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Biochemical Studies of an Endoglucanase from Marine Rotifer, Brachionus plicatilis
Chun Chang Zoon,Park Heum Gi,Hur Sung Bum,Kim Young Tae The Korean Society of Fisheries and Aquatic Scienc 1996 韓國養殖學會誌 Vol.9 No.4
Cellulase was purified from marine rotifer, Brachionus plicatilis, to homogeneity by using chromatographic methods. Purified enzyme is an endo-${\beta}$-1,4 glucanase and shows a strong hydrolytic activity against carboxymethyl (CM) -cellulose. The physicochemical parameters of enzyme activity were determined. The molecular weight of the purified protein was approximately 62 kDa as determined by SDS-polyacrylamide gel electrophoresis. The enzymatic capability to digest cellulose of Chlorella cell wall was compared with that of other well known cellulases from Thermomonospora fusca. Experiments involving Chlorella digestion indicated that CM-cellulase from marine rotifer, Brachionus plicatilis, could digest Chlorella very efficiently while cellulase purified from Thermomonospora fusca did not. From the result here, we propose that the cellulolytic system from marine rotifer is responsible for the hydrolysis of cellulosic wall of Chlorella, probing that rotifer digests Chlorella as a major live food.
Marine Biotechnology: from Molecules to Aquaculture and Biomedicine
Chen, Thomas T.,Chun, Chang Zoon,Chiou, Peter,Chen, Maria J. The Korean Society for Marine Biotechnology 2006 한국해양바이오학회지 Vol.1 No.1
Marine biotechnology encompasses biotechnology in areas such as marine microbiology, biomedical important marine natural products, organisms in extreme environments, and aquaculture. Marine biotechnology, today, poised to flourish more than ever from the confluences that are occurring in fundamental research in modern biology and other areas of science. Using research results from our laboratory and those from others, we will review the current advances of marine biotechnology in this lecture.