9 regions of 16S rRNA genes was conducted via the 454-Titanium platform. The sequence quality was checked and phylogenetic assessments were performed using the RDP classifier implemented in QIIME with a bootstrap cutoff of 80%. Principal coordinate analysis was performed using the weighted Fast UniFrac distance. The average number of sequence reads generated per sample was 67,192 sequences. At the phylum level, bacterial communities from the bellflower root were composed primarily of Proteobacteria, Firmicutes, and Actinobacteria in March and September samples. Genera Serratia, Pseudomonas, and Pantoea comprised more than 54% of the total bellflower root bacteria. Principal coordinate analysis plots demonstrated that the microbial community of bellflower root in March samples was different from those in September samples. Potential pathogenic genera, such as Pantoea, were detected in bellflower root samples. Even though further studies will be required to determine if these species are associated with foodborne illness, our results indicate that the 16S rRNA gene-based sequencing approach can be used to detect pathogenic bacteria on fresh vegetables.