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- 저자 : Alexandre Mboene Noah (검색결과 2 건)
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Micropropagation of cocoyam (Xanthosoma sagittifolium L. Schott) in temporary immersion bioreactor
Nicolas Niemenak,Alexandre Mboene Noah,Denis Ndoumou Omokolo 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
This study aims at establishing a temporaryimmersion technique for large-scale propagation ofcocoyam (Xanthosoma sagittifolium). Sucrose was experimentedwith as a determinant factor for shoot multiplicationin this culture system. The highest proliferation rate(68 ± 7) occurred with 20 g l-1 sucrose in the culturemedium. This concentration appeared to be the optimalamount due to its promoting effect on plantlet development. The acclimatized plantlets showed a continuouseffect of sucrose treatment during ex vitro growth, especiallyin low sucrose concentration. This fact is evidencedby the low survival rate (0.13 ± 0.12) and the poor chlorophyllcontent (1.180 ± 0.076 mg g-1) recorded onplantlets derived from 15 g l-1 of sucrose. The treatmentwith 60 g l-1 of sucrose prior to acclimatization was efficientfor roots induction and elongation. The analysis of pHrevealed a fluctuation from one subculture to another, withan overall pH decrease under all treatments tested and,thus, indicates that plants release proton during growth. This feature had an impact on in vitro plantlets growth. Thepotentiality of the temporary immersion technique to fosterthe production of Xanthosoma sagittifolium is discussed.
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Micropropagation of cocoyam (Xanthosoma sagittifolium L. Schott) in temporary immersion bioreactor
Niemenak, Nicolas,Noah, Alexandre Mboene,Omokolo, Denis Ndoumou 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
This study aims at establishing a temporary immersion technique for large-scale propagation of cocoyam (Xanthosoma sagittifolium). Sucrose was experimented with as a determinant factor for shoot multiplication in this culture system. The highest proliferation rate ($68{\pm}7$) occurred with $20gl^{-1}$ sucrose in the culture medium. This concentration appeared to be the optimal amount due to its promoting effect on plantlet development. The acclimatized plantlets showed a continuous effect of sucrose treatment during ex vitro growth, especially in low sucrose concentration. This fact is evidenced by the low survival rate ($0.13{\pm}0.12$) and the poor chlorophyll content ($1.180{\pm}0.076mgg^{-1}$) recorded on plantlets derived from $15gl^{-1}$ of sucrose. The treatment with $60gl^{-1}$ of sucrose prior to acclimatization was efficient for roots induction and elongation. The analysis of pH revealed a fluctuation from one subculture to another, with an overall pH decrease under all treatments tested and, thus, indicates that plants release proton during growth. This feature had an impact on in vitro plantlets growth. The potentiality of the temporary immersion technique to foster the production of Xanthosoma sagittifolium is discussed.
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