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- 저자 : Chunmei Zhong (검색결과 4 건)
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Research on Traffic Journey Intensity of Residents Based on Gravity Model
Ying Lin,Chunmei Zhong,Yong Liu 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.11
In order to accurately predict the traffic journey intensity of residents along the highway, according to the mechanisms of production of the traveling, analyze the influences of traffic journey intensity of residents from the three angles including residents' willingness to travel, destination’s attraction, traffic resistance, and predict the traveling intensity combined with the unconstrained gravity model. Then introduce the sensitivity of travel cost and analyze the impact of sensitivity of traveling cost on the traffic journey intensity. Finally, with an instance of the radiation areas of Zhaoma highway in Yunnan, calculate the calibration parameters in the unconstrained gravity model combined with the increase method and analyze the sensitivity of traveling cost within the scope of radiation areas. The results show that there is significant exponential relationship between the traffic journey intensity and the sensitivity, and it can provide some theoretical basis for the trip distribution; the gravity model is reliable and viable in some degree, and has a certain guiding significance for planning the road infrastructures.
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Meng Zheng,Zhiyuan Zhong,Chunmei Yang,Fenghua Meng,Rui Peng 한국고분자학회 2012 Macromolecular Research Vol.20 No.3
1.8 kDa branched polyethylenimine (PEI) was modified with pH-sensitive degradable acetal containing hydrophobe, 2,4,6-trimethoxybenzylidene-tris(hydroxymethyl)ethane (TMB-THME), to enhance its DNA condensation under extracellular conditions as well as to achieve active DNA release inside cells. PEI-(TMBTHME)n conjugates in the amount of 1.8 kDa were prepared with varying degrees of substitution (DS) from 3.0, 5.7 to 10.1. Notably, dynamic light scattering (DLS) measurements showed that all three 1.8 kDa PEI-(TMB-THME)n conjugates could effectively condense DNA into nano-sized particles (189-197 nm) at N/P ratios ranging from 20/1 to 80/1. The surface charges of PEI-(TMB-THME)n polyplexes depending on DS and N/P ratios varied from +22 to +28 mV,which were comparable to or slightly higher than the unmodified 1.8 kDa PEI counterparts (~+22 to +23 mV). Under a mildly acidic condition mimicking that of endosomes, interestingly, 1.8 kDa PEI-(TMB-THME)n polyplexes were quickly unpacked to release DNA because of the pH-induced acetal degradation that transforms hydrophobic modification into hydrophilic modification. MTT assays demonstrated that all PEI-(TMB-THME)n polyplexes displayed low cytotoxicity (>80%) to 293T, and HeLa cells at N/P ratios ranging from 20/1 to 60/1. The in vitro gene transfection studies showed that the transfection activity of 1.8 kDa PEI was significantly enhanced by modifications with TMBTHME,in which transfection efficiencies increased with increasing DS. For example, 1.8 kDa PEI-(TMBTHME)10.1 polyplexes displayed 250-fold and 80-fold higher transfection efficiencies than those of the unmodified 1.8 kDa PEI counterparts in 293T and HeLa cells, respectively, which were approximately 4-fold and 2-fold higher than that of 25 kDa PEI control. The superior transfection activity of 1.8 kDa PEI-(TMB-THME)10.1 polyplexes was also confirmed by confocal laser scanning microscopy (CLSM), which showed efficient delivery of DNA into the nuclei of 293T cells following 4 h transfection. Modification of low molecular weight PEI with pH-sensitive degradable hydrophobe has appeared to be highly promising in the development of “artificial viruses” for safe and efficient gene transfer.
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Study on Determination of Demand Intensity for Tourist Transit of Open Expressway Service Area
Yong Liu,Ying Lin,Runmin Zhang,Chunmei Zhong 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.4
In order to forecast the demand intensity for tourist transit of open expressway service area more accurately, according to the generation principle of tourism intensity, fully considering the influence of three forces including tourists travel demand thrust, tourism cities attraction and travel cost resistance on the strength of tourism, combining gravity model and giving a new meaning to the supply and demand parameters of the prediction model, this paper proposed a new model based on the three interaction forces to predict the demand intensity for tourist transit. In the face of lack of the status quo of tourism data, in order to verify the validity of the model, the method transforming the tourist receipts of tourist city into tourist traffic generation of a particular city was put forward. Finally, in the instance of Shuifu city of Yunnan province, this paper predicted the strength of tourists to travel in Shuifu. Numerical results showed that the model can be calibrated by the instance data and well explain the generation and distribution mechanism of traffic. This work can provide a certain basis for traffic service facilities planning.
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Junhui Wan,Junli Qin,Qinyue Cao,Ping Hu,Chunmei Zhong,Chunhua Tu 한국유전학회 2020 Genes & Genomics Vol.42 No.3
Background Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2) was induced in hypoxia and participated in cancer development. However, the role of PLOD2 in endometrial carcinoma remains unclear. Objective To explore the influences and regulation mechanism of PLOD2 in endometrial carcinoma under hypoxic condition. Methods The small interfering RNA (siRNA) targeting to PLOD2 and pcDNA3.1-PLPD2 were transfected to endometrial carcinoma cells to alter PLOD2 expression. Cell proliferation ability was determined by colony formation assay. Wound healing assay used to detect cell migration ability. Transwell invasion assay was used to detect cell invasion ability. Results PLOD2 and Hypoxia-inducible factor-1α (HIF-1α) were induced by hypoxia. Down-regulation of PLOD2 did not affect endometrial carcinoma cell proliferation ability, while inhibited cell migration, invasion under hypoxic condition. Besides, down-regulation of PLOD2 increased the levels of γ-catenin and E-cadherin and decreased levels of Fibronectin and Snail under hypoxic condition. Down-regulation of PLOD2 also inactivated Src and phosphoinositide 3-kinase (PI3K)/ protein kinase B (Akt) signaling under hypoxic condition. The promoting effects of PLOD2 overexpression on migration, invasion and epithelial-mesenchymal transition (EMT) of endometrial carcinoma cells were reversed by Akt inhibitor (MK2206) under hypoxic condition. Conclusion PLOD2 expression was increased in endometrial carcinoma cells under hypoxic condition. PLOD2 modulated migration, invasion, and EMT of endometrial carcinoma cells via PI3K/Akt signaling. PLOD2 may be a potential therapeutic target for endometrial carcinoma.
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