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Analysis of regeneration protocols for micropropagation of Pterocarpus santalinus
Chakraborty Tanushree,Chaitanya K. Viswanatha,Akhtar Nasim 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.1
A micropropagation is a powerful tool in the era of the biotechnology revolution. It has a broad range of potentiality as compared to conventional vegetative propagation attracting researchers, industrialists, governmental and nongovernmental organizations at the national and international level. The potential methods of organogenesis and somatic embryogenesis, primarily through callogenesis, allow the production of genotypically identical and pharmacologically conserved disease- free healthy stocks in shorter times. Pterocarpus santalinus, the pride of Andhra Pradesh, has become endangered due to medicinal and commercial overexploitation. The micropropagation of P. santalinus poses many cultural challenges due to limited regeneration potential through callogenesis, organogenesis, and somatic embryogenesis. The lack of proper explant treatment and the effect of plant growth regulators limit the application of published protocols to reproduce the results. The challenge, such as heavy contamination of mature explants with endophytic fungi, forced us to explore the potential of immature tissues for regeneration through induction of somatic embryogenesis. We observed that immature tissues (zygotic embryo, petal, ovary, and anther) are better responsive than mature tissues with the scantiest contamination and phenolic release. The present study analyzed, evaluated, and interpreted the different parameters applied in the micropropagation of P. santalinus. The aim is to solve the discrepancies of existing protocols to present complete insight for future needs in the successful regeneration of the species. The review also compared various treatments to overcome dormancy and promote germination. It also discussed the possibilities of induction of somatic embryogenesis for future research.
Mutagens in commercial food processing and its microbial transformation
Narendra Kishore Merugu,Saikumar Manapuram,Tanushree Chakraborty,Sita Kumari Karanam,Sarat Babu Imandi 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.5
Mutagens are chemical molecules that have the ability to damage DNA. Mutagens can enter into our body upon consumption of improperly cooked or processed food products such as high temperature or prolonged cooking duration. Mutagens are found in the food products can be classified into N-nitroso derivatives, polycyclic aromatic hydrocarbons, and heterocyclic aromatic amines. Food products with high fat and protein content are more prone to mutagenic formation. Microorganisms were found to be a potent weapon in the fight against various mutagens through biotransformation. Therefore, searching for the microorganisms which have the ability to transform mutagens and the development of techniques for the identification as well as detection of mutagens in food products is much needed. In the future, methods for the identification and detection of these mutagens as well as the identification of new and more potent microorganisms which can transform mutagens into non-mutagens are much needed.