Role of Leu188 in the Fatty Acid Hydroxylase Activity of CYP102A1 from Bacillus megaterium

Jang, H.H.,Shin, S.M.,Ma, S.H.,Lee, G.Y.,Joung, Y.H.,Yun, C.H. Elsevier 2016 Journal of molecular catalysis Enzymatic Vol.133 No.-

<P>P450 BM3 (CYP102A1) from Bacillus megaterium catalyzes the subterminal hydroxylation of fatty acids with 12-22 carbons at the omega-1, omega-2 and omega-3 positions. Several amino acids located at the substrate channel and active sites are known to be important for the catalytic activity of CYP102A1. The L188 residue at the C-terminus of alpha-helix F undergoes a large shift upon substrate binding and has frequently been found in different combinations of multiple mutations showing enhanced and altered activities. In this study, we examined the role of the L188 residue by comparing the catalytic activities of wild-type CYP102A1 and 19 mutants of L188. The mutants were made with site-directed mutagenesis and functionally expressed in Escherichia coll. The enzymatic properties of the mutants for a set of fatty acids (C-10-C-16) were compared to the properties of the wild-type. L188Q and L188 P mutants showed especially strong increases in hydroxylase activity toward C-10-C-13 fatty acids, although they did not have activity changes for C-14-C-16 fatty acids. Although most mutants showed very similar overall hydroxylation rates for myristic acid, 14 mutants showed apparent changes in the regioselectivity of hydroxylation with a preference for the omega-3 position over the omega-1 position. A possible role for the L188 residue has been discussed in the context of the structure and function of CYP102A1. (C) 2016 Elsevier B.V. All rights reserved.</P>

Gender differences in coronary plaque components in patients with acute coronary syndrome: Virtual histology-intravascular ultrasound analysis

Hong, Y.J.,Jeong, M.H.,Choi, Y.H.,Ma, E.H.,Cho, S.H.,Ko, J.S.,Lee, M.G.,Park, K.H.,Sim, D.S.,Yoon, N.S.,Youn, H.J.,Kim, K.H.,Park, H.W.,Kim, J.H.,Ahn, Y.,Cho, J.G.,Park, J.C.,Kang, J.C. Japanese College of Cardiology 2010 Journal of cardiology Vol.56 No.2

Background: The aim of this study was to evaluate the gender differences in plaque components in acute coronary syndrome (ACS) patients. Methods: We used virtual histology-intravascular ultrasound to evaluate the plaque components in culprit lesions in 362 ACS patients (254 men, 108 women). Results: Women were more likely to be diabetic (34% vs 23%, p=0.030), had greater percentage necrotic core (%NC) volume (19.0+/-12.7% vs 16.8+/-11.9%, p=0.040), and had trends toward higher high-sensitivity C-reactive protein (hs-CRP) (0.85+/-1.28mg/dl vs 0.53+/-0.48mg/dl, p=0.063), and higher incidence of thin-cap fibroatheroma (TCFA) (62% vs 52%, p=0.078) compared with men. %NC volume was significantly greater in diabetic patients compared with nondiabetic patients (20.4+/-10.2% vs 16.0+/-8.9%, p<0.001) and was significantly greater in patients with elevated hs-CRP (>=0.2mg/dl) compared with those with normal hs-CRP (<0.2mg/dl) (18.8+/-8.9% vs 16.6+/-9.7%, p=0.021). However, there were no differences in plaque components between diabetic women and men, and between women and men with elevated hs-CRP levels. Diabetes [odds ratio (OR): 2.44, 95% confidence interval (CI): 1.35-3.82, p=0.003] and hs-CRP (OR: 1.54, 95% CI: 1.08-2.65, p=0.032), but not female gender, were the independent predictors of TCFA. Conclusions: Although it seems likely that female ACS patients have more vulnerable plaque components compared with male ACS patients, these findings may result not from true gender differences in plaque components but higher prevalence of diabetes and hs-CRP elevation in women.

