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        Partial Purification and Characterization of Polyphenol Oxidase from Chinese Parsley (Coriandrum sativum)

        Hanbin Lin,Allan Wee Ren Ng,Chen Wai Wong 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.suppl1

        Purification and characterization of polyphenol oxidase (PPO) from Chinese parsley (Coriandrum sativum) were achieved. Crude PPO exhibited an enzyme activity of 1,952.24 EU/mL. PPO was partially purified up to 6.52x with a 10.89% yield using gel filtration chromatography. Maximal PPO activity was found at 35oC, pH 8.0 for 4-methylcatechol and at 40oC, pH 7.0 for catechol. PPO showed a higher affinity towards 4-methylcatechol, but a higher thermal stability when reacting with catechol. LCysteine was a better inhibitor than citric acid for reducing PPO activity at concentrations of 1 and 3mM in the presence of either substrate. Two 46 kDa isoenzymes were identified using SDS-PAGE. Isolation and characterization of Chinese parsley serves as a guideline for prediction of enzyme behavior leading to effective prevention of enzymatic browning during processing and storage, including inhibition and inactivation of PPO.

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