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- 저자 : Cong Danh Nguyen (검색결과 3 건)
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[ICACE2022] Model identification of two double-acting pistons pump
Danh Khoa Nguyen,Cong Toai Truong,Van Tu Duong,Huy Hung Nguyen,Tan Tien Nguyen 한국마린엔지니어링학회 2023 한국마린엔지니어링학회지 Vol.47 No.2
The Covid-19 pandemic has had a comprehensive and far-reaching impact on various fields, especially in the medical aspect. Additionally, many variants of this coronavirus have appeared due to mutation, each of which has distinguished influences. During the Covid-19 pandemic, medical equipment, especially ventilators, has seen a severe shortage. Thus, many studies of ventilators machine have been carried out around the world. And according to these studies, airflow control is one of the critical issues of ventilator machine research that saves human life. Understanding the above problem, it is necessary to conduct system modeling and obtain a model which has high linearity so that the parameters of the ventilation can be controlled to achieve better accuracy. This paper presents the model identification of a two double-acting piston pump system using experimental data. First order plus dead time method is applied to achieve the identification model. The data used for identification and validation are collected by conducting several practical tests on the two double-acting pistons pump system. The discussion on the identified model and future works are also included in this paper.
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Genetic complementation analysis of rice sucrose transporter genes in Arabidopsis SUC2 mutant atsuc2
엄준섭,Cong Danh Nguyen,이대우,이상규,전종성 한국식물학회 2016 Journal of Plant Biology Vol.59 No.3
Sucrose transporters (SUTs) play a critical role on the phloem plasma membrane in loading sucrose into the phloem of source leaves for long-distance transport to sink organs. Rice has a small gene family of five SUTs, Oryza sativa SUT1 (OsSUT1) to OsSUT5. To identify rice SUTs that function as phloem loaders, we adopted a growth restoration assay of the severe growth retardation phenotype of atsuc2, a mutant of the best-characterized Arabidopsis phloem loader AtSUC2, by introducing OsSUTs. The rice SUT genes were expressed by two different promoters, the native phloem-specific promoter of AtSUC2 (pAtSUC2) and the constitutive Cauliflower Mosaic Virus 35S (pCaMV35S) promoter. Of all the transgenic atsuc2 plants, only pAtSUC2: OsSUT1 complemented the atsuc2 mutant phenotype in a comparable manner to wild type (WT), and consistent levels of soluble sugars and starch were recovered compared to those of WT. This suggests that OsSUT1 is a functional ortholog of the Arabidopsis AtSUC2 and functions as an apoplastic phloem loader. In addition, ossut1 mutants were produced via anther culture and their primary carbohydrate levels and growth phenotypes were indistinguishable from those of WT. This suggests that the rice phloem loader OsSUT1 function may not be essential for rice vegetative growth under normal conditions.
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Functional analysis of a cold-responsive rice WRKY gene, OsWRKY71
김치열,김성룡,정동훈,이상규,VO THI XUAN KIEU,Cong Danh Nguyen,Manu Kumar,박수현,전종성,김주곤 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.1
Publicly available microarray data and RNA gel blot analysis identified a rice WRKY transcription factor, OsWRKY71, that is highly upregulated in response to cold stress. Experiments with OsWRKY71 promoter: GFP transgenic rice confirmed its cold-inducible expression. Transient expression of OsWRKY71-GFP in maize mesophyll protoplasts indicated that it is localized predominantly in the nucleus and to a lesser extent in the cytosol. Transcriptional activation assays revealed that OsWRKY71 suppresses luciferase reporter activity in maize protoplasts, suggesting that it functions as a transcriptional repressor in rice. To characterize the function of OsWRKY71 in rice, we generated transgenic rice plants carrying CaMV35S promoter:OsWRKY71. Upon cold (4℃) treatment, two selected OsWRKY71 transgenic lines, OX12 and OX21, recovered much better with respect to survival rate, photosynthetic ability, fresh weight, and dry weight than the control lines. RT-PCR analysis of known cold-responsive genes found that expression of OsTGFR and WSI76 was increased in OsWRKY71 transgenic lines in response to cold stress. Our results suggest that OsWRKY71 has a positive function in cold tolerance by regulating downstream target genes.
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