Age-related differences in virtual histology-intravascular ultrasound findings in patients with coronary artery disease

Hong, Y.J.,Jeong, M.H.,Choi, Y.H.,Ma, E.H.,Ko, J.S.,Lee, M.G.,Park, K.H.,Sim, D.S.,Yoon, N.S.,Youn, H.J.,Kim, K.H.,Park, H.W.,Kim, J.H.,Ahn, Y.,Cho, J.G.,Park, J.C.,Kang, J.C. Japanese College of Cardiology 2010 Journal of cardiology Vol.55 No.2

Background: We assessed the age-related differences in pre-intervention virtual histology-intravascular ultrasound (VH-IVUS) findings at target lesion sites in patients with coronary artery disease. Methods: A total of 553 patients who underwent pre-intervention VH-IVUS imaging were grouped according to age: non-elderly (@?70 years, n=429) and elderly (>70 years, n=124); 191 had stable angina and 362 acute coronary syndrome. VH-IVUS classified the tissue into: fibrotic, fibro-fatty, dense calcium (DC), and necrotic core (NC). Results: Overall, the absolute and percent volumes of DC (11.0+/-11.0mm<SUP>3</SUP> vs. 9.7+/-11.9mm<SUP>3</SUP>, P=0.033; 11.7+/-8.1% vs. 9.8+/-7.2%, P=0.014, respectively) and NC (18.5+/-17.6mm<SUP>3</SUP> vs. 16.6+/-18.9mm<SUP>3</SUP>, P=0.020; 18.8+/-8.8% vs. 16.5+/-9.3%, P=0.026, respectively) were significantly greater in the elderly than in the non-elderly. In stable angina patients, the absolute and percent volumes of DC (10.4+/-9.9mm<SUP>3</SUP> vs. 7.2+/-7.6mm<SUP>3</SUP>, P=0.022; 13.4+/-10.0% vs. 9.2+/-6.5%, P=0.011, respectively) and NC (14.8+/-11.2mm<SUP>3</SUP> vs. 12.0+/-11.9mm<SUP>3</SUP>, P=0.035; 19.6+/-8.8% vs. 15.5+/-8.4%, P=0.006, respectively) were significantly greater in the elderly. However, in acute coronary syndrome patients, there were no significant differences in absolute and percent volumes of DC (11.4+/-11.6mm<SUP>3</SUP> vs. 10.9+/-13.4mm<SUP>3</SUP>, P=0.8; 10.7+/-6.5% vs. 10.1+/-7.5%, P=0.5, respectively) and NC (24.1+/-20.3mm<SUP>3</SUP> vs. 23.9+/-21.2mm<SUP>3</SUP>, P=0.9; 22.0+/-8.8% vs. 21.3+/-9.6%, P=0.6, respectively) between the elderly and non-elderly groups. Myocardial infarction (OR: 2.56, 95% CI: 1.45-4.12, P=0.003), diabetes mellitus (OR: 2.23, 95% CI: 1.30-3.53, P=0.009), and high-sensitivity C-reactive protein (OR: 1.44, 95% CI: 1.06-2.45, P=0.042), but not age, were independent predictors of percent NC volume >20% in lesion site. Conclusions: Myocardial infarction, diabetes mellitus, and high-sensitivity C-reactive protein, but not age, were associated with NC-rich lesions. Clinical presentation, risk factors, and inflammatory status, but not age, are important factors for plaque components.

Chemical synthesis of Nd2Fe14B hard phase magnetic nanoparticles with an enhanced coercivity value: effect of CaH2 amount on the magnetic properties

Jeong, J.,Ma, H.,Kim, D.,Kim, C.,Kim, I.,Ahn, J.,Kim, D.,Kang, Y. Royal Society of Chemistry 2016 NEW JOURNAL OF CHEMISTRY Vol.40 No.12

