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The establishment of KORCC (KOrean Renal Cell Carcinoma) database
Byun, Seok-Soo,Hong, Sung Kyu,Lee, Sangchul,Kook, Ha Rim,Lee, Eunsik,Kim, Hyeon Hoe,Kwak, Cheol,Ku, Ja Hyeon,Jeong, Chang Wook,Lee, Ji Youl,Hong, Sung Hoo,Kim, Yong-June,Hwang, Eu Chang,Kwon, Tae Gyun The Korean Urological Association 2016 Investigative and Clinical Urology Vol.48 No.6
<P><B>Purpose</B></P><P>The purpose of this article is to report establishment of the 1st Web-based database (DB) system to collect renal cell carcinoma (RCC) data in Korea.</P><P><B>Materials and Methods</B></P><P>The new Web-based DB system was established to collect basic demographic and clinicopahtological characteristics of a large cohort of patients with RCC in Korea. Data from a total of 6,849 patients were collected from 8 tertiary care hospitals that agreed to participate in organizing the Korean Renal Cell Carcinoma (KORCC) study group as of 1 July 2015. Basic demographic and clinicopathological characteristics were collected. The data of patients who underwent surgical treatments were analyzed to characterize Korean RCC.</P><P><B>Results</B></P><P>We established the 1st Web-based DB of Korean RCC, a database comprising renal mass management cases from multiple centers in Korea. The data of 5,281 patients who underwent surgical management (mean follow-up, 32 months) were analyzed. The most common symptom was incidentally detected renal mass (76.9%). Clinical T1a was the most common (54.3%) stage and mean tumor size was 4.8±4.2 cm. Radical nephrectomy accounted for 62.7% of cases and an open approach was used in 50.7% and 52.2% of radical and partial nephrectomies, respectively. The 5-year overall, cancer-specific and recurrence-free survival rates were 88.1%, 92.2%, and 88.0%, respectively.</P><P><B>Conclusions</B></P><P>We report the 1st establishment of a Web-based DB system to collect RCC data in Korea. This DB system will provide a solid basis for the characterization of Korean RCC.</P>
Human extracellular superoxide dismutase (EC-SOD) expression in transgenic chicken
Sung June Byun,Mi Ran Ji,Ye Jin Jang,A In Hwang,Hee Kyoung Chung,Jeom Sun Kim,Kyung Woon Kim,Hak Jae Chung,Byoung Chul Yang,Ik Soo Jeon,Jin Ki Park,Jae Gyu Yoo,Tae Yoon Kim 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.8
Effects of ice-binding protein from Leucosporidium on the cryopreservation of boar sperm
Sang Hyoun Park,Keon Bong Oh,Sun-A Ock,Sung June Byun,Hwi-Cheul Lee,Suresh Kumar,Sung Gu Lee,Jae-Seok Woo 한국수정란이식학회 2018 한국동물생명공학회지 Vol.33 No.3
The aim of this study was performed to evaluate the effects of ice-binding protein from the arctic yeast Leucosporidium (LeIBP) supplementation on cryopreservation of boar sperm. The collected semen was diluted (1.5×108/ml) in lactose egg yolk (LEY) and cooled at 5°C for 3 h. The cooled semen was then diluted (1×108/ml) in LeIBP containing LEY with 9% glycerol and maintained at 5°C for 30 min. The semen was divided into six experimental groups (control, 0.001, 0.005, 0.01, 0.05 and 0.1 mg/ml of LeIBP). The straws were kept on above the liquid nitrogen (LN2) vapors for 20 minutes and then plunged into LN2. After thawing, computer-assisted sperm analysis was used for sperm motility and flow cytometry was performed to assess the viability, acrosome integrity (FITC-PSA/PI), ROS (DCF/PI), lipid peroxidation (BODIPY C11/PI) and apoptosis (Annexin V/PI), respectively. No significant responses were observed for sperm motility. However, sperm viability was significantly increased on 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). In addition, acrosome integrity was significantly increases LeIBP groups (P < 0.05) and both ROS and lipid peroxidation level were lower in all LeIBP groups than those of control (P < 0.05). On the other hand, a significant higher apoptosis rate was observed in 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). It can be assumed that a supplementation of LeIBP in boar sperm freezing extender is an effective method to increase the sperm qualities after cryopreservation.
