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Bhandari, Prerana,Ahmad, Firoz,Dalvi, Rupa,Koppaka, Neeraja,Kokate, Prajakta,Das, Bibhu Ranjan,Mandava, Swarna Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16
Background: Chromosomal aberrations identified in acute lymphoblastic leukemia (ALL) have an important role in disease diagnosis, prognosis and management. Information on karyotype and associated clinical parameters are essential to physicians for planning cancer control interventions in different geographical regions. Materials and Methods: In this study, we present the overall frequency and distribution patterns of chromosomal aberrations in both children and adult de novo B lineage ALL Indian patients using conventional cytogenetics, interphase FISH and multiplex RT-PCR. Results: Among the 215 subjects, cytogenetic results were achieved in 172 (80%) patients; normal karyotype represented 37.2% and abnormal 62.8% with a distribution as follows: 15.3% hypodiploidy; 10.3% hyperdiploidy; 15.8% t(9;22); 9.8% t(1;19); 3.7% t(12;21); 2.8% t(4;11); 2.8% complex karyotypes. Apart from these, we observed several novel, rare and common chromosomal rearrangements. Also, FISH studies using LSI extra-signal dual-color probes revealed additional structural or numerical changes. Conclusions: These results demonstrate cytogenetic heterogeneity of ALL and confirm that the incidence of chromosomal abnormalities varies considerably. To the best of our knowledge, this is one of the largest reported series of cytogenetic investigations in Indian B-lineage ALL cases. In addition, ongoing cytogenetic studies are warranted in larger groups of B-lineage ALL cases to identify newly acquired chromosomal abnormalities that may contribute to disease diagnosis and management.
Phytonutrient Profile of Purple Perilla (Perilla frutescens var. crispa) Seeds
Bhandari, Shiva Ram,Lee, Ju-Kyong,Lee, Young-Sang The Korean Society of Crop Science 2011 한국작물학회지 Vol.56 No.3
To characterize phytonutrients, the seeds of 12 purple Perilla (Perilla frutescens var. crispa) accessions collected from Korea and Japan were used for quantitative analysis of tocopherols, phytosterols, squalene and fatty acids. The average tocopherol, squalene and phytosterols contents were 12.2 mg $100g^{-1}$, 3.99 mg $100g^{-1}$ and 77.20 mg $100g^{-1}$, respectively. Among 4 tocopherol (T) isomers (${\alpha}$-T, ${\beta}$-T, ${\gamma}$-T, and ${\delta}$-T), ${\gamma}$-T was present in the highest quantity (11.03 mg $100g^{-1}$) with the least variation (CV = 13.7%), while ${\beta}$-T was present in lowest quantity (0.25 mg 1$100g^{-1}$). Compared to campesterol (4.36 mg $100g^{-1}$) and stigmasterol (13.32 mg $100g^{-1}$), ${\beta}$-sitosterol exhibited higher quantity (59.51 mg $100g^{-1}$) with 9.5% of variation. The major fatty acids were unsaturated fatty acids such as linolenic (61.5%), linoleic (17.3%), and oleic (9.9%) acids compared to saturated ones: palmitic (7.6%) and stearic (3.7%) acids. When Korean and Japanese accession were compared, almost no difference in content could be observed, while more variation as evaluated by CV (%) could be observed in Japanese accession in most phytonutrients suggesting wider genetic variation of purple Perilla in Japan. Presence of all above-mentioned phytonutrient compounds strongly suggested health beneficial value of purple Perilla seeds.
Efficient wind fragility analysis of RC high rise building through metamodelling
Bhandari, Apurva,Datta, Gaurav,Bhattacharjya, Soumya Techno-Press 2018 Wind and Structures, An International Journal (WAS Vol.27 No.3
This paper deals with wind fragility and risk analysis of high rise buildings subjected to stochastic wind load. Conventionally, such problems are dealt in full Monte Carlo Simulation framework, which requires extensive computational time. Thus, to make the procedure computationally efficient, application of metamodelling technique in fragility analysis is explored in the present study. Since, accuracy by the conventional Least Squares Method (LSM) based metamodelling is often challenged, an efficient Moving Least Squares Method based adaptive metamodelling technique is proposed for wind fragility analysis. In doing so, artificial time history of wind load is generated by three wind field models: i.e., a simple one based on alongwind component of wind speed; a more detailed one considering coherence and wind directionality effect, and a third one considering nonstationary effect of mean wind. The results show that the proposed approach is more accurate than the conventional LSM based metamodelling approach when compared to full simulation approach as reference. At the same time, the proposed approach drastically reduces computational time in comparison to the full simulation approach. The results by the three wind field models are compared. The importance of non-linear structural analysis in fragility evaluation has been also demonstrated.
