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Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2
Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.
Cho, Hyun-Jai,Lee, Choon-Soo,Kwon, Yoo-Wook,Paek, Jae Seung,Lee, Sun-Hee,Hur, Jin,Lee, Eun Ju,Roh, Tae-Young,Chu, In-Sun,Leem, Sun-Hee,Kim, Youngsoo,Kang, Hyun-Jae,Park, Young-Bae,Kim, Hyo-Soo American Society of Hematology 2010 Blood Vol.116 No.3
<B>Abstract</B><P>The concept of reprogramming of somatic cells has opened a new era in regenerative medicine. Transduction of defined factors has successfully achieved pluripotency. However, during the generation process of induced pluripotent stem (iPS) cells, genetic manipulation of certain factors may cause tumorigenicity, which limits further application. We report that that a single transfer of embryonic stem (ES) cell-derived proteins into primarily cultured adult mouse fibroblasts, rather than repeated transfer or prolonged exposure to materials, can achieve full reprogramming up to the pluripotent state without the forced expression of ectopic transgenes. During the process, gene expression and epigenetic status were converted from somatic to ES-equivalent status. We verified that protein-based reprogramming was neither by the contamination of protein donor ES cell nor by DNA/RNA from donor ES cell. Protein-iPS cells were biologically and functionally very similar to ES cells and differentiated into 3 germ layers in vitro. Furthermore, protein-iPS cells possessed in vivo differentiation (well-differentiated teratoma formation) and development (chimeric mice generation and a tetraploid blastocyst complementation) potentials. Our results provide an alternative and safe strategy for the reprogramming of somatic cells that can be used to facilitate pluripotent stem cell-based cell therapy.</P>
초고압 차단기용 코일건 타입 조작기의 기술적 타당성 검토
曺柱鉉(Chu-Hyun Cho),李弘植(Hong-Sik Lee),金錫煥(Seog-Whan Kim),金永培(Young-Bae Kim),李佑暎(Woo-Young Lee),金烘?(Hong-Kyu Kim) 대한전기학회 2006 전기학회논문지C Vol.55 No.7
A novel coil gun type actuator has been proposed and tested for its possibility as an actuator for a high voltage circuit breaker by experimental method. The 20kJ capacitor bank with the electrolytic condensers was charged up to 500V and discharged to a couple of 100 turn coils connected with parallel through a thyristor switch. The repulsive force between coil and the actuator made by copper conductor of 20㎏ reached up to 23,000 N, and over 800J of energy was transferred to the kinetic energy of the conductor. The experimental results showed that the coil gun type actuator has a good possibility for the high voltage circuit breaker.
曺柱鉉(Chu-Hyun Cho),金秉杰(Byung-Geol Kim),朴相河(Sang-Ha Park),姜忠日(Chung-Il Kang),李弘植(Hong-Sik Lee),林根熙(Geun-Hie im) 대한전기학회 2006 전기학회논문지C Vol.55 No.9
The technology of wire explosion have been used to product nanopowders. A new concept was proposed to produce metallic nanosized powders, which is wire explosion in liquid media. We have exploded the Ag or Cu wires of diameter of 0.3㎜, 40㎜ long, in the de-ionized water or acetone, respectively. Electrical energy of 1.1kJ was stored in 10㎌ capacitor and released to the wires through a triggered spark gap switch. The process was observed by high-speed camera. Those images showed that the powders were generated by vapor condensation in the shell formed by shock wave in the water. The particles were directly dispersed into the water with collapse of the shell. The sizes of Ag and Cu nanopowders were evaluated to 35㎚ and 17㎚, respectively.