Byun, Seok-Soo,Hwang, Eu Chang,Kang, Seok Ho,Hong, Sung-Hoo,Chung, Jinsoo,Kwon, Tae Gyun,Kim, Hyeon Hoe,Kwak, Cheol,Kim, Yong-June,Lee, Won Ki Hindawi Publishing Corporation 2016 BioMed research international Vol.2016 No.-
<P><I>Background</I>. The prognostic significance of the neutrophil-to-lymphocyte ratio (NLR) in nonmetastatic renal cell carcinoma (non-mRCC) is controversial, although NLR has been established as a prognostic factor in several cancers. The objective of our study was to assess the prognostic significance of preoperative NLR in non-mRCC, based on a large, multicenter cohort analysis.<I> Methods</I>. Totally, 1,284 non-mRCC patients undergoing surgery were enrolled from six institutions between 2000 and 2014. Recurrence-free survival (RFS) and cancer-specific survival (CSS) were calculated, and the prognostic significance of NLR was evaluated.<I> Results</I>. Patients with higher NLR had larger tumors (<I>p</I> < 0.001), higher T stage (<I>p</I> < 0.001), worse Eastern Cooperative Oncology Group performance status (<I>p</I> < 0.001), worse symptoms (<I>p</I> = 0.003), sarcomatoid differentiation (<I>p</I> = 0.004), and tumor necrosis (<I>p</I> < 0.001). The 5-year RFS and CSS rates were significantly lower in patients with high NLR than in those with low NLR (each <I>p</I> < 0.001). Multivariate analysis identified NLR to be an independent predictor of RFS and CSS (each <I>p</I> < 0.05). Moreover, predictive accuracy of multivariate models for RFS and CSS increased by 2.2% and 4.2%, respectively, with NLR inclusion.<I> Conclusions</I>. Higher NLR was associated with worse clinical behavior of non-mRCC. Also, NLR was a significant prognostic factor of both RFS and CSS.</P>
Effects of Leucosporidium Ice-binding Protein on the Cryopreservation of Boar Sperm
Sang Hyoun Park,Keon Bong Oh,Sun-A Ock,Sung June Byun,Hwi-Cheul Lee,Hyeon Yang,Seongsoo Hwang,Gi-Sun Im,Jae-Seok Woo 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
This study was performed to evaluate effects of Leucosporidium ice-binding protein (LeIBP) supplementation on boar (Duroc) sperm freezing. The collected semen was diluted (1.5×10⁸ /mL) in lactose egg yolk (LEY) and cooled at 5°C for 3 h. The cooled semen was then diluted (1×10⁸ /mL) in LeIBP containing LEY with 9% glycerol and maintained at 5°C for 30 min. The semen was divided into six experimental groups (control, 0.001, 0.005, 0.01, 0.05, and 0.1 mg/mL of LeIBP). The straws were stayed above the liquid nitrogen (LN2) vapors for 20 minutes and then plunged into LN2. After thawing, sperm parameters such as motility, viability, and acrosome integrity, intracellular reactive oxygen species (ROS), apoptosis, and undetected lipid peroxidation were determined. Computer assisted sperm analysis was used for sperm parameters and flowcytometry was performed to acrosome integrity (FITC-PSA/PI), ROS (DCFHDA/ PI), lipid peroxidation (BODIPY C11/PI), and apoptosis (Annexin V/PI), respectively. The differences were not in sperm motility, but in viability of 0.05 and 0.1 mg/mL groups compared to control (p<0.05). Higher acrosome integrity was observed in LeIBP groups compared to control (p<0.05), except for 0.001 group. Both ROS and lipid peroxidation level were lower in all LeIBP groups than that of control (p<0.05). Furthermore, lower apoptosis rate was observed in both 0.005 and 0.1 LeIBP groups compared to control (p<0.05). It can be postulated that a supplementation of LeIBP in boar sperm freezing extender is an effective method to increase the sperm qualities after cryopreservation.
Byun, Kyung Min,Kim, Sung June,Kim, Donghyun The Optical Society 2006 Applied optics Vol.45 No.14
<P>We investigate the effect of the cross-sectional profile of an array of metallic nanowires on the feasibility of a localized surface plasmon resonance (LSPR) biosensor. Calculations were performed using rigorous coupled-wave analysis with an emphasis on the extinction properties of the LSPR structure. The results indicate that the nanowire structure, particularly that of a T-profile, delivers an extremely linear sensing performance over a wide range of the target refractive index with much enhanced sensitivity. The extinction-based LSPR structure also involves a relatively large dimension and thus is expected to provide a feasible biosensor using current semiconductor technology.</P>
Oviduct-Specific Enhanced Green Fluorescent Protein Expression in Transgenic Chickens
BYUN, Sung June,KIM, Sung Woo,KIM, Kyung-Woon,KIM, Jeom Sun,HWANG, In-Sul,CHUNG, Hee Kyoung,KAN, In Sun,JEON, Ik-Soo,CHANG, Won-Kyoung,PARK, Soo-Bong,YOO, Jae Gyu Japan Society for Bioscience, Biotechnology, and A 2011 Bioscience, biotechnology, and biochemistry Vol.75 No.4
<P>In this study, we confirmed the ability of the 2-kb promoter fragment of the chicken ovalbumin gene to drive tissue-specific expression of a foreign EGFP gene in chickens. Recombinant lentiviruses containing the EGFP gene were injected into the subgerminal cavity of 539 freshly laid embryos (stage X). Subsequently the embryos were incubated to hatch using phases II and III of the surrogate shell <I>ex vivo</I> culture system. Twenty-four chicks (G0) were hatched and screened for EGFP with PCR. Two chicks were identified as transgenic birds (G1), and these founders were mated with wild-type chickens to generate transgenic progeny. In the generated transgenic hens (G2), EGFP was expressed specifically in the tubular gland of the oviduct. These results show the potential of the chicken ovalbumin promoter for the production of biologically active proteins in egg white.</P>