<P>Nd2Fe14B hard phase magnetic nanoparticles were successfully synthesized using a chemical synthesis route followed by a reduction and diffusion process without consuming a large amount of energy. Nd(acac)(3)center dot xH(2)O, Fe(acac)(3) and (C2H5)(3)NBH3 were used as precursors and CaH2 was used as a reducing agent for the reduction and diffusion process. The crystal structure and composition of the resultant Nd-Fe-B nanoparticles were determined using X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX) measurements. The overall morphology and magnetic properties of the final products were investigated using transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM) at 300 K. The effect of the relative quantity of CaH2 on the magnetic properties of the final products was studied in detail with different weight ratios of CaH2 to the preliminary reduced metal oxide powders, using high resolution TEM (HRTEM), XRD and VSM measurements. The different mechanisms of the reduction and diffusion process with different amounts of CaH2 were comparatively studied. An insufficient amount of CaH2 resulted in the presence of residual unreduced metal oxide powders, decreasing the magnetic coercivity. On the other hand, an excessive amount of CaH2 caused H-2 gas to be evolved during the washing process with water, resulting in hydrogenation of the Nd2Fe14B to produce Nd2Fe14BHx. The relative amount of CaH2 had a critical effect on the magnetic properties of the prepared Nd2Fe14B-nanoparticles.</P>

Deconvolution of Four Transmission-Line-Model Impedances in Ni-YSZ/YSZ/LSM Solid Oxide Cells and Mechanistic Insights

Shin, E.C.,Ma, J.,Ahn, P.A.,Seo, H.H.,Nguyen, D.T.,Lee, J.S. Pergamon Press 2016 ELECTROCHIMICA ACTA Vol.188 No.-

Four impedance components with the two respectively from the cathode and anode have been successfully deconvoluted for the temperature range between 800<SUP>o</SUP>C and 600<SUP>o</SUP>C of cermet-supported (H<SUB>2</SUB>,H<SUB>2</SUB>O)Ni-YSZ/YSZ/LSM-YSZ(O<SUB>2</SUB>,N<SUB>2</SUB>) solid oxide fuel cells with high quality 5μm thin YSZ electrolyte layers with two different pore former contents of 10 and 20wt%. A Bisquert transmission line model in an ideal three-parameter equation was successfully applied for the electrochemical reactions for both Ni-YSZ and LSM-YSZ electrodes. Non-faradaic chemical reaction mechanism provided the hydrogen dissociation surface reaction energy of 0.58eV with adsorption capacitance activated with 0.4~0.5eV in Ni-YSZ electrodes directly by impedance spectroscopy. Much higher adsorption capacitance of LSM-YSZ electrode explains the lower frequency response and may explain strongly activated kinetic parameters. The surface diffusivity is effectively enhanced by the YSZ network in the composite electrodes. Gas concentration impedance of Ni-YSZ cermets described by ideal Gerischer model is largely responsible for the performance under light current load condition at high temperature and the higher porosity led to lower resistance but to higher capacitance. Gas concentration polarization may also appear diffusion-reaction co-limited and thinner LSM-YSZ electrode layer exhibits the higher capacitance than the thicker cermet support by two orders of magnitude.

遊離頭皮 被瓣術을 利用한 前方前頭部의 毛線再建 治驗例

馬興洙,金鎭喆,林豊 大韓成形外科學會 1984 Archives of Plastic Surgery Vol.11 No.1

Facial burn often brought about both loss of hair bearing scalp and obliteration of anterior frontal hairline which resulted esthetic deformity to the patients with many social and psychological problems. For many decades plastic surgeons have used a variety of method (such as single hair transplantation, punch or strip graft, and local flaps) to solve these problems. But the results are commonly unsatisfactory due to the poor vascularity, which leads to partial loss of the flap, unnatural hair direction and sparse desity. However, since the introduction of microsurgery, Harii applied a new method of microvascular free scalp transfer and reported its satisfactory results in 1974. The authors performed this microvascular free scalp flap transfer to a 8 years old boy who had lost extensive scalp hair to deep burn. Therefore the authors made this case report with the favorable result.

