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      저자 : Bishajit Sarkar (검색결과 1 건)
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        Virus like particles- A recent advancement in vaccine development

        Bishajit Sarkar,Syed Sajidul Islam,Umme Salma Zohora,Md. Asad Ullah 한국미생물학회 2019 미생물학회지 Vol.55 No.4

        The unintentional reversion of various attenuated and inactivated vaccines has led the way of discovering and development of the virus like particles (VLPs) in the 1970s and 80s. The VLPs contain only the antigenic determinant portion of the structure of a target virus that is able to produce the same immune responses like the original viruses, however, since the particles do not contain any genetic material, the particles can’t infect like the actual viruses. They are able to induce both the humoral and cell mediated immune responses. Since infection doesn’t occur, this technology is a safe and reliable way to combat infectious diseases. Currently, VLPs are produced for a good number of infectious diseases and researches are going on to develop VLPs against many more diseases. Based on the structure, VLPs can be divided into two types: enveloped and non-enveloped VLPs. Enveloped VLPs have greater flexibility. To produce VLPs, various expression systems can be used: yeast, bacterial, mammalian, insect, and plant cells can be used. Insect cell culture system is mainly used in VLP production. The appropriate system should be determined based on the VLP type in question. The production and purification of all the VLPs are quite similar: the cloning of the gene for the antigenic portion, cell harvesting, disruption (if necessary), clarification, concentration and characterization, these steps are done using various means. This review summarizes the types of VLPs, their immunogenic properties, various expression systems that can be used for production and the production procedures of various VLPs.

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