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        Nicotiana 속에 (屬) 있어서 Tetrazolium Oxidase Isozyme 의 전기영동적 (電氣泳動的) 변이에 관한 연구

        한창열(Chang Yawl Harn),손청열(Cheong Yeol Sohn),김영진(Yung Jin Kim) 한국식물학회 1975 Journal of Plant Biology Vol.18 No.4

        Four kinds of Nicotiana species and five varieties belonging to N. tabacum were used as materials for electrophoretic analysis of the tetrazolium oxidase isozyme to examine the taxonomic affinity among them based on the biochemical property. All the five varieties of N. tabacum showed same isosyme bands despite the fact that these varieties had notably varied characteristics including morphological traits. The band patterns were more or less different among the four species. Although N. rustica and N. tabacum were of the same genome group of AABB, their isozyme bands showed considerable difference. N. sylvestris, genome A donor of Nicotiana species, was found to be markedly different from N. tabacum in band pattern, including the absence of system 2 in N. sylvestris.

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        人爲突然變異 利用에 依한 小麥品種改良 Ⅰ. 小麥에 대한 γ 線과 EMS의 處理効果

        Kun Hyuk Im(任建爀),Shin Han Kwon(權臣漢),Cheong Yeol Sohn(孫淸烈),Jong Sun Eun(殷鍾旋) 한국육종학회 1969 한국육종학회지 Vol.1 No.1

        As basic research to select useful mutants, this experiment was conducted to investigate biological response in M₁seedlings of wheat varieties, Yongkwang and Jaekwang, treated with gamma ray and EMS. On dormant seeds, gamma ray treatment was performed at the dosage of 5, 10, 15, 20, 30, 40 and 50 kr, and EMS at the concentration of 0.05, 0.075, 0.10, 0.15, 0.20, 0.25 and 0.30 mole. No effect in germination rate was found in variety Yongkwang by gamma ray irradiation, while significant effect was observed in Jaekwang, although it was not in proportion to the dosage. In emergence rate, significant decrease was shown in both varieties with the gamma ray treatment. As the EMS concentration increased, significant reduction in germination and emergence rate was observed in both varieties. Germination speed was not influenced with the gamma ray treatment, whereas it was slowed with the increase in EMS concentration. In both varieties, seedling survival rate 45 days after sowing was not affected by either gamma ray or EMS treatment. The growth of young roots, seedlings and young leaves was gradually inhibited with the increase of gamma ray dose and EMS concentration. On the basis of the above results, 50 per cent growth reduction dosage or concentration was determined in this study, and it was found that radiosensitivities of the wheat was dependent on varieties and mutagens. 本 實驗은 小麥品種 永光과 再光에 gamma線과 EMS를 處理하여 有用變異體를 選拔하는 過程 中에 얻은 M₁幼植物體의 生物學的 反應을 調査한 것이다. (1) 發芽率에 對한 gamma線 處理效果는 永光에서는 없었으나 再光은 線量에 比例하지는 않으나 有意性이 있었고, 成苗率은 兩 品種 모두 有意한 減少를 보였다. 그리고 EMS處理效果는 發芽率과 成苗率이 兩 品種에서 濃度增加에 따라 모두 有意性있게 減少하였다. (2) 幼苗 生存率에 있어서는 gamma線과 EMS處理效果가 없었다. (3) 幼根, 幼苗 그리고 本葉의 生長은 gamma線과 EMS處理에 對한 障害가 處理量 增加에 따라 直線的으로 增加하였다. (4) 品種에 따라 mutagen에 對한 生物學的 反應이 相異할 뿐 아니라 同一品種 內에서도 mutagen 種類에 따라서 差異를 보이고 있다.

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