http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Bachan (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Immobilization Imparts Stability to Watermelon Urease to Work in Water Miscible Organic Media
Om Prakash,Lata Sheo Bachan Upadhyay 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.2
The behaviour of alginate immobilized and soluble watermelon (Citrullus vulgaris) urease in water miscible organic solvents like, acetonitrile, dimethylformamide (DMF), ethanol, methanol, and propanol is described. The organic solvents exhibited a concentration dependent inhibitory effect on both the immobilized and the soluble urease in the presence of urea. Pretreatment of soluble enzyme preparations with organic solvents in the absence of substrate for 10 min at 30oC led to rapid loss in the activity, while similar pretreatment of immobilized urease with 50% (v/v) of ethanol, propanol, and acetonitrile was ineffective. Time-dependent inactivation of immobilized urease, both in the presence and in the absence of urea, revealed stability for longer duration of time even at very high concen-tration of organic solvents. The soluble enzyme, on the other hand, was rapidly inactivated even at fairly lower concentrations. The results suggest that the immobilization of watermelon urease in cal-cium alginate make it suitable for its application in organic media. The observations are discussed.
-
Immobilization of Watermelon (Citrullus vulgaris) Urease in Agarose Gel for Urea Estimation
Prakash, Om,Puliga, Srilakshmi,Upadhyay, Lata Sheo Bachan Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.2
Urease from dehusked seeds of watermelon was immobilized in 1.5% agarose gel with 53.9% entrapment. There was negligible leaching(<10% at $4^{\circ}C$) and the same gel membrane could repeatedly be used for seven days. The immobilization exhibited no apparent change in the optimum pH but there was a significant decrease in the optimum temperature ($50^{\circ}C$ as compared to $65^{\circ}C$ for soluble urease). The immobilized urease revealed an apparent $K_m\;of\;9.3{\pm}0.3 M;$ 1.2 times lower than the soluble enzyme $(11.4{\pm}0.2mM)$. Unlike soluble enzyme which was inhibited at 200mM urea, the immobilized urease was inhibited at 600mM of urea and above, and about 47% activity was retained at 2M urea. The time-dependent thermal inactivation kinetics at 48 and $52^{\circ}C$ was found to be biphasic, in which half of the initial activity was destroyed more rapidly than the remaining half. These gel membranes were also used for estimating the urea content of the blood samples from the University hospital. The results obtained matched well with those obtained by the usual method employed in the clinical pathology laboratory. The significance of these observations is discussed.
-
Immobilization Imparts Stability to Watermelon Urease to Work in Water Miscible Organic Media
Prakash, Om,Upadhyay, Lata Sheo Bachan The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.2
The behaviour of alginate immobilized and soluble watermelon (Citrullus vulgaris) urease in water miscible organic solvents like, acetonitrile, dimethylformamide (DMF), ethanol, methanol, and propanol is described. The organic solvents exhibited a concentration dependent inhibitory effect on both the immobilized and the soluble urease in the presence of urea. Pretreatment of soluble enzyme preparations with organic solvents in the absence of substrate for 10 min at $30^{\circ}C$ led to rapid loss in the activity, while similar pretreatment of immobilized urease with 50% (v/v) of ethanol, propanol, and acetonitrile was ineffective. Time-dependent inactivation of immobilized urease, both in the presence and in the absence of urea, revealed stability for longer duration of time even at very high concentration of organic solvents. The soluble enzyme, on the other hand, was rapidly inactivated even at fairly lower concentrations. The results suggest that the immobilization of watermelon urease in calcium alginate make it suitable for its application in organic media. The observations are discussed.
-
Immobilization of Watermelon (Citrullus vulgaris) Urease in Agarose Gel for Urea Estimation
Om Prakash,Srilakshmi Puliga,Lata Sheo Bachan Upadhyay 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.2
Urease from dehusked seeds of watermelon was immobilized in 1.5% agarose gel with 53.9% entrapment. There was negligible leaching (< 10% at 4℃) and the same gel membrane could repeatedly be used for seven days. The immobilization exhibited no apparent change in the optimum pH but there was a significant decrease in the optimum temperature (50℃ as compared to 65℃ for soluble urease). The immobilized urease revealed an apparent Km of 9.3 ± 0.3 mM; 1.2 times lower than the soluble enzyme (11.4 ± 0.2 mM). Unlike soluble enzyme which was inhibited at 200 mM urea, the immobilized urease was inhibited at 600 mM of urea and above, and about 47% activity was retained at 2 M urea. The time-dependent thermal inactivation kinetics at 48 and 52℃ was found to be biphasic, in which half of the initial activity was destroyed more rapidly than the remaining half. These gel membranes were also used for estimating the urea content of the blood samples from the University hospital. The results obtained matched well with those obtained by the usual method employed in the clinical pathology laboratory. The significance of these observations is discussed.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
