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Study on Cellar Behaviors on Different Nanostructures by Nanoporous Alumina Template
Chiung-Fang Huang,Yung-Kang Shen,Hsin-Chung Cheng,Yi Lin,Chih-Wei Wu 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.
Nanoporous anodic aluminum oxide (AAO) templates are fabricated using an nodization method. The mean diameters of nanoporousanodic aluminum oxide templates are 100 nm and 200 nm by various processing parameters of the anodization method. A moldedplastic thin film nanostructure is fabricated by nanoimprinting using the AAO template as a mold insert. The surface properties ofthe molded plastic thin film are discussed using various nanoimprinting process parameters. Contact angles of the molded plastic thinfilm with the nanostructure exceed those without the nanostructure. The molded plastic thin films with a nanostructure and ahydrophobic surface are formed, and their contact angles exceed 90o. This study observes the behavior of osteoblast-like cells (MG63)cultured on nanostructure thin films, i.e., AAO, polylactic acid (PLA) and polycarbonate (PC). Cell growth behavior indicates thatthe AAO template with a 200 nm nanostructure is best. This study shows that cell adhesion and spreading are influenced by surfacetopography in the nanometer feature.