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      • KCI등재

        High throughput comparative assessment of biofilm formation of Candida glabrata on polystyrene material

        Bindu Sadanandan,Priya Ashrit,Lokesh Kyathsandra Nataraj,Kalidas Shetty,Amruta Puroshottam Jogalekar,Vijayalakshmi Vaniyamparambath,Beena Hemanth 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.5

        Candida glabrata is the second most reported Candida species causing candidiasis next only to Candidaalbicans. Biofilm formation by C. glabrata accelerates pathogenicity, so understanding conditions for biofilm formationin relevant polystyrene polymers is essential. Determining optimum conditions for biofilm formation using one variableat a time (OVAT) is laborious and inaccurate. Therefore, mathematical modelling for design of experiments tooptimize conditions for biofilm formation in Candida species was undertaken. In this study, a simple and robust statisticalmethod, response surface methodology (RSM) was used to optimize independent variables like temperature, incubationperiod, media pH, shaker speed and inoculum density. Biofilm forming conditions were optimized andcompared for two important local clinical isolates and one standard culture on 96 well polystyrene microtiter plates. Biofilm was quantified by different methods, like XTT for cell viability, crystal violet for biofilm, calcofluor white, andwet and dry weight measurements for cell mass. Quantification of cell viability and biofilm indicated heterogeneityamong the three cultures. The results revealed an important finding that foetal bovine serum (FBS) does not significantlyaffect biofilm formation in vitro. This simple high throughput method for optimization and quantification ofCandida biofilm has relevance for applications in rapid screening of anti-Candida biologics and therapeutic solutionswhile advancing control measures in polystyrene carriers in hospital settings.

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