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        Mites (Acari) associated with Rhynchophorus ferrugineus (Coleoptera: Curculionidae) in Malaysia, with a revised list of the mites found on this weevil

        Masilamany Dilipkumar,Ali Ahadiyat,PeterMašán,Tse Seng Chuah 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.2

        There is a great deal of diversity among phoretic association particularly in mesostigmatic mites that exploited insect host to complete their dispersal strategy. Similarly, the red palm weevil, Rhynchophorus ferrugineus, also has been used as a carrier by the phoretic mites. In this study, we found Centrouropoda almerodai (Uropodidae), Macrocheles mammifer, Macrocheles cf. oigru (Macrochelidae), Uroobovella assamomarginata and Uroobovella javae (Dinychidae) as the phoreticmites associated with the Malaysian red palm weevils. Male weevils had significantly greater number of mites per host as compared to the female weevils. Present study revealed that the red palm weevils were infested with very large numbers of phoretic mites which occurmainly under the elytra. Our results combined with those in the literatures suggest the potential role of phoresy in the evolution of parasitism.

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        Phototactic behavior of Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae) under visible wavelengths

        Fakhari Hajar,Karimzadeh Javad,Moharramipour Saeid,Ahadiyat Ali,Doranian Davoud 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

        The greenhouse whitefly, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae), is one of the most important pests of greenhouse crops. The intensive use of chemical insecticides has resulted in insecticide resistance in T. vaporariorum and the critical level of pesticides residue in crops. It is therefore necessary to develop new control methods based on ecological pest management. The present study was designed to control greenhouse whitefly by finding and using insect repellent wavelengths. The repellent wavelength experiment was conducted by a two-way phototactic apparatus given a choice between darkness and visible wavelength spectrum from violet (380–450 nm) to red (620–750 nm). The phototactic responses of the greenhouse whitefly were then investigated in a four-way phototactic apparatus given a choice between two light regimes, light-emitting diode (LED) and sunlight. The results indicated that the lowest (69.2%) and highest (97.8%) number of whiteflies were attracted to violet and orange (590–625 nm) spectra, respectively. In addition, the present study indicated a significant attraction of T. vaporariorum adults to sunlight compared with LED. Furthermore, the eggplants grown under growth LEDs showed a significantly higher growth rate than the plants grown under sunlight. These findings suggest that this type of LED not only has positive effects on plant growth but it also has a repellent activity on T. vaporariorum adults, leading us to develop an effective behavioral control of the greenhouse whitefly.

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        Wisteria Vein Mosaic Virus Detected for the First Time in Iran from an Unknown Host by Analysis of Aphid Vectors

        Hajar Valouzi,Seyedeh-Shahrzad Hashemi,Stephen J,Wylie,Ali Ahadiyat,Alireza Golnaraghi 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.1

        The development of reverse transcription–polymerase chain reaction using degenerate primers against conserved regions of most potyviral genomes enabled sampling of the potyvirome. However, these assays usually involve sampling potential host plants, but identifying infected plants when they are asymptomatic is challenging, and many plants, especially wild ones, contain inhibitors to DNA amplification. We used an alternative approach which utilized aphid vectors and indicator plants to identify potyviruses capable of infecting common bean (Phaseolus vulgaris). Aphids were collected from a range of asymptomatic leguminous weeds and trees in Iran, and transferred to bean seedlings under controlled conditions. Bean plants were tested serologically for potyvirus infections four-weeks post-inoculation. The serological assay and symptomatology together indicated the presence of one potyvirus, and symptomology alone implied the presence of an unidentified virus. The partial genome of the potyvirus, encompassing the complete coat protein gene, was amplified using generic potyvirus primers. Sequence analysis of the amplicon confirmed the presence of an isolate of Wisteria vein mosaic virus (WVMV), a virus species not previously identified from Western Asia. Phylogenetic analyses of available WVMV sequences categorized them into five groups: East Asian-1 to 3, North American and World. The Iranian isolate clustered with those in the World group. Multiple sequence alignment indicated the presence of some genogroup-specific amino acid substitutions among the isolates studied. Chinese isolates were sister groups of other isolates and showed higher nucleotide distances as compared with the others, suggesting a possible Eastern-Asian origin of WVMV, the main region where Wisteria might have originated.

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