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Ä Ö Ü ä ö ü ß
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      저자 : Azam Nasirian (검색결과 2 건)
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      • Rhodamine-based near-infrared probe for emission detection of ATP in lysosomes in living cells

        Tikum, Anjong Florence,Kim, Gyoungmi,Nasirian, Azam,Ko, Jeong Won,Yoon, Juyoung,Kim, Jinheung Elsevier 2019 Sensors and actuators. B, Chemical Vol.292 No.-

        <P><B>Abstract</B></P> <P>A new near infrared fluorescent probe was designed and prepared to detect ATP and target lysosomes in cells. The non-fluorescent probe was turned on during the interaction with ATP, affording a 115-fold increase in fluorescence intensity. The colorless probe turned purple upon interaction with ATP. The probe displayed high sensitivity and selectivity toward ATP compared with other biomolecules, such as AMP, ADP, GMP, and CMP. Such significant spectroscopic changes derive from a structural change of the probe’s spirocyclic moiety to its ring-opened form. This occurs through electrostatic and π,π-stacking interactions between the probe and ATP. This probe was also used in selective lysosome staining and for real-time ATP level monitoring. It exhibits great advantages in cell imaging studies, such as excellent photostability, low cytotoxicity, and good cell membrane permeability.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new near infrared fluorescent probe was reported to detect ATP and target lysosomes in cells. </LI> <LI> Probe <B>1</B> was used in selective lysosome staining and for real-time ATP level monitoring. </LI> <LI> Probe <B>1</B> exhibits excellent photostability, low cytotoxicity, and good cell membrane permeability. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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        Mixed ligand octahedral Zn(II) complex of N^N^O donor tridentate Schiff base ligand and N^N donor bidentate bipyridine ligand: Synthesis, characterization, biological activity and cytotoxicity

        Thamilarasan Vijayan,Arumugam Jayamani,Mani Pugazhenthi,Azam Nasirian,Jinheung Kim,Gopinath Kasi 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.11

        Tridentate NNO donor Schiff base ligand and its mixed ligand zinc(II) complexwere synthesized stoichiometrically and characterized by electronic, infrared,mass spectral techniques and elemental analysis. To understand the DNA bindingability, the complex was investigated by various analytical and spectroscopictechniques in presence of calf thymus DNA (CT-DNA). The bindingstudies showed that the zinc complex interacts with DNA by groove bindingmode with intrinsic binding strength of 2.11 105 M1 and with bovine serumalbumin the complex showed dynamic quenching attributed for changes inthe secondary structure of BSA with efficient interaction. The MCF-7 cell linestudies of the zinc(II) complex with 90.8 μM IC50 value revealed that the complexis effective for the breast cancer cell line and has a potential as anticancerdrug. Furthermore, the tridentate ligand and its mixed ligand zinc complexwere screened for antibacterial and antifungal activities, which showed betterantimicrobial activity for complex.

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