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RISS 인기검색어
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- 저자 : Claudia Corvalán,Soon Il Kwon,Haerim Kim,Doyeon Kim,Jewook Woo,Sunghwa Choe (검색결과 3 건)
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Claudia Corvalán,Soon Il Kwon,Haerim Kim,Doyeon Kim,Jewook Woo,Sunghwa Choe 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Hormones play a crucial role in controlling physiological processes, and thus plants grow and develop in response to environmental cues through the interlocked actions of the hormones. Brassinosteroids (BRs) were found as growth-promoting steroid hormones. Rice, as a monocotyledonous model plants and the major staple crop, has been used to study BR action mechanisms. However, many components of BR pathways and the mechanisms of their molecular interactions have yet to be fully understood. Because the use of the BR biosynthetic inhibitor, Brassinazole (Brz), allowed us to identify important components of BR signaling such as the transcription factor BZR1, we decided to employ a similar strategy to identify novel signaling factors using propiconazole (Pcz), a new potent BR inhibitor. We screened a rice T-DNA mutant population which belongs to Dongjin variety and were developed by the Gene An’s group using pGA2715 T-DNA vector. Using Pcz treatments we searched for resistant plants, which were reflected on their lengths of roots and/or leaves. We isolated a total of 17 mutant lines, which are being analyzed phenotypically and at molecular level. So far, we have been able to found various lines presenting high or low yield compared to their wild type counterparts. We have found differences in panicle organization of these mutants. Our current experiments include the confirmation of Pcz resistance of these lines and molecular studies involving BR marker genes to understand the relation among yield and BR action in rice.
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Kim, Bokyung,Jeong, Yu Jeong,Corvalá,n, Claudia,Fujioka, Shozo,Cho, Seoae,Park, Taesung,Choe, Sunghwa BlackWell Publishing Ltd 2014 The Plant journal Vol.77 No.5
<P>Light is essential for plant survival; as such, plants flexibly adjust their growth and development to best harvest light energy. Brassinosteroids (BRs), plant growth-promoting steroid hormones, are essential for this plasticity of development. However, the precise mechanisms underlying BR-mediated growth under different light conditions remain largely unknown. Here, we show that darkness increases the activity of the BR-specific transcription factor, BZR1, by decreasing the phosphorylated (inactive) form of BZR1 in a proteasome-dependent manner. We observed that COP1, a dark-activated ubiquitin ligase, captures and degrades the inactive form of BZR1. In support of this, BZR1 is abundant in the <I>cop1-4</I> mutant. The removal of phosphorylated BZR1 in darkness increases the ratio of dephosphorylated to phosphorylated forms of BZR1, thus increasing the chance of active homodimers forming between dephosphorylated BZR1 proteins. Furthermore, a transcriptome analysis revealed the identity of genes that are likely to contribute to the differential growth of hypocotyls in light conditions. Transgenic misexpression of three genes under the <I>35S</I> promoter in light conditions resulted in elongated petioles and hypocotyls. Our results suggest that light conditions directly control BR signaling by modulating BZR1 stability, and consequently by establishing light-dependent patterns of hypocotyl growth in Arabidopsis.</P>
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DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
Woo, Je Wook,Kim, Jungeun,Kwon, Soon Il,Corvalá,n, Claudia,Cho, Seung Woo,Kim, Hyeran,Kim, Sang-Gyu,Kim, Sang-Tae,Choe, Sunghwa,Kim, Jin-Soo Nature Publishing Group, a division of Macmillan P 2015 Nature biotechnology Vol.33 No.11
Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation.
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