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Identification of Pinus L.Based on Pollen Morphology and Pollen Isozymes
Kim, Kae Hwan,F.A.Aravanopoulos,L.Zsuffa 전북대학교 농업과학기술연구소 1995 農大論文集 Vol.26 No.1
소나무屬의 잣나무亞屬과 소나무亞屬에속하는 6 種 24 營養系에 對하여 光學顯微鏡에 依하여 花粉形態를 觀察하였고, 電氣泳動에 依하여 同位酵素를 調査하였다. 多變量分析에 의한 花粉의 形態學的 parameters는 部分的으로 分類學的 價値를 보여주었다. 또한 잣나무亞屬과 소나무亞屬의 花粉으로부터 抽出物 分析 結果, 및 개의 同位酵素는 소나무屬의 系統的 同定에 利用될 수 있었다. EST, GOT, IDH, MDH, PGI, 6PGD 등의 酵素들은 反復되는 banding pattern을 보여주었고 調査된 20개 遺傳子座 中 13개遺傳子座0는 2 亞屬과 6 種을 判別하는데 도움이 되었다. The pollen morphology and pollen isozymes of 24 clones from 6 Pinus species belonging to subgenera Haploxylon and Diploxylon were investigated by light microscopy and electro-phoresis. Pollen morphological parameters subjected to multivariate statistical analyses showed their limited taxonomic value. Isozyme patterns from extracts of the two pinus subgenera indicated that several isozymes can be useful in systematic identification of pinus. Six enzyme systems showed repeatable banding patterns. These were: EST, GOT, IDH, MDH, PGI and 6PGD. Thirteen of the 20 loci scored could be used to discriminate between the two subgenera and the 6 species studied.
김계환,Zsuffa, L.,Aravanopoulos, F A 한국임학회 1997 한국산림과학회지 Vol.86 No.2
The pollen morphology of 20 species and one variety of the two genera of Hippocastanaceae was investigated by LM, SEM and TEM. The pollen grains of this family are monads, prolate to subprolate, isopolar, radially symmetrical, 3-colporate or 3-colpate and ora is circular or lolongate. Sculpture pattern is distinctly striate or spinulose, Brae in various directions. Based on the aperture types and sculpture patterns three major pollen types of this family are discernible : hippocastanum, indica, and punduana.