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Ajeesh Chandrasekharan,이경원,김소담,이혜선,안선영,양승윤 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Three dimensional (3D)-bioprinting offers an innovative stratergy to fabricate functional tissues and organs with high complexicity and precision. Within this framework, digital light processing (DLP) technique using photocrosslinkable bioinks consisting of photosensitive biopolymers and photoinitiators (PIs) allows to prepare 3D complex hydrogels with tissue-specific properties. However, poor biocompatibility and low photoreactivity of the available bioinks limit their use in the fabrication of cell-laden 3D tissue constructs. Here, we report a fast-curable low-energy bioprinting system using a photosensitive hyaluronic acid (HA) and type I PIs based on benzoyl phosphinate for visible light-induced 3D bioprinting. After examining the water solubility, molar extinction coefficients, photocrosslinking efficiency and biocompatibility of the PIs, curved corneal structure was fabricated and the viability of corneal stroma cells in the 3D-printed constructs were evaluated. Overall, the HA-based photoreactive bioinks exhibited superior properties to function as a fast-curing visible light-induced 3D printing for use in tissue engineering applications.
Selvakumar T.,Nataraju B.,Chandrasekharan K.,Sharma S. D.,Balavenkatasubbaiah M.,Sudhakara Rao P.,Thiagarajan V.,Dandin S. B. Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.11 No.1
While observing silkworm larval samples received from field, microsporidian spores formed within the haemocytes of silkworm haemolymph were observed. The spores of microsporidian sp. were purified and characterized for morphological characters viz., size, shape as well as serological affinity with different Nosema spp. (M$_{11}$ and M$_{12}$). The infectivity of the isolated spores to silkworm was also studied. The microsporidian sp. was found to be highly pathogenic to silkworm, B. mori. The isolated microsporidian sp. was designated as NIK-5hm, which formed ovocylindrical spore in the haemocytes of silkworm and differed in spore size (length, 4.55 $\mu$m & width, 2.10 $\mu$m) and shape from Nosema bombycis (NIK-ls), NIK-2r (Nosema sp. Mysore [3.6 & 2.8 $\mu$m]), NIK-3h (Nosema sp. M$_{11}$ [3.8 & 1.8 $\mu$m]), NIK-4m (Nosema sp. M$_{12}$ [5.0 & 2.1 $\mu$m]) and Lb$_{ms}$ (Nosema sp. in Lamerine breed of silkworm [4.36 & 2.14]). In immonological test (Latex agglutination test), the isolated microsporidian spores did not react with antibody sensitized latex particles of N. bombycis, M$_{11}$, M$_{12}$ and Lb$_{ms}$ and thus are different type of microsporidian sp., parasitic to silkworm, Bombyx mori L.