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        Global variation in the piggyBac-like element of pink bollworm, Pectinophora gossypiella

        Jianjun Wang,Gregory S. Simmons,Thomas A. Miller,Bruce E. Tabashnik,Yoonseong PARK 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.1

        The piggyBac transposable element, originally discovered in the cabbage looper, Trichoplusia ni, has been used widely in genetic engineering of insects including the pink bollworm, Pectinophora gossypiella, a major lepidopteran pest of cotton. Previously, we identified an intact copy of a piggyBac-like element (PLE) in pink bollworm, designated as PgPLE1.1. Here we report global variation in the occurrence and sequence of PgPLE1.1and its flanking sequences. Low to high frequency of the PgPLE1.1 insertion was observed in populations from USA, Mexico, China, India, and Israel, while there is no PgPLE1.1 insertion in the populations from Australia. Investigation of the five haplotypes of PgPLE1.1, their frequency, and the flanking sequences of PgPLE1.1revealed significant differences of the populations from Australia and China compared to other global populations, although recent occurrences of extensive gene flows among global populations were evident.

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