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        Bacterial Cellulose Production by Komagataeibacter xylinus Using Rice-washed Water and Tofu Processing Wastewater with the Addition of Sodium Glutamate

        Yoice Srikandace,Arina Yuthi Apriyana,Siti Aisyah Zahrad,Wahyu Ramdhani,Putri Putih Puspa Asri,Dian Andriani,Akbar Hanif Dawam Abdullah,Anung Syampurwadi,Rahmat Satoto,Myrtha Karina 한국섬유공학회 2022 Fibers and polymers Vol.23 No.5

        Various industrial and agricultural waste have been using for bacterial cellulose (BC) production. This study aimsto apply the rice-washed water and tofu processing wastewater as a growth medium to synthesize BC replacing Hestrin-Schramm (HS) medium by Komagataeibacter xylinus with the addition of sodium glutamate. The fermentation was carriedout under static conditions for 5, 10, and 15 days at room temperature. The characteristics of BC were analyzed by SEM,XRD, and UTM for mechanical properties, whereas the yield was calculated from the dry weight. SEM images confirmed theformation of dense rod-shaped nanofibers configuration, and X-ray diffraction analysis revealed that BC had a typicalcrystalline form of the cellulose Iα. The yield from rice-washed water (RW) and tofu processing wastewater (TW) wasslightly higher than from the HS medium. The highest yield of BC from RW, TW, and HS medium on the day 15 was 2.63 g/l,3.80 g/l, and 2.55 g/l, respectively. Therefore, rice-washed water and tofu processing wastewater can use as a replacement forthe role of Hestrin-Schraam (HS) medium. The yield increased when the medium was enriched with 1 % (w/v) sodiumglutamate, became 4.47 g/l, 4.63 g/l, and 3.37 g/l for RW, TW, and HS, correspondingly. However, the addition of sodiumglutamate lowered the tensile strength, Young’s modulus, and crystallinity. Both liquid wastewaters are considered as lowcostand sustainable alternative resources for BC production.

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