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피부손상 치유시 진피층내 Fibronectin, Type I 및 Type III Collagen의 발현에 대한 면역전자현미경적 연구
엄기일,조임철,김잉곤,정호심,이선우,류재만 韓國非破壞檢査學會 1998 Archives of Plastic Surgery Vol.25 No.7
This study was focused on the localizations or expressions of fibronectin and interactions of fibronectin with type I and type Ⅲ collagen in the new forming tissues during wound healing in rat skin. Adult male rats(SPrague-Dawley strain), 150∼200 gm weight, were used for experimental animals. And the experimental animals were divided into 3 or 4 groups by passed days after incision of back skin. The specimens were obtained and immunohistologically stained for electron microscopy from each of groups and the specimens were observed with the electron microscope (Hitach-600 Model). The results obtained were as follows. 1. Strong positive reactions for type I collagen are seen in the new forming tissue at day 3 after incision of skin, and moderate reactions at day 5 and day 7 after incision of skin. 2. The weak reactions for type Ⅲ collagen are seen at day 3 and day 5 after incision in the new forming tissues of skin. And the type Ⅲ collagen reaction at day 7 are more increased than that of other groups. 3. Fibronectin expressions are seen moderately at day 5 and day 3 groups, and strong at day 5 group, but at day 7 group reactions are rapidly decreased. It is suggested that type I and Ⅲ collagens are gradually increased from the beginning of wound healing to the time of the new fibrous tissues formation. The great activities of the fibronection are seen within processes of the new tissue formation for wound healing events.