長掌腱(palmaris Longus Tendon)을 이용한 先天性 眼瞼下垂症의 治驗例

鄭求忠,馬興洙,李鐘建,李在雄 大韓成形外科學會 1986 Archives of Plastic Surgery Vol.13 No.3

The result of the surgical correction of the congenital blepharoptosis is determined by the nature of ptosis, operative method, operator's technique and post operative care. Among the factors levator function is most important, therefore, preoperative assessment of the levator function and surrounding structures must be carefully performed for the best operative result. Frontalis suspensions using autogenous fascia have been widely used in the cases whose levator funtions are less than 2mm. But the method may develop some postperative problems; recurrence due to absorption or loosening of fascia lata, complexity of the operative procedures, herniation of the muscles in lateral thigh and hematoma. Because of these problems, many considerations were prompted to use the autogenous tendon. Authors report 3 cases of congenital blepharoptosis who have been satisfactorily corrected by frontalis suspension method using palmaris longus tendon.

AZO(ZnO:Al)투명도전막의 전기적 및 광학적 특성

이형기,마대영,박기철 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

Highly conductive and transparent films have been fabricated by RF magnetron sputtering from the ZnO targets mixed with Al_(2)O_(3) ranging from Owt.% to 5wt.%. And electrical and optical properties of them have been investigated as functions of deposition conditions. Optimized AZO films 1500A thick had optical transmittance of 90% at 550nm and Hall resistivity of 4.7x10^(-4)Ω.cm.

NMR Solution Structure of HP0827 (O25501_HELPY) from Helicobacter pylori: Model of the Possible RNA-binding Site

Jang, S.-B.,Ma, C.,Lee, J.-Y.,Kim, J.-H.,Park, S. J.,Kwon, A.-R.,Lee, B.-J. Oxford University Press 2009 The Journal of biochemistry Vol.146 No.5

<P>The HP0827 protein is an 82-residue protein identified as a putative ss-DNA-binding protein 12RNP2 Precursor from Helicobacter pylori. Here, we have determined 3D structure of HP0827 using Nuclear Magnetic Resonance. It has a ferredoxin-like fold, beta1-alpha1-beta2-beta3-alpha2-beta4 (alpha; alpha-helix and beta; beta-sheet) and ribonucleoprotein (RNP) motifs which are thought to be important in RNA binding. By using structural homologues search and analyzing electrostatic potential of surface, we could compared HP0827 with other RNA-binding proteins (sex-lethal, T-cell restricted intracellular antigen-1, U1A) to predict RNA-binding sites of HP0827. We could predict that beta sheets of HP0827, especially beta1 and beta3, are primary region for RNA binding. Consequently, similar to other RNA-binding proteins, RNP motifs (Y5, F45, F47), positively charged and hydrophobic regions (K32, R37, K40, K41, K43, R70, R73) are proposed as a putative RNA-binding sites. In addition, differences in amino acids composition of RNP motifs, N, C-terminal residues, loop-region fold and the orientation of alpha1-helix with other RNA recognition motif proteins could give specific biological functions to HP0827. Finally, the study on natural RNA target is also important to completely understand the biological function of HP0827.</P>

A Plant Immune Receptor Detects Pathogen Effectors that Target WRKY Transcription Factors

Sarris, Panagiotis F.,Duxbury, Z.,Huh, S.,Ma, Y.,Segonzac, C.,Sklenar, J.,Derbyshire, P.,Cevik, V.,Rallapalli, G.,Saucet, Simon B.,Wirthmueller, L.,Menke, Frank L.H.,Sohn, K.,Jones, Jonathan D.G. Cell Press ; MIT Press 2015 Cell Vol.161 No.5

Defense against pathogens in multicellular eukaryotes depends on intracellular immune receptors, yet surveillance by these receptors is poorly understood. Several plant nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors carry fusions with other protein domains. The Arabidopsis RRS1-R NB-LRR protein carries a C-terminal WRKY DNA binding domain and forms a receptor complex with RPS4, another NB-LRR protein. This complex detects the bacterial effectors AvrRps4 or PopP2 and then activates defense. Both bacterial proteins interact with the RRS1 WRKY domain, and PopP2 acetylates lysines to block DNA binding. PopP2 and AvrRps4 interact with other WRKY domain-containing proteins, suggesting these effectors interfere with WRKY transcription factor-dependent defense, and RPS4/RRS1 has integrated a ''decoy'' domain that enables detection of effectors that target WRKY proteins. We propose that NB-LRR receptor pairs, one member of which carries an additional protein domain, enable perception of pathogen effectors whose function is to target that domain.