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Cha, Dong Seok,Hollis, Sarah E.,Datla, Udaya Sree,Lee, Sejin,Ryu, Jinsun,Jung, Hey Rim,Kim, Eunsuk,Kim, Kyuhyung,Lee, Myeongwoo,Li, Chris,Lee, Myon-Hee Elsevier 2012 Gene expression patterns Vol.12 No.5
<P><B>Highlights</B></P><P>► TOP-1β is broadly expressed in the nuclei of cells and concentrated in nucleoli. ► TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells. ► TOP-1 proteins are required for chromosomal segregation and germline development in <I>Caenorhabditis elegans</I>.</P> <P><B>Abstract</B></P><P>DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. <I>Caenorhabditis elegans</I> TOP-1 has two isoforms, TOP-1α and TOP-1β. TOP-1β is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that <I>top-1</I> transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1α in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that <I>C. elegans</I> TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms.</P>