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- 저자 : Boschen, Suelen Lucio (검색결과 1 건)
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Tracking tonic dopamine levels <i>in vivo</i> using multiple cyclic square wave voltammetry
Oh, Yoonbae,Heien, Michael L.,Park, Cheonho,Kang, Yu Min,Kim, Jaekyung,Boschen, Suelen Lucio,Shin, Hojin,Cho, Hyun U.,Blaha, Charles D.,Bennet, Kevin E.,Lee, Han Kyu,Jung, Sung Jun,Kim, In Young,Lee, Elsevier 2018 Biosensors & bioelectronics Vol.121 No.-
<P><B>Abstract</B></P> <P>For over two decades, fast-scan cyclic voltammetry (FSCV) has served as a reliable analytical method for monitoring dopamine release in near real-time <I>in vivo</I>. However, contemporary FSCV techniques have been limited to measure only rapid (on the order of seconds, <I>i.e.</I> phasic) changes in dopamine release evoked by either electrical stimulation or elicited by presentation of behaviorally salient stimuli, and not slower changes in the tonic extracellular levels of dopamine (<I>i.e.</I> basal concentrations). This is because FSCV is inherently a differential method that requires subtraction of prestimulation tonic levels of dopamine to measure phasic changes relative to a zeroed baseline. Here, we describe the development and application of a novel voltammetric technique, multiple cyclic square wave voltammetry (M-CSWV), for analytical quantification of tonic dopamine concentrations <I>in vivo</I> with relatively high temporal resolution (10 s). M-CSWV enriches the electrochemical information by generating two dimensional voltammograms which enable high sensitivity (limit of detection, 0.17 nM) and selectivity against ascorbic acid, and 3,4-dihydroxyphenylacetic acid (DOPAC), including changes in pH. Using M-CSWV, a tonic dopamine concentration of 120 ± 18 nM (n = 7 rats, ± SEM) was determined in the striatum of urethane anethetized rats. Pharmacological treatments to elevate dopamine by selectively inhibiting dopamine reuptake and to reduce DOPAC by inhibition of monoamine oxidase supported the selective detection of dopamine <I>in vivo</I>. Overall, M-CSWV offers a novel voltammetric technique to quantify levels and monitor changes in tonic dopamine concentrations in the brain to further our understanding of the role of dopamine in normal behavior and neuropsychiatric disorders.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Highly sensitive and selective electrochemical method for detecting tonic dopamine level <I>in vivo</I>. </LI> <LI> Enhanced visualization of the data by two-dimensional voltammogram of electrochemical responses. </LI> <LI> High temporal resolution (10 s). </LI> </UL> </P>
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