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      저자 : Alessandra Norici (검색결과 2 건)
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        Impact of inhibitors of amino acid, protein, and RNA synthesis on C allocation in the diatom Chaetoceros muellerii: a FTIR approach

        Giordano, Mario,Norici, Alessandra,Beardall, John The Korean Society of Phycology 2017 ALGAE Vol.32 No.2

        Fourier Transform Infrared (FTIR) spectroscopy was used to study carbon allocation patterns in response to N-starvation in the nearly ubiquitous diatom Chaetoceros muellerii. The role of gene expression, protein synthesis and transamination on the organic composition of cells was tested by using specific inhibitors. The results show that inhibition of key processes in algal metabolism influence the macromolecular composition of cells and and prior cell nutritional state can influence a cell's response to changing nutrient availability. The allocation of C can thus lead to different organic composition depending on the nutritional context, with obvious repercussions for the trophic web. This also shows that C allocation in algal cells is highly flexible and that C (and the energy associated with its allocation) can be variably and rapidly partitioned in algal cells in response to relatively short term perturbations. Furthermore, the data confirm and extend the utility of infrared spectroscopy as a probe of the metabolic state of autotrophic cells.

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        Impact of inhibitors of amino acid, protein, and RNA synthesis on C allocation in the diatom Chaetoceros muellerii: a FTIR approach

        Mario Giordano,Alessandra Norici,John Beardall 한국조류학회I 2017 ALGAE Vol.32 No.2

        Fourier Transform Infrared (FTIR) spectroscopy was used to study carbon allocation patterns in response to N-starvationin the nearly ubiquitous diatom Chaetoceros muellerii. The role of gene expression, protein synthesis and transaminationon the organic composition of cells was tested by using specific inhibitors. The results show that inhibitionof key processes in algal metabolism influence the macromolecular composition of cells and and prior cell nutritionalstate can influence a cell’s response to changing nutrient availability. The allocation of C can thus lead to different organiccomposition depending on the nutritional context, with obvious repercussions for the trophic web. This also shows thatC allocation in algal cells is highly flexible and that C (and the energy associated with its allocation) can be variably andrapidly partitioned in algal cells in response to relatively short term perturbations. Furthermore, the data confirm andextend the utility of infrared spectroscopy as a probe of the metabolic state of autotrophic cells.

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