Bhandari, Khagendra P.,Roland, Paul J.,Kinner, Tyler,Cao, Yifang,Choi, Hyekyoung,Jeong, Sohee,Ellingson, Randy J. The Royal Society of Chemistry 2015 Journal of Materials Chemistry A Vol.3 No.13
<▼1><P>We use a solution-based bromide anion hot injection method to synthesize stable, phase pure and highly crystalline cubic iron pyrite (FeS2) nanocrystals, with size varying from ∼70 to ∼150 nm.</P></▼1><▼2><P>We use a solution-based hot injection method to synthesize stable, phase pure and highly crystalline cubic iron pyrite (FeS2) nanocrystals, with size varying from ∼70 to 150 nm. We use iron(ii) bromide as an iron precursor, elemental sulfur as the sulfur source, trioctylphosphine oxide (TOPO) and 1,2-hexanediol as capping ligands, and oleylamine (OLA) as a non-coordinating solvent during the synthesis. We report on the influence of hydrazine treatment, and of thermal sintering, on the morphological, electronic, optical, and surface chemical properties of FeS2 films. Four point probe and Hall measurements indicate that these iron pyrite films are highly conductive. Although they are unsuitable as an effective photovoltaic light-absorbing layer, they offer clear potential as a conducting contact layer in photovoltaic and other optoelectronic devices.</P></▼2>
Bhandari, Shiva Ram,Basnet, Sunita,Chung, Kyu-Hwan,Ryu, Ki-Hyun,Lee, Young-Sang 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.2
The aim of this study was to compare the nutritional composition of a genetically modified (GM) CMV-resistant red pepper strain with its parental line. Specifically, the nutrient content (moisture, protein, lipid, ash, carbohydrate, and energy), minerals, fatty acid composition, capsaicinoids (capsaicin and dihydrocapsaicin), free sugars (glucose, sucrose, and fructose), vitamin E isomers (${\alpha}$-, ${\beta}$-, ${\gamma}$-, and ${\delta}$-tocopherols), vitamin C, phytosterols (campesterol, stigmasterol, and ${\beta}$-sitosterol), squalene contents, and ASTA values were analyzed and compared. Most of the analyzed compounds showed no significant differences between the GM red peppers and the parental line. The only significant difference was observed in stigmasterol content, but the difference was below the 15% natural-fluctuation threshold. These results suggest that the CMV-GM pepper is equivalent to its parental line in terms of nutritional and phytochemical composition.
Bhandari, Shiva Ram,Chae, Young,Lee, Jun Gu Korean Society of Horticultural Science 2016 원예과학기술지 Vol.34 No.5
To assess South Korean commercial tomato cultivars, regular and cherry tomato cultivars were grown in the greenhouse and evaluated for color attributes, titratable acidity, pH, total soluble solids, carotenoids (lycopene and ${\beta}$-carotene), total phenols, flavonoids, vitamin C, and antioxidant activity. Significant differences (p < 0.05 using Duncan's multiple range test (DMRT)) were observed in the levels of most phytochemicals, quality parameters, and antioxidant activity among the twenty South Korean tomato cultivars tested. Lycopene and ${\beta}$-carotene contents varied significantly (p < 0.05 using DMRT), from $0.95mg{\cdot}100g^{-1}$ to $5.12mg{\cdot}100g^{-1}$ and $0.65mg{\cdot}100g^{-1}$ to $3.56mg{\cdot}100g^{-1}$ of fresh weight, respectively. ${\beta}$-carotene contents exhibited the highest genetic variation (59.2%), followed by naringenin (52.8%) and other phytochemicals. Most of the cherry tomato cultivars had statistically higher levels (p < 0.05 using DMRT) of carotenoids, phenols, flavonoids, vitamin C, and antioxidant activity compared to the regular tomato varieties, suggesting their higher nutritional value. Lycopene content was highest in the cultivars YoYo, Jicored, Titi-Chal, TY-Endorphin, and Rubyking. Cultivars Rubyking, TY-Endorphin, and Titi-Chal also showed relatively higher antioxidant activities in three assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric ion reducing antioxidant power (FRAP) assays. All the antioxidants, except luteolin, were positively correlated with antioxidant activities; the highest correlation was observed between total phenol and antioxidant activities, followed by the correlation between rutin and vitamin C. Cultivars identified to have superior nutritional status would be useful in tomato breeding programs to further improve quality and health benefits of tomatoes for the fresh and processed markets.
Bhandari, Piyush Kumar,Bissu, Sushil Kumar Korean Mathematical Society 2018 대한수학회논문집 Vol.33 No.4
This paper is devoted to the study of $Tur{\acute{a}}n$-type inequalities for some well-known special functions such as Gauss hypergeometric functions, generalized complete elliptic integrals and confluent hypergeometric functions which are derived by using a new form of the Cauchy-Bunyakovsky-Schwarz inequality. We also apply these inequalities for some sample of interest such as incomplete beta function, incomplete gamma function, elliptic integrals and modified Bessel functions to obtain their corresponding $Tur{\acute{a}}n$-type inequalities.