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RISS 인기검색어
- 검색키워드
- 저자 : Char Bharat (검색결과 3 건)
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Khanale Vaishali,Bhattacharya Anjanabha,Satpute Rajendra,Char Bharat 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4
Key message Transcription factors (TFs) are the key regulators in plant development and stress adaptation. Under- standing interactions of TFs in cotton crop is important for enhancing stress tolerance and yield enhancement. Among plant TFs, bZIPs plays a major role in seed germination, flower development, biotic and abiotic stress response. Most of the bZIP proteins from cotton remains uncharacterized and can be utilized for crop improvement. Bioinformatics analysis of bZIPs of cultivated cotton species G. hirsutum along with two sub-genome species G. arboreum and G. raimondii at one platform will certainly help the researchers in the selection of specific cotton bZIP genes according to the close alignment with Arabidopsis orthologs or sub-genome homolog for functional characterization. Abstract Cotton is an important commodity in the world economy. Transcription factors (TFs) are the key regulators in plant development and stress adaptation. Understanding interactions of TFs in cotton crop is important for enhancing stress tolerance and yield enhancement. Among plant TFs, bZIPs plays a major role in seed germination, flower development, biotic and abiotic stress response. Most of the bZIP proteins from cotton remains uncharacterized and can be utilized for crop improvement. In this study, we have carried out genome-wide identification and bioinformatics characterization of basic leu- cine zipper domain proteins (bZIPs) from cultivated cotton species Gossypium hirsutum along with two sub-genome species of allotetraploid cotton, Gossypium arboreum and Gossypium raimondii. A total of 228 bZIP genes of G. hirsutum, 91 bZIP genes of G. arboreum and 86 bZIP genes of G. raimondii were identified from CottonGen database. Cotton bZIP genes were annotated in standard pattern according to their match with Arabidopsis bZIPs. Multiple genes with similar bZIP designa- tions were observed in cotton, linked to the gene duplication. Cotton bZIPs are distributed across all 13 chromosomes with varied density. Phylogenetic characterization of all three cotton species bZIPs classified them into 12 subfamilies, namely A, B, C, D, E, F, G, H, I, J, K and S and further into eight subgroups according to their predicted functional similarities, viz., A1, A2, A3, C1, C2, S1, S2 and S3. Subfamilies A and S are having maximum number of bZIP genes, subfamilies B, H, J and K are single-member families. Cotton bZIP protein functions were predicted from identified motifs and orthologs from varied species. Basic region leucine zipper (BRLZ) domain analysis of G. raimondii bZIPs revealed the presence of conserved basic region motif N-X7-R/K in almost all subfamily members, variants are GrbZIP62 with N-X7-I motif and GrbZIP76 with K-X7-R motif. Leucine heptad repeats motif, are also present in variant numbers from two to nine with leucine or other hydrophobic amino acid at designated position among 12 subfamily members. STRING protein interaction network analysis of G. raimondii bZIPs observed strong interaction between A and D, B and K, and C and S subfamily members.
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Genome editing interventions to combat rice blast disease
Khanale Vaishali,Bhattacharya Anjanabha,Prashar Mohinder,Char Bharat 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.1
Rice is the major food crop of the world. Production of rice is threatened by a number of biotic and abiotic stresses and these could result in a significant yield reduction, sometimes up to 60–100%. In addressing losses to emerging diseases, development of resistance to blast, sheath blight, and false smut is the need of the hour. The traditional approach of using resistance R genes for building sustainable resistance is not viable anymore due to continuously evolving strain of pathogens, thus causing intransigence erosion. The contemporary intervention strategy of identifying and precisely mutating susceptibility genes opens up new vistas for exploiting susceptible genes. Approaches like mutation breeding and transgenic held good promise to further yield increases in rice and provide protection against disease induced yield losses. However, all these approaches either proved inadequate over time or faced regulatory hurdles and were successful to a limited point. Recently, a new genome editing (GE) approach has offered a promising alternative to develop unique alleles in a shorter time frame without linkage drag. Thus, the present review highlights various R, non-R (S) gene choices amenable for genome editing, compared with available conventional breeding approaches, like wide-hybridisation using wild relatives, including new genetic resources. Hence, a range of breeding resources can be employed by crop breeders to assemble disease resistance against emerging diseases of rice. Many successful examples on disease resistance are collated from recently published work. It is hoped that creating new allelic series of variants would be a practical and sustainable solution to address the challenges posed to food security.
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Molecular analysis of mitochondrial cytochrome oxidase I gene of Aedes aegypti L. mosquitoes
Ratnapal Gandhi,Kamlesh K. Yadav,Prabhakargouda B. Patil,Pankaj Bihani,Bharat Char,Shaibal K. Dasgupta,Usha B. Zehr,Shirish R. Barwale 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1
Aedes aegypti is the most important arboviral vector worldwide. Recent studies reported that genetic variations and gene flow among same mosquito species is responsible for different disease transmission rate. Hence, to understand the relationship between genetic diversity and disease transmission potential, study on genetic variations among mosquito populations is essential. The aim of present study was to investigate the genetic variations of Ae. aegypti targeting COI gene from nine villages of Jalna District, Maharashtra and three laboratory strains originated from Aurangabad, Delhi and transgenic OX513A strain imported from OXITEC, UK. OX513A strain consists of a self-limiting dominant lethal gene construct intended for its use in suppression of Ae. aegypti population by sustained male adult releases in the environment. Mosquito eggs from field and laboratory strains were reared to adults and identified on the basis of morphological characteristics followed by COI gene sequence. Result of MSA and haplotype analysis revealed low genetic variations among field samples and Aurangabad strain, belonged to two haplotypes (H1 and H2) except Ramkheda village represented by separate haplotype H3. Other laboratory DEL strain and transgenic OX513A have great genetic variability to all isolates and have a separate haplotypes H4 and H5. Similar results were observed in phylogenetic analysis. Our observation of phylogenies revealed close relationship among the DEL and transgenic strain OX513A with few Indian and worldwide isolates. The information on genetic variability of mosquito population could help to understand and design the strategies for risk mitigation and effective implementation of new vector control tools like genetically modified mosquitoes.
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