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Luis Sa´enz,Carlos Puch-Hau,Carlos Oropeza,Manuel Go´ngora-Paredes,Iva´n Co´rdova,Jose´ Tun-Sua´rez 한국유전학회 2016 Genes & Genomics Vol.38 No.9
The nucleotide binding site and leucine rich repeat (NBS–LRR) class of R genes is the most comprehensively studied in terms of sequence evolution; however, in coconut palm and, more generally, in the family of Arecaceae, our understanding of the evolution of these genes is rather limited. In this study, disease resistance gene candidates (RGCs) of the nucleotide binding site (NBS) type of coconut palm were used to investigate evolutionary relationships in Arecaceae, Poaceae and Brassicaceae species. The results indicate a speciesspecific evolution of RGCs in coconut palm. However, strikingly similar RGCs between species of Arecales indicate a high conservation of specific RGCs of this family, suggesting a monophyletic origin of three genera. The phylogenetic relationship between RGCs of Arecales and Brassicales suggests that these sequences possibly emerged before being divided between monocots and dicots. Finally the comparative analysis of the expression of four RGCs in healthy coconut palm and those affected with lethal yellowing disease revealed differences in their expression profiles. This study provides new insights for future efforts towards the improvement of disease resistance in coconut palm and other species of Arecaceae.
Germán Nic‑Matos,María Narváez,Santy Peraza‑Echeverría,Luis Sáenz,Carlos Oropeza 한국유전학회 2017 Genes & Genomics Vol.39 No.9
Nonexpressor of pathogenesis-related gene 1 (NPR1) codes for a transcription cofactor involved in the activation of systemic acquired resistance, a salicylic acid (SA)-dependent defense response. This work reports the cloning and characterization of two new genes, CnNPR1 and CnNPR3 from coconut, homologous to AtNPR1 of Arabidopsis thaliana. The cDNA deduced amino acid sequence contains the protein–protein interaction domains the BTB/POZ and ANKYRIN repeat domains, and a nuclear localization site (NLS). Phylogenetic analysis grouped CnNPR1 in a clade with AtNPR1 and CnNPR3 in a clade with AtNPR3, both reported genes of A. thaliana. Exogenous application of SA to coconut plantlets induced changes in the expression of CnNPR1 and CnNPR3 in leaf, stem and root tissues, providing evidence of their possible role in the signaling cascade leading to SAR in coconut palm. This is the first report on the cloning of putative key genes in the SAR-type defense mechanism in coconut palm.