http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Tae‐,Jin,Kim, Nayoung,Kim, Eun‐,Ok,Choi, Jong‐,Rip,Bluestone, Jeffrey A.,Lee, Kyung‐,Mi Blackwell Publishing Ltd 2010 Immunology Vol.130 No.4
<P><B>Summary</B></P><P>Natural killer (NK) cells can destroy xenogeneic tissues by antibody‐dependent cell cytotoxicity (ADCC) and direct lysis. Unlike ADCC, activating interactions between human NK receptors and their cognate ligands in pigs are not fully elucidated. We set up this study to identify human NK activating receptors recognizing porcine cells isolated from distinct organs, e.g., aorta, cornea and liver, and to provide a molecular basis for effective immunosuppressive regimens. Among the array of NK receptors tested, NKp46, 2B4, CD49d, CD48, CD2 and NKG2D, only CD2 and NKG2D were shown to be involved in both cytotoxicity and cytokine (interferon‐γ and tumour necrosis factor‐α) production against porcine targets. Simultaneous blocking of CD2 and NKG2D by combining its monoclonal antibodies further suppressed xenogeneic NK responses. Moreover, addition of a suboptimal dose of PD98059, an extracellular signal‐regulated kinase (ERK) kinase inhibitor, to those cells maximally reduced NK cytotoxicity, suggesting that ERK plays an important role in NK‐mediated xenoreactivity. These impairments in NK cells were tightly associated with defective intracellular calcium mobilization and the subsequent degranulation process. Therefore, our data demonstrate a distinct role of CD2 and NKG2D on human NK cells in recognizing porcine grafts and further provide a potentially efficacious combinational regimen using anti‐CD2 and anti‐NKG2D monoclonal antibodies with PD98059</accessionId> in a pig‐to‐human transplantation model.</P>
Kim, Kwanghee,An, Hyo Jin,Jun, Seung-Hyun,Kim, Tae-Jin,Lim, Seon Ah,Park, Gayoung,Na, Hyon Bin,Park, Yong Il,Hyeon, Taeghwan,Yee, Cassian,Bluestone, Jeffrey A,Kim, Jungbae,Lee, Kyung-Mi American Chemical Society 2012 NANO LETTERS Vol.12 No.8
<P>Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP–NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP–NF to specifically isolate, enrich, and activate CD3<SUP>+</SUP> T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8<SUP>+</SUP>CD3<SUP>+</SUP> T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP–NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2012/nalefd.2012.12.issue-8/nl301388d/production/images/medium/nl-2012-01388d_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl301388d'>ACS Electronic Supporting Info</A></P>