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RISS 인기검색어
- 검색키워드
- 저자 : Afsaneh Golkar-Narenji (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
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Effects of intraperitoneal administration of Papaver rhoeas L. extract on mouse ovaries
Afsaneh Golkar-Narenji,Hussein Eimani,Firooz Samadi,Saeid Hasani,Abdol hossein Shahverdi,Poopak Eftekhari-Yazi,Mohammad Kamalinejad 한국통합생물학회 2013 Animal cells and systems Vol.17 No.2
This research studies the effect of water-alcohol Papaver rhoeas L. (P. rhoeas) extract on mouse ovaries and in vitro development (IVD) of oocytes. Different dosages of P. rhoeas extract (25, 50, 100, 200 mg/kg body weight) were injected intraperitoneally (i.p.) during a period of 12 days. Following superovulation, the numbers of ovulated oocytes, the rates of in vitro fertilization, IVD and the cellularity of blastocysts were recorded. Additionally, effect of the best dosage on ovarian follicle population and the ability of immature oocytes to mature in vitro were evaluated. Administration of 200 mg/kg significantly increased the percentage of 48 cells, morula and blastocyst embryos compared to the control group (pB0.05). Furthermore, total cellularity of blastocysts was significantly higher with the administration of 200 mg/kg of extract in comparison to control group (pB0.05). Therefore, the most effective dosage was considered to be 200 mg/kg. With the administration of 200 mg/kg no marked changes were observed in the IVM rate of retrieved oocytes from treated group in comparison to control group. Furthermore, the percentage of ovarian follicles was not significantly different when compared to control group. Also, during visual evaluations no abnormal apoptosis was detected in follicles of ovaries treated with 200 mg/kg when compared to control group. Higher IVD and blastocyst cellularity in the group treated with defined dosage of P. rhoeas indicates that the extract affects ovaries in a dose dependent manner. The extract possibly increases the quality of ovulated oocytes and IVD competence of oocytes.
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Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries
Maryam Zand-vakili,Hussein Eimani,Afsaneh Golkar-Narenji,Poopak Eftekhari-Yazdi,Abdolhosein Shahverdi,Paul Edward Mozdziak 한국통합생물학회 2016 Animal cells and systems Vol.20 No.5
One of the most important factors affecting survival rate of ovarian follicles during transplantation period is proper vascular development. The objective of the study was to evaluate the effect of vascular endothelial growth factor (VEGF) on auto-transplanted ovarian tissue. Twenty-one-dayold female mice (n = 30) transplanted as control group and 21-day-old female mice (n = 40) were divided into 4 groups that were treated with 0.5, 1, 2 and 4 μg/mL of VEGF directly injected to auto-transplanted ovarian tissue. Twenty-one days after transplantation, mice were treated with 7.5 IU pregnant mare serum gonadotropin and human chorionic gonadotropin. Transplanted ovaries were removed and sections were prepared from transplanted tissues for staining. The most effective dosage of VEGF on transplanted tissue was determined over H&E (hematoxylin and eosin) staining results. Slides were compared using TUNEL staining and CD31 assay for the most effective dosage. The percentages of preantral and antral follicles were not significantly different between transplanted group with 4 μg/mL VEGF and non-transplanted group. Lower apoptotic areas and higher CD31 expression were observed in transplanted ovaries treated with 4 μg/mL VEGF when compared to transplanted ovaries without VEGF treatment. VEGF positively affects the quality of ovarian tissue during transplantation. Survival rate of follicles and follicular development has improved with the effect of VEGF.
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